Skip to content
Skip to navigation menu

 

Prof Paola Borri  -  PhD


Coherent Anti-Stokes Raman Scattering microscopy for non-invasive imaging of living cells

Optical microscopy is an indispensable tool for cell biology. Different microscopy methods are currently available and continuous effort is devoted to develop new techniques with improved sensitivity, selectivity and/or spatial resolution. An important issue in recent microscopy is the ability to perform non-invasive studies avoiding the need for fluorescent probes that are prone to photobleaching and can perturb the cell functions.

Together with Prof. Wolfgang Langbein who is appointed in the School of Physics and Astronomy, we are developing a new generation of laser-scanning multiphoton microscope based on Coherent Anti-Stokes Raman Scattering (CARS, see figure below) with improved sensitivity and chemical specificity for real-time studies on living cells. Such an instrument allows non-invasive microscopic examination of cells and subcellular structures under physiological conditions without the need to stain or express tagged proteins.

My research plan is to develop a new generation of laser-scanning multiphoton microscope based on Coherent Anti-Stokes Raman Scattering (CARS).

My research plan is to develop a new generation of laser-scanning multiphoton microscope based on Coherent Anti-Stokes Raman Scattering (CARS).

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Recent outputs of this work have been published in:

Andreas Zumbusch, Wolfgang Langbein and Paola Borri, “Nonlinear vibrational microscopy applied to lipid biology” Progress in Lipid Research 52, 615-632 (2013)

Francesco Masia, Adam Glen, Phil Stephens, Paola Borri, and Wolfgang Langbein, "Quantitative Chemical Imaging and Unsupervised Analysis Using Hyperspectral Coherent Anti-Stokes Raman Scattering Microscopy" Anal. Chem. 85, 10820-10828 (2013). Open access

Pope I, Langbein W, Watson P, Borri P "Simultaneous hyperspectral differential-CARS, TPF and SHG microscopy with a single 5 fs Ti:Sa laser" Optics Express 21, 7096 (2013). Open access

Bei Li, Paola Borri, Wolfgang Langbein “Dual/differential coherent anti-Stokes Raman scattering module for multiphoton microscopes with a femtosecond Ti:sapphire oscillator” J. Biomed. Opt. 18, 066004 (2013) Open access

Israel Rocha-Mendoza, Paola Borri,Wolfgang Langbein “Quadruplex CARS micro-spectroscopy” Journal of Raman Spectroscopy 44, 255 (2013)

Claudia Di Napoli, Francesco Masia, Iestyn Pope, Cees Otto, Wolfgang Langbein, and Paola Borri “Chemically-specific dual/differential CARS micro-spectroscopy of saturated and unsaturated lipid droplets” J. Biophotonics  7, 68-76 (2014, online 2012). Open access

Wolfgang Langbein , Israel Rocha-Mendoza, Paola Borri “Single source coherent anti-Stokes Raman micro-spectroscopy using spectral focusing” Applied Physics Letters 95, 081109 (2009).

Israel Rocha-Mendoza, Wolfgang Langbein, Peter Watson, and Paola Borri "Differential coherent anti-Stokes Raman scattering microscopy with linearly-chirped femtosecond laser pulses", Optics Letters, 34 2258 (2009).

Wolfgang Langbein, Israel Rocha-Mendoza, and Paola Borri, “Coherent anti-Stokes Raman micro-spectroscopy using spectral focusing: Theory and Experiment”, Journal of Raman Spectroscopy 40, 800 (2009).

Israel Rocha-Mendoza, Wolfgang Langbein, and Paola Borri, “Coherent antistokes Raman microspectroscopy using spectral focusing with glass dispersion”Applied Physics Letters 93, 201103 (2008).

 

Other research interests

  • Nonlinear optical microscopy (Four-Wave Mixing Imaging) using semiconductor or metallic colloidal nanoparticles 
  • Sensitive molecular and microparticle detection by whispering-gallery modes of coated microspheres
  • Ultrafast coherent dynamics of semiconductor quantum dot materials and devices
  • Time-resolved Förster Resonance Energy Transfer (TR FRET) as probe of biomolecular interactions
Ultrafast coherent laser spectroscopy (four-wave mixing) of InGaAs/GaAs quantum dots with heterodyne detection. Dephasing times measured at different temperatures indicated in red 
[see P. Borri et al. Phys. Rev. Lett. 87, 157401 (2001)]

Ultrafast coherent laser spectroscopy (four-wave mixing) of InGaAs/GaAs quantum dots with heterodyne detection. Dephasing times measured at different temperatures indicated in red

[see P. Borri et al. Phys. Rev. Lett. 87, 157401 (2001)]

Grants

Ministry of Defence – Dstl
BBSRC, EPSRC, MRC
DTI

Group members

Iestyn Pope, Research Associate
(School of Biosciences, Cardiff)

George Zorinyants, Research Associate
(School of Biosciences, Cardiff)

Francesco Masia , Research Associate
(School of Physics and Astronomy, Cardiff)

Claudia Di Napoli, PhD student
(School of Biosciences, Cardiff)

Craig McPhee, PhD student
(School of Biosciences, Cardiff) 

Lukas Payne, PhD Student
(School of Biosciences, Cardiff)

 Josephine Bradley PhD Student
(School of Biosciences, Cardiff)

Kez Cleal, PhD Student
(School of Biosciences, Cardiff)

Ali Naeem, PhD Student
(School of Physics and Astronomy, Cardiff)

Arnica Karuna PhD Student
(School of Physics and Astronomy, Cardiff)

Robin Islam PhD Student
(School of Physics and Astronomy, Cardiff)

 

Collaborators

Wolfgang Langbein
(Photons and Matter - School of Physics and Astronomy, Cardiff)

Peter Watson
(Molecular Biosciences – School of Bioscience, Cardiff)

Trevor Dale 
(Molecular Biosciences – School of Bioscience, Cardiff)

Adrian Harwood 
(Molecular Biosciences  – School of Bioscience, Cardiff)

Arwyn Jones

(School of Pharmacy)

Phil Stephens

(School of Dentistry)

Karl Swann

(School of Medicine)