Bioimaging Research Hub

The Bioimaging Research Hub provides state-of-the-art digital imaging equipment and technical expertise for the examination of biological samples.

The Hub provides the following technical services: digital photography, macroscopic imaging, conventional microscopical techniques (brightfield, darkfield, phase contrast, differential interference contrast, polarising and epifluorescence), whole slide scanning, and super-resolution confocal laser scanning microscopy.

Equipment

Name	Make/Model	Details
Confocal laser scanning microscope with super-resolution capability	Zeiss LSM 880 upright confocal microscope with Airyscan	State-of-the-art, fast scanning super-resolution confocal system with fluorescence correlation spectroscopy (FCS) and fluorescence lifetime imaging (FLIM)
Confocal laser scanning microscope with upright and inverted stands	Leica TCS SP2 AOBS confocal system	The SP2 system comprises upright (Leica DM6000) microscope and inverted (Leica DMIRE2) microscopes that share a Leica laser scan-head, fast scanning galvonometre stage and Jenoptik ProgRes digital camera. The inverted microscope has a Solent Scientific incubation system for live cell imaging.
Spinning Disc Confocal Microscope	Olympus IX71 motorised, inverted microscope	Crest Optics X-Light V2 confocal imager; Cairn Research tri-line laser bank (405nm; 488nm; 561nm); Hamamatsu ORCA Flash 4 CMOS digital camera with M-View Gemini image splitter
Zeiss Lightsheet Z.1	Zeiss Lightsheet Z.1	Fully-integrated turn-key system for fluorescence lightsheet microscopy with environmental control.
X-CLARITY Tissue Clearing System	Logos Biosystems X-CLARITY	The X-CLARITY tissue clearing system is a collection of automated systems and ready-to-use reagents designed to clear biological tissues for downstream imaging applications.
Multiwavelength bioluminescence / fluorescence imaging system	Biospace Lab PhotonIMAGER Optima	Real-time, optical imaging system (bioluminescence and fluorescence) for kinetic studies and longitudinal experiments of synthetic systems/ models and living tissues.
Imaris PC workstation	Bitplane Imaris 9.3 C1 for Cell Biologists	PC workstation running Imaris 9.3 C1 for Cell Biologists. This is a software package of advanced rendering and analysis tools for 3D/4D microscopy datasets suitable for researchers requiring a broad range of functionality to study cells and tissues.
Zeiss PALM MicroBeam Laser Micro-dissector	Zeiss PALM MicroBeam laser micro-dissector	Fully motorised Zeiss Axio Observer microscope with brightfield and epifluorescence. RoboMover automated collection device. Frequency tripled solid state 355nm laser Zeiss AxioCam colour digital camera
Upright, fully-automated, multimodal research microscope	Olympus BX61 microscope with Hamamatsu ORCA Spark digital camera	Suitable for brightfield, differential interference contrast and epifluorescence imaging. The system is fully motorised and has a stepping stage that allows acquisition of 3D image data series.
Upright multimodal research microscope	Olympus BX40 microscope with Hamamatsu ORCA Spark digital camera	Suitable for brightfield, darkfield, phase contrast and epifluorescence imaging.
Upright research microscope	Olympus BX50	Suitable for brightfield and epifluorescence imaging.
Upright brightfield microscope	Leica DMRB brightfield photomicroscope	Suitable for general brightfield photomicroscopy.
Slide scanning microscope	Objective Imaging Surveyor Slide Scanning System	The microscope allows whole slide scanning and mosaic stitching of histological sections under brightfield illumination.
Automated high-throughput slide scanning microscope	Olympus VS200 Slide Scanning System	The microscope allows automated high-throughput whole slide scanning and stitching of histological sections under brightfield, phase contrast, darkfield, polarisation and multiplexed epifluorescence (DAPI, FITC, Cy3 & Cy5) illumination.
Stereo-zoom imaging system	Olympus SZX12 stereo zoom system	Suitable for brightfield and epifluorescence imaging of small samples such as whole-mounts, culture plates, gels or large tissue sections.
Macro-zoom imaging system	Meiji macro-imaging system	Suitable for brightfield imaging of small samples such as whole-mounts, culture plates, gels or large tissue sections.
Ultimaker 3 Extended 3D printer	Ultimaker 3 Extended 3D printer	Layer plastic deposition (LPD)-type 3D printer; Work field of 215 x 215 x 300mm; Printing resolution of 20-200 microns Z-suite software accepts .stl, .obj, .dxf and .3mf file types.
Zortrax M200 3D printer	Zortrax M200 3D printer	Layer plastic deposition (LPD)-type 3D printer; Work field of 200 x 200 x 180mm; Printing resolution of 90-400 microns Z-suite software accepts .stl, .obj, .dxf and .3mf file types
Thermo Scientific NanoDrop 3300 Fluorospectrometer	Thermo Scientific NanoDrop 3300 Fluorospectrometer	Microvolume fluorospectrometer; Three light emitting diodes (LEDs): Ex Max: UV: 365; Blue: 470nm; White: 460-650nm Spectral resolution: 8nm; Measurement time: 2-10 seconds
Digital photography	Nikon D90 digital SLR	The Nikon D90 is suitable for general-purpose photography or macroimaging.