Protein Technology Research

Equipment and services

Protein expression

The Protein Research Hub is home to a number of facilities which support protein expression applications:

  • Expression vectors and host organisms
  • Prokaryotic protein expression facilities
  • Eukaryotic protein expression facilities (mammalian, yeast and insect cell culture)

Protein purification

Researchers can access the following protein purification facilities:

  • FPLC facility (Akta Pure 25, Akta Explorer, Akta Prime Plus, Akta Start FPLC systems). Full training is provided.
  • Preparative gel electrophoresis (Bio-Rad prep cell)

Protein analysis

The Hub is equipped with state-of-the-art protein analysis instruments:

  • BMG Clariostar monochromator microplate reader
    • Measures absorbance, fluorescence and luminescence in 96- or 384-well plates
    • Continuously adjustable bandwidths (8 to 100 nm) and wavelengths (320 to 850 nm) for absorbance and fluorescence scanning and custom excitation/emission selection
    • Equipped with 2 sample injectors allowing rapid measurements (eg calcium assays)
  • LiCor Odyssey CLx infra-red fluorescence scanner
    • Quantitative Western blotting and gel analysis
    • In-cell Western blot measures protein levels from fixed cells in multi-well plates (ideal for cell line generation or siRNA screening)
  • Malvern MicroV zetasizer light scattering and refractive index detector
    • Measures protein size, concentration and molecular mass following gel filtration chromatography; measures protein size in batch mode

Other facilities available in the hub include cell disruption, a cuvette fluorimeter, and protein analysis using transmission electron microscopy.

Contact us

For more information on our Protein Technology Hub, please contact:

Dr Mark Young

Dr Mark Young

Senior Lecturer

Email:
youngmt@cardiff.ac.uk
Telephone:
+44 (0)29 2087 9394
Dr Colin Berry

Dr Colin Berry

Reader

Email:
berry@cardiff.ac.uk
Telephone:
+44 (0)29 2087 4508