Flow Cytometry
------------------------------ PAGE BREAK ------------------------------
The flow cytometry unit is situated in room W/4.02, there are two machines within the facility: a BD FACS Canto and BD FACS Aria.
FACS Canto
Individuals must be trained on this machine prior to use. Training takes approximately one hour. Once trained users can use the machine unassisted.
FACS Aria
The BD FACS Aria is a bench top cell sorter. This machine can only be used with the assistance of the facility supervisor.
Bookings
Availability can be checked with the online booking form (see link below). Users will be charged from the start of the booking slot. The FACS machines are available for booking between 09.00 and 17.00 and the facility can only be accessed with a swipe card and key, these can be obtained from the facility supervisor in room C/4.20.
A minimum of 24 hours notice must be given to avoid penalty charges.
Charges
Canto and Aria charges are illustrated in the following table, these tariffs may be subject to change.
| Canto | Internal | External (academic) | External (industry) |
|---|---|---|---|
| Training | £15 | £25 | £60 |
| Without operator | £20 | £35 | £50 |
| With operator | £30 | £45 | £90 |
| Cancellation fee (less than 24 hour notice) | £15 | £25 | £40 |
| Off-line analysis | £15 | £25 | £50 |
| Aria | Internal | External (academic) | External (industry) |
|---|---|---|---|
| Training | N.A. | N.A. | N.A. |
| Setup charge | £25 | £35 | £50 |
| Without operator | £25 | N.A. | N.A. |
| With operator | £50 | £75 | £100 |
| Cancellation fee (less than 24 hour notice) | £25 | £35 | £50 |
| Off-line analysis | £25 | £35 | £50 |
Further Information
Consumables
It is important that cells are filtered before using the FACS machines in order to avoid cell clumps sticking to the interior of the instruments. Below is a list of filters that are currently used.
| Supplier | Description | Pack size | Price |
|---|---|---|---|
| BD | Sterile 40 m m cell strainers (50ml tubes) | 50 | £30 |
| Celltrics | Sterile 30 m m cells strainers (15ml tubes) | 50 | £60 |
| Celltrics | Non-sterile 30 m m cells strainers (15ml tubes) | 250 | £60 |