Research Microscopes and Cryostat
A number of microscopes and a cryostat are available to BIOSI researchers on a charge per session basis (see below for details).
Please note. Users must undergo a training session and demonstrate basic competence before they are granted access to the instruments. For further details regarding training and instrument access please contact Jim Ralphs.
Leica DMRB. Suitable for general brightfield photomicroscopy. The instrument has x1.6, x2.5, x5, x10, x20 and x40 dry lenses and a x100 oil immersion lens and is equipped with a colour Moticam 2000 digital camera and basic image acquisition software. Located in Biosi 2, E4.24 (Connective Tissue Biology Labs - CTBL)
Olympus BX40. Suitable for brightfield, phase contrast and epifluorescence imaging. The instrument has x4, x10, x20 and x40 dry lenses and a x100 oil immersion lens and RGB filters for epifluorescence. The microscope is equipped with a SIS F-View 2 black and white digital camera and Olympus Cell^P imaging software. Located in Biosi 2, E4.24 (CTBL)
Olympus BX61. Suitable for brightfield, D.I.C and epiluorescence imaging. The instrument has x4, x10, x20, x40 and x100 (oil) lenses and RGB filters for epifluorescence. It is equipped with a SIS F-view B&W digital camera and AnalySIS imaging software. Located in Biosi 2, E4.24 (CTBL). N.B. Bookings can only be made via Jim Ralphs.
Olympus BX41M. Suitable for epifluorescence imaging.The instrument has x5, x10, x20 and x40 dry lenses and a x100 oil immersion lens and RGB filter cubes. The microscope is equipped with an F-View black and white digital camera with Olympus Cell^P imaging software. Located in Biosi 2, E0.03 (Confocal Unit)
Cryostat. Used for cutting sections of frozen tissue for producing microscope slides. Particularly used where wax-based processing is not compatible with a particular immunohistochemical technique or where results are needed rapidly. Located in Biosi 2 E4.17 Open Plan area (CTBL). There are two cryostats - only the right-hand-side one is available for general use.
Booking and Charges
The cryostat, the Leica DMRB, the Olympus BX40 and the Olympus BX41M microscopes can all be booked via their respective online calendars which are linked on the right-hand side of this page. Bookings for the Olympus BX61can only be made via Jim Ralphs (after training you will be issued a username and password to access the BX61 Online booking page).
All of the instruments are booked on a 'per session' basis with three sessions available per day: 8am-11am, 11am-2pm and 2pm-5pm. Each session has a fixed charge of £5.
Cancellations
If you cannot make your booking you should delete your entry on the on-line booking forms as soon as possible to make the session available for another user. If you fail to show up without cancelling your booking you will be charged for the session.
Page content written and maintained by Dr. A.J. Hayes and Mr. M. Isaacs