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Biophotonics Laboratory

This laboratory provides Coherent anti-stokes Raman scattering (CARS) microscopy of living cells, two-photon fluorescence microscopy, four-wave mixing imaging, Raman spectroscopy and time-resolved fluorescence spectroscopy.

Equipment

Name Make/Model Details
CW Laser source at 1064nm Laser 2000 MLIII 1064 DPSS module 1064nm wavelength, power 1.5W
CW Laser source at 420nm Coherent OBIS 421 nm wavelength, power 100mW
CW Laser source at 532m Laser Quantum GEM 532nm wavelength, power 2W
CW Laser source at 660nm Laser Quantum Ignis 660nm wavelength, power 750mW
Multimodal microscope for bright-field, dark-field, DIC, epifluorescence, confocal Raman, TPF, SHG and CARS Nikon Ti-U inverted microscope; Cambridge Technologies Model 6210HSM40 Galvanometer Scanner; Hamamatsu PMT 2x H7422-40, 2x H10721-210 & 2x H10770A-40; Horiba Jobin Yvon iHR550 spectrometer; Andor Technologies DU 971N EMCCD Camera; Homebuilt electronics, optomechanics and software -Inverted microscope stand, 0.72 NA dry condenser, 1.4NA oil condeser, 10X 0.3NA objective, 20X 0.75NA objective, 40x 0.95 NA objective, 60x 1.27NA water objective, 100x 1.45 oil objective, Prior Lumen fluorescence lamp, Hamamatsu ORCA camera, xy-motorised Prior stage, motorised z. Environmental chamber for live cell imaging at 37degree and 5% CO2 -Galvo XY Scanner, 6mm Mirror Set Protected Silver Coating, Flatness of λ/4, XY Mount and Driver -H7422-40 cooled PMT with 40% QE at 500nm, H10721-210 uncooled PMT with 40% QE at 400nm, H10770A-40 uncooled PMT with 40% QE at 500nm -Imaging spectrometer, Czerny-Turner type, f/6.4, length 550mm, automized slit, 2 turrets 3 gratings each. Turret #1: 150, 600 and 1800 lines/mm. Turret #2: 100, 200, 300 lines/mm -95% QE, Electron Multiplying CCD (EMCCD), 1600 x 400 array of 16μm² pixels with thermoelectric cooling down to -100°C
Multimodal microscope for bright-field, dark-field, DIC, TPF, SHG, CARS, SRS, heterodyne CARS and FWM Nikon Ti-U inverted microscope; Cambridge Technologies Model 6210HSM40 Galvanometer Scanner; Mad City Laboratory Nano LP200 sample stage; 2x Zurich Instrument HF2LI Lock-In Amplifier; Hamamatsu PMT H10770A-40 , H10721-210 & H10721-20; Homebuilt electronics, optomechanics and software -Inverted microscope stand, 0.72 NA dry condenser, 1.4NA oil condeser, 10X 0.3NA objective, 20X 0.75NA objective, 40x 0.95 NA objective, 60x 1.27NA water objective, 100x 1.45 oil objective -Galvo XY Scanner, 6mm Mirror Set Protected Silver Coating, Flatness of λ/4, XY Mount and Driver -XYZ movement over 200x200x200micron, 0.4nm resolution, approx. 5ms response time -0.7uHz-50MHz fully digital (210MHz sampling) 1MHz update rate -H10770A-40 uncooled PMT with 40% QE at 500nm, H10721-210 uncooled PMT with 40% QE at 400nm, H10721-20 uncooled PMT with 15% QE at 500nm
Pulsed Laser Source of sub-10fs duration Venteon Pulse-One sub 10fs Pumped by CW 6W 532nm, Delivering sub10fs pulses at 80MHz rep rate centred at 850nm with 300nm bandwidth, more than 500mW average power
Pulsed tuneable laser source Newport/Spectra Physics Mai-Tai and OPO Pulsed laser source providing 100fs pulses at 80MHz with variable wavelengths and four simultaneous outputs: 820nm with >200mW (Mai Tai fundamental), 410nm with >20mW(Mai Tai SHG undepleted), 500nm to 700nm tunable with >300mW @550nm (OPO signal), 950nm to 1500nm with >100mW @1100nm (OPO idler)
Time-correlated single photon counting electronics (TCSPC) Becker & Hickl GmbH SPC-150 TCSPC module One SPC-150 photon counting module, two DCC-100 control units, 3 Amplifiers, one Router. It features ps resolution, Reversed start/stop: Laser repetition rates up to 150 MHz, Saturated count rate 10 MHz, Total useful recorded count rate up to 5 MHz, Dead time 100 ns

Get in touch

Professor Paola Borri

Email:
borrip@cardiff.ac.uk
Telephone:
+44 (0)29 2087 9356

Location

  • Sir Martin Evans Building
    Museum Avenue
    CF10 3AX