Skip to content
Professor Paola Borri

Professor Paola Borri

Professor, Coordinator of the European Marie Curie ETN consortium MUSIQ

School of Biosciences

School of Physics and Astronomy

Email:
borrip@cardiff.ac.uk
Telephone:
+44 (0)29 2087 9356
Fax:
+44 (0)29 2087 4116
Location:
Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX
Available for postgraduate supervision

Research activities in my group cover the area of biophotonics at the interface between life and physical sciences. In collaboration with the School of Physics and Astronomy, my research work includes:

  • development of next generation laser-scanning multiphoton microscopes based on Coherent Raman Scattering (CRS) for label-free studies on living cells and tissues
  • development of novel optical microscopy techniques for imaging and tracking single nanoparticles background free inside cells
  • development of optical biosensors for sensitive detection of biomolecules using plasmonics sensing with metallic nanoparticles or photonic cavities
  • time-resolved fluorescence resonance energy transfer (TR-FRET) as a probe of biomolecular interactions

News

See our research highlights recently published:

Interested in joining my lab as a self-funded post-graduate student or a postdoc/fellow?  Please contact me by email.

I did my undergraduate in Physics at the University of Florence (Italy) and then received the Laurea (MSc equivalent) summa cum laude and the Ph.D degree in Physics in 1993 and 1997 respectively. From 1997 to 1999 I was Assistant Research Professor at the Technical University of Denmark (Kgs.Lyngby, Denmark).

From 1999 to 2004 I worked as Senior Scientist and EU Marie Curie Fellow (2001-2003) at the Physics Department of Dortmund University in Germany where I received the Habilitation degree in Physics (Venia Legendi) in 2003. During this time I was interested in the experimental investigation of the optical properties of novel semiconductor nanostructures, such as quantum wells and quantum dots. In particular, I developed a new technique for the ultra-fast coherent laser spectroscopy of these nanostructures.

From September 2004 I moved to Cardiff University as Senior Lecturer. On August 1st 2007 I was promoted to Reader and on August 1st 2011 to a Personal Chair.

Honours and awards

In 2015 I received the Royal Society Wolfson Research Merit Award. Jointly funded by the Wolfson Foundation and the Department for Business, Innovation and Skills (BIS), the scheme aims to provide universities with additional support to enable them to attract science talent from overseas and retain respected UK scientists of outstanding achievement and potential.

I received the Marie Curie Excellence Award from the European Commission during the official award ceremony at the Ecole Polytechnique Federale de Lausanne on the 16th November 2006.

Marie Curie Excellence Awards (EXA) aim to give public recognition to outstanding past achievements of scientists who have reached a level of exceptional excellence in their given field. Up to five prizes of 50 000 Euros each are awarded every year.

Professional memberships

In 2010-2015 I was an EPSRC Leadership Fellow.

Since 2014 I am Fellow of the Learned Society of Wales.

2019

2018

2017

2016

2015

2014

2013

2012

2011

2010

2009

2008

2007

2006

2005

2004

2003

2002

2001

2000

1999

1998

  • Borri, P., Langbein, W. W. and Hvam, J. M. 1998. Ultrafast spectroscopy of semiconductor devices. Presented at: 10th International Symposium on Ultrafast Phenomena in Semiconductors, Vilnius, Lithuania, August-September 1998 Presented at Asmontas, S. and Dargys, A. eds.Ultrafast phenomena in semiconductors : proceedings of the 10th International Symposium on Ultrafast Phenomena in Semiconductors (10-UFPS), held in Vilnius, Lithuania, August/September 1998. Materials Science Forum Vol. 297-8. Zurich: Trans TechPublications pp. 67-71.
  • Langbein, W. W., Borri, P. and Hvam, J. M. 1998. Coherent exciton and biexciton nonlinearities in semiconductor nanostructures: effects of disorder. Presented at: 10th International Conference on Ultrafast Phenomena in Semiconductors, Vilnius, Lithuania, August-September 1998 Presented at Steponas, A. and Dargys, A. eds.Ultrafast Phenomena in Semiconductors: Proceedings of the 10th International Symposium on Ultrafast Phenomena in Semiconductors (10-UFPS), held in Vilnius, Lithuania, August/September 1998. Materials Science Forum Vol. 297-8. Zurich: Trans Tech Publications pp. 73-78.
  • Gurioli, M.et al. 1998. Exciton formation and relaxation in GaAs epilayers. Physical Review B 58(20), pp. R13403-R13406. (10.1103/PhysRevB.58.R13403)

1997

1996

1995

Projects

Coherent Raman Scattering microscopy for label-free imaging of living cells and tissues

Optical microscopy is an indispensable tool for cell biology. Different microscopy methods are currently available and continuous effort is devoted to develop new techniques with improved sensitivity, selectivity and spatial resolution. An important issue in recent microscopy is the ability to perform non-invasive studies avoiding the need for fluorescent probes that are prone to photobleaching and can perturb the cell structure and function.

Together with Prof. Wolfgang Langbein in the School of Physics and Astronomy, we have developed a range of home-built laser-scanning multiphoton microscopes based on Coherent Raman Scattering (CRS), enabling rapid label-free chemically specific microscopy of living cells and tissues. A second-generation CRS microscope located in the School of Biosciences has been specifically built to perform multimodal correlative CRS, two -photon fluorescence (TPF) and second harmonic generation (SHG) microscopy for applications in cell biology. This system enables hyperspectral CRS acquisition which has led to the development of a quantitative image analysis tool to distinguish the composition of chemical components and retrieve their concentration and spatial distribution.

With these tools, we have determined the lipid uptake of fixed and living adipose derived human stem cells differentiating into pre-adipocytes, the lipid content and spatial distribution in live mammalian oocytes and early embryos, demonstrated a high-throughput high-content platform for drug screens, and quantitatively measured masses of lipids, proteins and DNA during cell division. We have also shown that CRS can be used to visualise single non-fluorescing nanodiamonds in cells for the first time (see Publications).

Resonant Four-Wave Mixing Imaging with gold nanoparticles

Imaging and tracking single nanoparticles using optical microscopy are powerful techniques with many applications in biology, chemistry, and material sciences. Applied methods to achieve contrast are dominantly fluorescence based, with fundamental limits in the emitted photon fluxes arising from the excited-state lifetime as well as photobleaching. 

We have developed a new technique, based on four-wave mixing (FWM) interferometry, whereby single non-fluorescing gold nanoparticles (AuNPs) are imaged background-free even inside highly heterogeneous cellular environments, owing to their specific nonlinear plasmonic response, and their position is determined with nanometric precision in 3D. The technique is also uniquely sensitive to particle asymmetries of only 0.5% ellipticity, corresponding to a single atomic layer of gold, as well as particle orientation and chirality.

With this technique, we are investigating a number of AuNP-ligand-fluorophore conjugates and their integrity inside cells, using AuNPs as small as 5nm in radius and correlative FWM/confocal fluorescence imaging. The technique opens the prospect to an unprecedented level of understanding of the intracellular fate of single small AuNPs and their trafficking within complex 3D architectures inside living cells (see Publications).

Other research interests

  • Quantitative optical extinction microscopy of individual nano-objects
  • Ultrafast coherent dynamics of semiconductor quantum dot materials and devices
  • Optical biosensing by exploiting plasmonic resonances or photonic cavities
  • Time-resolved Förster Resonance Energy Transfer (TR FRET) as probe of biomolecular interactions

Grants

  • BBSRC, EPSRC, MRC, EU, Ministry of Defence – Dstl, DTI

Group members

  • Iestyn Pope, Research Associate (School of Biosciences, Cardiff)
  • Francesco Masia, Research Fellow (School of Biosciences, Cardiff)
  • Yisu Wang, Research Associate (School of Biosciences, Cardiff)
  • Tual MonfortResearch Associate (School of Biosciences, Cardiff)
  • Barbara Santos GomesResearch Associate (School of Biosciences, Cardiff)
  • Ryan Lewis PhD student (School of Biosciences, Cardiff)
  • Joseph Williams PhD student (School of Biosciences, Cardiff)
  • Samuel Hamilton, PhD student (School of Biosciences, Cardiff)
  • Dafydd HarlowPhD Student (School of Biosciences, Cardiff)
  • Dale BoormanPhD student (School of Biosciences, Cardiff)
  • David ReganPhD Student (School of Physics and Astronomy, Cardiff)
  • Alexander Nahmad-RohenPhD Student (School of Physics and Astronomy, Cardiff)
  • Zoltan SztranyovszkyPhD Student (School of Physics and Astronomy, Cardiff)
  • Rhod Thomas, PhD student (School of Biosciences, Cardiff)
  • Nadhia Monim, PhD student (School of Biosciences, Cardiff)
  • Nicole Slesiona, MUSIQ ESR Fellow and PhD Student (School of Biosciences, Cardiff)
  • Vikramdeep Singh, MUSIQ ESR Fellow and PhD Student (School of Physics and Astronomy, Cardiff)

Collaborators