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Fluorochromes

 Spectral Properties of Common Flurochromes

Fluorochrome Ex (max) Em (max)
Acridine Orange500 (+DNA)

460 (+RNA)
526 (+DNA)

650 (+RNA)
Alexa 405402421
Alexa 430434539
Alexa 488495519
Alexa 500503525
Alexa 514518540
Alexa 532531554
Alexa 546556573
Alexa 555555565
Alexa 568578603
Alexa 594590617
Alexa 610612628
Alexa 633632647
Alexa 635633647
BCECF482 (low pH)

503 (high pH)
520 (low pH)

528 (high pH)
BOBO, BOPRO462481
BODIPY FL505513
BODIPY TMR-X542574
BODIPY TR-X589617
Calcein494517
6-Carboxyrhodamine 6G525555
Cascade Blue400420
Cascade Yellow402545
DAPI358461
DiI549565
Eosin524544
ER-Tracker372556
Ethidium Bromide518605
Fluo-3506526
Fluo-4494516
Fluoroscein (FITC)494518
Fura-2363 (low Ca2+)

335 (high Ca2+)
512 (low Ca2+)

505 (high Ca2+)
Fura Red472 (low Ca2+)

436 (high Ca2+)
657 (low Ca2+)

637 (high Ca2+)
Hoechst 33258, 33342352461
Lissamine Rhodamine B570590
Lucifer Yellow CH428536
LysoTracker Green504511
MitoTracker Green490516
Nile Red549628
Oregon Green 488496524
Oregon Green 514511530
Pacific Blue410455
POPO-1, PO-PRO-1434456
Propidium Iodide536617
Rhodamine Red570590
R-phycoerythrin565575
Rhodamine 123507529
SYTOX Blue445470
SYTO Green504523
SYTO Orange547570
TRITC555580
Texas Red595615
TOTO-1, TO-PRO-1514533
TOTO-3, TO-PRO-3642660
YOYO-1, YO-PRO-1491509
YOYO-3, YO-PRO-3612631

 

Page content written and maintained by Dr. A.J. Hayes and Mr. M. Isaacs