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School of Biosciences

Invitrogen Qubit 4.0 Fluorometer

The Qubit 4.0 accurately measure the concentration of target molecules using dye-specific reagents that will bind to DNA, RNA or protein.

New assays can be created for other analytes using the MyQubit Assay Design Tool. A new feature for the latest Qubit model is determining the RIN score for RNA.

This approach means there is a low background from other molecules that may be in your sample for example salts from PCR master mixes or DNA present in RNA extractions making the assays specific and sensitive.

Each assay is set up using a similar workflow in a couple of minutes and the readings take a few seconds for each sample.

Applications include:

  • RNA input measurement for Next generation sequencing (NGS)or qPCR
  • DNA input measurements for NGS and assessing the success of workflows
  • Assessing MiRNA in samples
  • Protein detection
  • Specific analyte detection of choice

Key Features

  • Depending on your assay and sample concentration you can use as little as 1ul per sample up to 20ul/sample for each measurement.
  • Stores up to 1000 sample results.
  • USB thumb drive and cable for data transfer to Excel™ software and software downloads.

The assays are based on the emission and detection of Fluouresence at specific wavelengths when the dye binds to its target molecules. Each assay is stable for up to 3 hours and performed at room temperature.

The available assays are listed below:

Invitrogen Qubit dsDNA HS Assay Kits

  • Input concentrations 10 pg/uL to 100 ng/uL.

Invitrogen Qubit dsDNA BR Assay Kits

  • Input concentrations 100 pg/uL to 1,000 ng/uL.

Invitrogen Qubit ssDNA Assay Kit

  • Input concentrations 50 pg/uL to 200 ng/uL.

Invitrogen Qubit RNA High Sensitivity (HS) Assay Kits

  • Input concentrations 0.2 to 200 ng/uL.

Invitrogen Qubit RNA Broad Range (BR) Assay Kits

  • Input concentrations 0.5 to 1200 ng/uL.

Invitrogen Qubit RNA XR Assay Kits

  • Input concentrations 5 to 20,000 ng/uL.

Invitrogen Qubit microRNA Assay Kit

  • Input concentration 0.025–150 ng/µL.

Invitrogen Qubit Protein Assay Kits

  • The Qubit Protein Assay Kit is highly selective for proteins and is also highly accurate in the presence of reducing reagents, but not in the presence of large amounts of detergent. Common contaminants, such as reducing reagents (e.g., DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, and DNA (but not detergents), are well tolerated in the assay. Slight protocol modifications are required for other contaminants.
  • Will detect concentrations between 12.5 µg⁄mL to 5 mg⁄mL with low protein-to-protein variation.

Invitrogen Qubit Protein BR Assay

  • Input concentration 100 µg⁄mL to 20 mg⁄mL.

MyQubit Assays

  • Various preconfigured assays or custom assays.

Users can book the equipment by emailing biosi-GRH@cardiff.ac.uk.

Contact Genomics Research Hub for details of costs at BIOSI-GRH@cardiff.ac.uk.

We offer full in house training for all applications, all users need to have been trained by Genomics Research Hub staff before using the Qubit without supervision.

To comply with Cardiff University Health and Safety Policy, individuals must receive appropriate training before using any of the equipment within the Genomics Research Hub and be aware of the risks involved in its use.

Furthermore, for use of any of the sequencers, users must:

  • Receive training from a member of Genomics Research Hub staff (indirect training from other users will not be sufficient).
  • Read and understand the latest Risk Assessment for the Genomics Research Hub.

If top-up training is required, please notify Hub staff when making a booking and this can be provided prior to your planned session.

All users of the Genomics Research Hub are expected to observe the following. If they fail to comply they will lose their access rights:

  • Food and drink are not permitted within the facility.
  • All waste material (including laboratory gloves) should be properly disposed of in the clinical waste bins provided.
  • Coats and bags should not be brought into the laboratories. Leave them in the adjoining office.
  • Do not use any equipment that you have not been properly trained for.  All users should familiarise themselves with the appropriate risk assessments for the procedures being undertaken.
  • All USB data storage devices must be virus-checked before plugging them into any of our systems.