Multimodal microscope for bright-field, dark-field, DIC, epifluorescence, confocal Raman, TPF, SHG and CARS

Make/model	Nikon Ti-U inverted microscope; Cambridge Technologies Model 6210HSM40 Galvanometer Scanner; Hamamatsu PMT 2x H7422-40, 2x H10721-210 & 2x H10770A-40; Horiba Jobin Yvon iHR550 spectrometer; Andor Technologies DU 971N EMCCD Camera; Homebuilt electronics, optomechanics and software
Details	-Inverted microscope stand, 0.72 NA dry condenser, 1.4NA oil condeser, 10X 0.3NA objective, 20X 0.75NA objective, 40x 0.95 NA objective, 60x 1.27NA water objective, 100x 1.45 oil objective, Prior Lumen fluorescence lamp, Hamamatsu ORCA camera, xy-motorised Prior stage, motorised z. Environmental chamber for live cell imaging at 37degree and 5% CO2 -Galvo XY Scanner, 6mm Mirror Set Protected Silver Coating, Flatness of λ/4, XY Mount and Driver -H7422-40 cooled PMT with 40% QE at 500nm, H10721-210 uncooled PMT with 40% QE at 400nm, H10770A-40 uncooled PMT with 40% QE at 500nm -Imaging spectrometer, Czerny-Turner type, f/6.4, length 550mm, automized slit, 2 turrets 3 gratings each. Turret #1: 150, 600 and 1800 lines/mm. Turret #2: 100, 200, 300 lines/mm -95% QE, Electron Multiplying CCD (EMCCD), 1600 x 400 array of 16μm² pixels with thermoelectric cooling down to -100°C
Facility	Biophotonics Laboratory
School	School of Biosciences

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