Fluorochromes
Spectral Properties of Common Flurochromes
| Fluorochrome | Ex (max) | Em (max) |
| Acridine Orange | 500 (+DNA) 460 (+RNA) | 526 (+DNA) 650 (+RNA) |
| Alexa 405 | 402 | 421 |
| Alexa 430 | 434 | 539 |
| Alexa 488 | 495 | 519 |
| Alexa 500 | 503 | 525 |
| Alexa 514 | 518 | 540 |
| Alexa 532 | 531 | 554 |
| Alexa 546 | 556 | 573 |
| Alexa 555 | 555 | 565 |
| Alexa 568 | 578 | 603 |
| Alexa 594 | 590 | 617 |
| Alexa 610 | 612 | 628 |
| Alexa 633 | 632 | 647 |
| Alexa 635 | 633 | 647 |
| BCECF | 482 (low pH) 503 (high pH) | 520 (low pH) 528 (high pH) |
| BOBO, BOPRO | 462 | 481 |
| BODIPY FL | 505 | 513 |
| BODIPY TMR-X | 542 | 574 |
| BODIPY TR-X | 589 | 617 |
| Calcein | 494 | 517 |
| 6-Carboxyrhodamine 6G | 525 | 555 |
| Cascade Blue | 400 | 420 |
| Cascade Yellow | 402 | 545 |
| DAPI | 358 | 461 |
| DiI | 549 | 565 |
| Eosin | 524 | 544 |
| ER-Tracker | 372 | 556 |
| Ethidium Bromide | 518 | 605 |
| Fluo-3 | 506 | 526 |
| Fluo-4 | 494 | 516 |
| Fluoroscein (FITC) | 494 | 518 |
| Fura-2 | 363 (low Ca2+) 335 (high Ca2+) | 512 (low Ca2+) 505 (high Ca2+) |
| Fura Red | 472 (low Ca2+) 436 (high Ca2+) | 657 (low Ca2+) 637 (high Ca2+) |
| Hoechst 33258, 33342 | 352 | 461 |
| Lissamine Rhodamine B | 570 | 590 |
| Lucifer Yellow CH | 428 | 536 |
| LysoTracker Green | 504 | 511 |
| MitoTracker Green | 490 | 516 |
| Nile Red | 549 | 628 |
| Oregon Green 488 | 496 | 524 |
| Oregon Green 514 | 511 | 530 |
| Pacific Blue | 410 | 455 |
| POPO-1, PO-PRO-1 | 434 | 456 |
| Propidium Iodide | 536 | 617 |
| Rhodamine Red | 570 | 590 |
| R-phycoerythrin | 565 | 575 |
| Rhodamine 123 | 507 | 529 |
| SYTOX Blue | 445 | 470 |
| SYTO Green | 504 | 523 |
| SYTO Orange | 547 | 570 |
| TRITC | 555 | 580 |
| Texas Red | 595 | 615 |
| TOTO-1, TO-PRO-1 | 514 | 533 |
| TOTO-3, TO-PRO-3 | 642 | 660 |
| YOYO-1, YO-PRO-1 | 491 | 509 |
| YOYO-3, YO-PRO-3 | 612 | 631 |
Page content written and maintained by Dr. A.J. Hayes and Mr. M. Isaacs