
Yr Athro Phil Stephens
Deon Rhyngwladol ac Ymgysylltu y Coleg, Athro Bioleg Celloedd
- Email:
- stephensp@cardiff.ac.uk
- Telephone:
- +44 (0)29 2074 2529
- Sylwebydd y cyfryngau
2008 - present Head of Tissue Engineering & Reparative Dentistry, School of Dentistry, Cardiff University, Cardiff. 2008 - present Professor of Cell Biology, Wound Biology Group, Tissue Engineering & Reparative Dentistry, School of Dentistry, Cardiff University, Cardiff. 2004 - 2008 Reader in Cell Biology, Wound Biology Group, Dept. Oral Surgery, Medicine & Pathology, School of Dentistry, Cardiff University, Cardiff. 2002 - 2004 Senior Lecturer in Cell Biology, Dept. Oral Surgery, Medicine & Pathology, Dental School, UWCM, Cardiff. 1998 - 2002 Lecturer in Cell Biology, Dept. Oral Surgery, Medicine & Pathology, Dental School, UWCM, Cardiff. Apr - Dec 1997 Secondment to the Molecular Hematopoiesis Laboratories of Professor Corey Largman, UCSF, San Francisco, USA to investigate the role of novel homeobox genes in scarless wound healing. 1994 - 1998 Post-Doctoral Research Fellow, Dept. Oral Surgery, Medicine & Pathology, Dental School, UWCM, Cardiff. 1990 - 1994 SERC (CASE) PhD studentship in the Dept. Biochemistry and Molecular Biology, University of Leeds, Leeds. Memberships: External Activities: Personal Prizes: Supervised Student/PostDoc Prizes:Career Profile
Teaching Profile
Memberships / External Activities
Awards and Prizes
Aelodaethau proffesiynol
British Society of Oral and Dental Research, European Tissue Repair Society
Safleoedd academaidd blaenorol
2008 – 2010 Head of Tissue Engineering & Reparative Dentistry, School of Dentistry, Cardiff University, Cardiff;
2004 – 2008 Reader in Cell Biology, Wound Biology Group, Dept. Oral Surgery, Medicine & Pathology, School of Dentistry, Cardiff University, Cardiff;
2002 – 2004 Senior Lecturer in Cell Biology, Dept. Oral Surgery, Medicine & Pathology, Dental School, UWCM, Cardiff;
1998 – 2002 Lecturer in Cell Biology, Dept. Oral Surgery, Medicine & Pathology, Dental School, UWCM, Cardiff
2018
- Masia, F.et al. 2018. Label-free quantitative chemical imaging and classification analysis of adipogenesis using mouse embryonic stem cells. Journal of Biophotonics 11(7), article number: e201700219. (10.1002/jbio.201700219)
- Caley, M.et al. 2018. Development and characterisation of a human chronic skin wound cell line - towards an alternative for animal experimentation. International Journal of Molecular Sciences 19(4), article number: 1001. (10.3390/ijms19041001)
- Hidalgo San Jose, L.et al. 2018. Microfluidic encapsulation supports stem cell viability, proliferation and neuronal differentiation. Tissue Engineering Part C Methods 24(3), pp. 158-170. (10.1089/ten.TEC.2017.0368)
2016
- Locke, M., Davies, L. C. and Stephens, P. 2016. Oral mucosal progenitor cell clones resist In Vitro myogenic differentiation. Archives of Oral Biology 70, pp. 100-110. (10.1016/j.archoralbio.2016.06.013)
- Howard-Jones, R. A.et al. 2016. Integration-free reprogramming of lamina propria progenitor cells. Journal of Dental Research 95(8), pp. 882-888. (10.1177/0022034516637579)
- Perkins, B. L.et al. 2016. The life science exchange: A case study of a sectoral and sub-sectoral knowledge exchange programme. Health Research Policy and Systems 14, article number: 32. (10.1186/s12961-016-0105-4)
- Morgan, A. J.et al. 2016. Simple and versatile 3D printed microfluidics using fused filament fabrication. PLoS ONE 11(4), article number: e0152023. (10.1371/journal.pone.0152023)
2015
- Roper, J. A.et al. 2015. Ultrasonic stimulation of mouse skin reverses the healing delays in diabetes and aging by activation of Rac1. Journal of Investigative Dermatology 135(11), pp. 2842-2851. (10.1038/jid.2015.224)
- Board-Davies, E.et al. 2015. Oral mucosal lamina propria-progenitor cells exert antibacterial properties via the secretion of osteoprotegerin and haptoglobin. Stem Cells Translational Medicine 4(11), pp. 1283-1293. (10.5966/sctm.2015-0043)
2014
- Stephens, P. and Davies, L. C. 2014. Oral mucosal progenitor cells. In: Vishwakarma, A. et al. eds. Stem Cell Biology and Tissue Engineering in Dental Sciences.. Academic Press, pp. 297-306., (10.1016/B978-0-12-397157-9.00025-4)
- Peake, M. A.et al. 2014. Identification of a transcriptional signature for the wound healing continuum. Wound Repair and Regeneration 22(3), pp. 399-405. (10.1111/wrr.12170)
- McInnes, R. L.et al. 2014. Contrasting host immuno-inflammatory responses to bacterial challenge within venous and diabetic ulcers. Wound Repair and Regeneration 22(1), pp. 58-69. (10.1111/wrr.12133)
2013
- Stephens, P., Caley, M. and Peake, M. 2013. Alternatives for animal wound model systems. Methods in Molecular Biology 1037, pp. 177-201. (10.1007/978-1-62703-505-7_10)
- Masia, F.et al. 2013. Quantitative chemical imaging and unsupervised analysis using hyperspectral coherent anti-Stokes Raman scattering microscopy. Analytical Chemistry 85(22), pp. 10820-10828. (10.1021/ac402303g)
2012
- Wildeboer, D.et al. 2012. Specific protease activity indicates the degree of Pseudomonas aeruginosa infection in chronic infected wounds. European Journal of Clinical Microbiology & Infectious Diseases 31(9), pp. 2183-2189. (10.1007/s10096-012-1553-6)
- Wagstaffe, S. J.et al. 2012. Bispecific antibody-mediated detection of the staphylococcus aureus thermonuclease. Analytical Chemistry 84(14), pp. 5876-5884. (10.1021/ac203403d)
- Davies, L. C.et al. 2012. Oral mucosal progenitor cells are potently immunosuppressive in a dose-independent manner. Stem Cells and Development 21(9), pp. 1478-1487. (10.1089/scd.2011.0434)
2011
- Stephens, P. 2011. Dysfunctional wound healing in chronic wounds. In: Farrar, D. ed. Advanced Wound Repair Therapies.. Woodhead Publishing, pp. 3-38., (10.1533/9780857093301.1.3)
2010
- Enoch, S.et al. 2010. 'Young' oral fibroblasts are geno/phenotypically distinct. Journal of Dental Research 89(12), pp. 1407-1413. (10.1177/0022034510377796)
- Hardwicke, J.et al. 2010. Bioresponsive dextrin-rhEGF conjugates: in vitro evaluation in models relevant to its proposed use as a treatment for chronic wounds. Molecular Pharmaceutics 7(3), pp. 699-707. (10.1021/mp9002656)
- Davies, L. C.et al. 2010. A multipotent neural crest derived progenitor cell population is resident within the oral mucosa lamina propria. Stem Cells and Development 19(6), pp. 819-830. (10.1089/scd.2009.0089)
- Simpson, R. M. L.et al. 2010. Aging fibroblasts resist phenotypic maturation because of impaired hyaluronan-dependent CD44/epidermal growth factor receptor signaling. American Journal of Pathology 176(3), pp. 1215-1228. (10.2353/ajpath.2010.090802)
- Stephens, P. 2010. Development of a cell-based diabetic wound assay. ATLA Alternatives to Laboratory Animals 38(SUPPL.), pp. 45-48.
2009
- Simpson, R. M. L.et al. 2009. Age-related changes in pericellular hyaluronan organization leads to impaired dermal fibroblast to myofibroblast differentiation. American Journal of Pathology 175(5), pp. 1915-1928. (10.2353/ajpath.2009.090045)
- Enoch, S.et al. 2009. Increased oral fibroblast lifespan is telomerase-independent. Journal of Dental Research 88(10), pp. 916-921. (10.1177/0022034509342979)
- Iversen, A.et al. 2009. A proviral role for CpG in cytomegalovirus infection. Journal of Immunology 182(9), pp. 5672-5681. (10.4049/jimmunol.0801268)
- Simpson, R. M.et al. 2009. Age related changes in pericellular hyaluronan leads to impaired dermal fibroblast to myofibroblast differentiation. International Journal of Experimental Pathology 90(2), pp. A128-A129.
- Enoch, S. and Stephens, P. 2009. Scarless healing: Oral mucosa as a scientific model. Wounds UK 5(1), pp. 42-48.
- Cook, H.et al. 2009. Phenotypic differences in wound healing responses are reflected by differences in ECM reorganisation and MMP-2 activation. Journal of Gastroenterology and Hepatology 24, pp. A88-A88. (10.1046/j.1365-2613.2000.0145k.x)
2008
- Wall, I. B.et al. 2008. Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers. Journal of Investigative Dermatology 128(10), pp. 2526-2540. (10.1038/jid.2008.114)
- Hardwicke, J.et al. 2008. Dextrin-rhEGF conjugates as bioresponsive nanomedicines for wound repair. Journal of Controlled Release 130(3), pp. 275-283. (10.1016/j.jconrel.2008.07.023)
- Meran, S.et al. 2008. Hyaluronan facilitates transforming growth factor-β1-mediated fibroblast proliferation. Journal of Biological Chemistry 283(10), pp. 6530-6545. (10.1074/jbc.M704819200)
- Peake, M. A.et al. 2008. Identifying a Gene signature for the wound healing continuum [Abstract]. Wound Repair and Regeneration 16(6), pp. A71. (10.1111/j.1524-475X.2008.00424.x)
- Enoch, S.et al. 2008. The oral mucosa: A model of wound healing with reduced scarring. Oral Surgery 1, pp. 11-21. (10.1111/j.1752-248x.2007.00005.x)
- Mantripragada, K. K.et al. 2008. Telomerase activity is a biomarker for high grade malignant peripheral nerve sheath tumors in neurofibromatosis type I individuals. Genes Chromosomes and Cancer 47(3), pp. 238-246. (10.1002/gcc.20525)
- Meran, S.et al. 2008. Hyaluronan facilitates TGF beta 1 mediated myofibroblastic differentiation [Abstract]. Wound Repair and Regeneration 16(2), pp. A12. (10.1111/j.1524-475X.2008.00371.x)
2007
- Lygoe, K. A.et al. 2007. Role of vitronectin and fibronectin receptors in oral mucosal and dermal myofibroblast differentiation. Biology of the Cell 99(11), pp. 601-614. (10.1042/BC20070008)
- Meran, S.et al. 2007. Involvement of hyaluronan in regulation of fibroblast phenotype. The Journal of Biological Chemistry 282(35), pp. 25687-25697. (10.1074/jbc.M700773200)
- Davies, C. E.et al. 2007. A prospective study of the microbiology of chronic venous leg ulcers to re-evaluate the clinical predictive value of tissue biopsies and swabs. Wound Repair and Regeneration 15(1), pp. 17-22. (10.1111/j.1524-475X.2006.00180.x)
- Stephens, P. and Genever, P. 2007. Non-epithelial oral mucosal progenitor cell populations. Oral Diseases 13(1), pp. 1-10. (10.1111/j.1601-0825.2006.01314.x)
- Andersen, A.et al. 2007. Bacterial profiling using skin grafting, standard culture and molecular bacteriological methods. Journal of Wound Care 16(4), pp. 171-175.
2004
- Moseley, R.et al. 2004. Comparison of oxidative stress biomarker profiles between acute and chronic wound environments. Wound Repair and Regeneration 12(4), pp. 419-429. (10.1111/j.1067-1927.2004.12406.x)
- Davies, C. E.et al. 2004. Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for analysis of the microfloras of healing and non-healing chronic venous leg ulcers. Journal of Clinical Microbiology 42(8), pp. 3549-3557. (10.1128/JCM.42.8.3549-3557.2004)
- Stephens, P.et al. 2004. Crosslinking and G-protein functions of transglutaminase 2 contribute differentially to fibroblast wound healing responses. Journal of cell science 117(15), pp. 3389-3403. (10.1242/jcs.01188)
- Enoch, S.et al. 2004. Identifying the molecular and genetic basis for non-healing wounds, scarless healing and scarring: study of human fibroblasts. British Journal of Surgery 91(S1), pp. 82-82.
- Moseley, R.et al. 2004. Extracellular matrix metabolites as potential biomarkers of disease activity in wound fluid: lessons learned from other inflammatory diseases?. British Journal of Dermatology 150(3), pp. 401-413. (10.1111/j.1365-2133.2004.05845.x)
- Moseley, R.et al. 2004. Comparison of oxidative stress biomarker profiles between acute and chronic wound environments. Wound Repair and Regeneration 12(4), pp. 419-429. (10.1111/j.1067-1927.2004.12406.x)
2003
- Hill, K. E.et al. 2003. Molecular analysis of the microflora in chronic venous leg ulceration. Journal of Medical Microbiology 52(4), pp. 365-369. (10.1099/jmm.0.05030-0)
- Stephens, P.et al. 2003. Anaerobic cocci populating the deep tissues of chronic wounds impair cellular wound healing responses in vitro. British Journal Of Dermatology 148(3), pp. 456-466. (10.1046/j.1365-2133.2003.05232.x)
- Stephens, P.et al. 2003. An analysis of replicative senescence in dermal fibroblasts derived from chronic leg wounds predicts that telomerase therapy would fail to reverse their disease-specific cellular and proteolytic phenotype. Experimental Cell Research 283(1), pp. 22-35. (10.1016/S0014-4827(02)00021-6)
- White, P.et al. 2003. Deletion of the homeobox gene PRX-2 affects fetal but not adult fibroblast wound healing responses. Journal Of Investigative Dermatology 120(1), pp. 135-144. (10.1046/j.1523-1747.2003.12015.x)
2002
- Hill, K. E.et al. 2002. Heterogeneity within the gram-positive anaerobic cocci demonstrated by analysis of 16S-23S intergenic ribosomal RNA polymorphisms. Journal of Medical Microbiology 51(11), pp. 949-957.
- Wall, I. B.et al. 2002. Potential role of anaerobic cocci in impaired human wound healing. Wound Repair and Regeneration 10(6), pp. 346-353. (10.1046/j.1524-475X.2002.t01-1-10602.x)
- Stephens, P. and Thomas, D. W. 2002. The cellular proliferative phase of the wound repair process. Journal of Wound Care 11(7), pp. 253-261. (10.12968/jowc.2002.11.7.26421)
2001
- Davies, C. E.et al. 2001. Use of molecular techniques to study microbial diversity in the skin: Chronic wounds reevaluated. Wound Repair and Regeneration 9(5), pp. 332-340. (10.1046/j.1524-475x.2001.00332.x)
- Stephens, P.et al. 2001. Skin and oral fibroblasts exhibit phenotypic differences in extracellular matrix reorganization and matrix metalloproteinase activity. British Journal Of Dermatology 144(2), pp. 229-237. (10.1046/j.1365-2133.2001.04006.x)
- Stephens, P.et al. 2001. Phenotypic variation in the production of bioactive hepatocyte growth factor/scatter factor by oral mucosal and skin fibroblasts. Wound Repair and Regeneration 9(1), pp. 34-43. (10.1046/j.1524-475x.2001.00034.x)
2000
- Cook, H.et al. 2000. Defective Extracellular Matrix Reorganization by Chronic Wound Fibroblasts is Associated with Alterations in TIMP-1, TIMP-2, and MMP-2 Activity. Journal of Investigative Dermatology 115(2), pp. 225-233. (10.1046/j.1523-1747.2000.00044.x)
- Thomas, D. W.et al. 2000. Randomized clinical trial of the effect of semi-occlusive dressings on the microflora and clinical outcome of acute facial wounds. Wound Repair and Regeneration 8(4), pp. 258-263. (10.1046/j.1524-475x.2000.00258.x)
1998
- Stephens, M.et al. 1998. Molecular characterisation of tumour infiltrating lymphocytes in oral squamous cell carcinoma. Cancer Immunology, Immunotherapy 46(1), pp. 34-40. (10.1007/s002620050457)
1997
- Lim, S. H.et al. 1997. Molecular analysis of T cell receptor beta variability in a patient with orofacial granulomatosis. Gut 40(5), pp. 683-686. (10.1136/gut.40.5.683)
- Thomas, D. W.et al. 1997. T-cell receptor Vbeta usage by lesional lymphocytes in oral lichen planus. Journal of Oral Pathology & Medicine 26(3), pp. 105-109. (10.1111/j.1600-0714.1997.tb00031.x)
- al-Khateeb, T.et al. 1997. An investigation of preferential fibroblast wound repopulation using a novel in vitro wound model. Journal of Periodontology 68(11), pp. 1063-1069. (10.1902/jop.1997.68.11.1063)
- Stephens, P.et al. 1997. Integrin receptor involvement in actin cable formation in an in vitro model of events associated with wound contraction. The International Journal of Biochemistry & Cell Biology 29(1), pp. 121-128. (10.1016/S1357-2725(96)00123-9)
1996
- Stephens, P.et al. 1996. A comparison of the ability of intra-oral and extra-oral fibroblasts to stimulate extracellular matrix reorganization in a model of wound contraction. Journal of Dental Research 75(6), pp. 1358-1364. (10.1177/00220345960750060601)
- Stephens, P.et al. 1996. An investigation of the interaction between alcohol and fibroblasts in wound healing. International Journal of Oral and Maxillofacial Surgery 25(2), pp. 161-164. (10.1016/S0901-5027(96)80065-8)
- Lim, S. H.et al. 1996. T cell receptor Vβ repertoire of tumour-infiltrating lymphocytes in oral squamous-cell carcinoma. Cancer Immunology, Immunotherapy 42(1), pp. 69-70. (10.1007/s002620050253)
Dental BDS (Oral Ecosystems), Biosciences BSc (Tissue Engineering module), CITER Tissue Engineering MSc
Probing the mechanical control of stem cell fate through the development of novel, non-invasive imaging technologies (funded by the EPSRC) The Engineering and Physical Sciences Research Council has jointly awarded Cardiff University and Swansea University £1.5M as part of their recent Novel Technologies for Stem Cell Sciencecall. This is a cross School and cross University project led by Professor Phil Stephens (Dentistry) involving colleagues from the Schools of Biosciences (Dr Paola Borri, Dr Susan Hunter, Dr Stephen Paisey), Chemistry (Dr Eric Tippmann), Dentistry (Dr Alastair Sloan), Medicine (Dr Rachel Errington), Physics (Dr Wolfgang Langbein), the ESRC Centre for the Economic and Social Aspects of Genomics (Dr Neil Stephens), the Cardiff Institute for Tissue Engineering and Repair and from the School of Engineering at Swansea University (Dr Karl Hawkins, Dr Chris Wright, Professor Rhodri Williams). Whilst in our increasing ageing population stem cell science and technology holds a great deal of promise within the context of tissue repair and regeneration, moving this technology to the clinics has been relatively slow due to a number of distinct $acirc; barriers$acirc; . For example, whilst we know a lot about the function and response of stem cells in the laboratory, we know very little about their behaviour in tissues within individuals. A further major barrier has been the inability to accurately track cell lineages and to distinguish them from other cell types within the tissue (i.e. when one cell divides to become two cells are they the same or different? Is this effect the same or different each time the cell divides? Where do they go?). The project will address these issues by bringing together researchers across different scientific disciplines in the physical and life sciences to develop novel technologies for stem cell science. We will develop new ways of non-destructively labelling stem cells by manipulating molecules within the cells so we can follow both their position and their eventual fate (i.e. what do these stem cells turn into?). In order to image the cells we will develop new microscopic techniques that allow us to view these cells in a non-invasive, non-harmful way (unlike current approaches) and we will utilise technologies that will eventually enable us to image these cells deep within patient tissues. Being able to follow these stem cells will allow us to examine the mechanical influence of their surrounding tissue environments. Armed with such knowledge we will mechanically manipulate the surrounding environment to direct stem cells into our tissue of choice in order to deliver custom designed tissues on demand (either within the laboratory or eventually within a patient). Overall, our ultimate aim is to develop new tools to allow us to investigate and control stem cell biology in order to realise the true clinical potential of these cells. Oral mucosal progenitor cells, preferential wound healing outcome and immunomodulation (funded by the WORD) Over the past decade we have reported that patient matched oral mucosal and skin fibroblasts have distinct phenotypic and genotypic differences which result in the preferential healing response seen within the oral mucosa. These findings support the idea that a progenitor cell (PC) population may be resident specifically within the oral mucosal lamina propria (OMLP) and it is this cell population that may contribute to the preferential healing response seen on wounding of this tissue. Importantly, isolation of a PC population from the OMLP would offer distinct advantages for therapeutic applications, providing a site of biopsy that is easily accessible and minimally invasive, with rapid healing and no/minimal resultant scar formation for the patient. We have now demonstrated the existence of this novel, neural crest-derived PC population resident within the lamina propria of the oral mucosa (Davies et al., 2010 in press; UK patent applications GB810841.7, GB0811865.5, GB0820012.3 & International patent application GB09/001443). Single cell clones isolated from these tissues have been rapidly expanded in vitro, are neural crest-derived and are multipotent (mesenchymal, neuronal and glial lineages). We are now investigating their immunomodulatory capacities. The development of in vitro alternatives to animal wound model systems (funded by the NC3Rs) Chronic wounds, such as diabetic foot ulcers, result in impaired wound healing in 3-5% of the population over the age of 65. However, despite the increasing financial burden of these diabetic wounds there is, at present, no suitable diabetic chronic wound animal model. Therefore, in the light of the concerns and limitations of animal models and human testing, this NC3Rs Grant application will develop a stable, reproducible, in vitro diabetic wound model system. This will permit rapid, low cost testing of materials, reagents and drugs in order to reduce unnecessary animal experimentation. We have already demonstrated that venous leg ulcer derived fibroblasts (CWF) and diabetic foot ulcer fibroblasts (DF) are phenotypically distinct from patient-matched normal fibroblasts (NF). We have also immortalised both the NF and DF (by retroviral insertion of the human telomerase) to create stable, disease-specific cell lines and within these cells have identified disease specific marker genes by microarray analysis. The aim of this NC3Rs Grant application will be to extend these initial studies/findings and, using a virus integration approach, stably transduce our immortalised disease cells with fluorescent disease marker gene reporter constructs. This will give us a robust, cell-based reporter system, enabling automated testing and pre-screening of reagents which may ameliorate the diabetic wound disease state. It is anticipated that at the end of the funding we will be close to commercialising the bioassay and developing a high-throughput screening system, which will reduce the amount of unnecessary animal studies undertaken with respect to wound product/materials testing. We believe that the development of such an in vitro diabetic wound model will represent an important and unique resource for wound healing researchers Worldwide. Current Funding EPSRC (2010-2013; multi-centre grant) Previous funding: Wellcome Trust Value in People award (2007-2008)Ongoing Research Projects
Wales Office of Research and Development for Health and Social Care (2010-2011)
NC3Rs (2009-2011)
The Osteology Foundation (2007-2008)
Research into Ageing (2006-2009)
EPSRC (2006-2009; multi-centre grant)
NC3Rs (2006-2009)
Wales Office of Research and Development for Health and Social Care (2006-2007)
The Dr Hadwen trust (2004-2007)
Diabetes UK (2004-2007)
NC3Rs/LASA Small Award Scheme (2007)
Royal College of Surgeons (Edin) (2004-2005)
Johnson & Johnson (2003-2004)
Royal College of Surgeons (Edin) (2003-2004)
British Association of Oral and Maxillofacial Surgeons (2003-2004)
FMC, Norway (2002-2004)
Johnson & Johnson (2001-2003)
Johnson & Johnson (2001-2003)
Astratech, Sweden (2001-2002)
Wales Office of Research and Development for Health and Social Care (2001-2002)
Wales Office of Research and Development for Health and Social Care (2001-2002)
Research Into Ageing - Dyne Steel Wound Healing Research Fellowship (2000-2002)
Research Into Ageing (1999-2000)
Oral and Dental Research Trust, The Colgate Research Award (1999)
National Institutes of Health, USA (1998-2003)
Veterans Affairs Medical Center, USA (1998-2001)
Beiersdorf AG, Germany (1996-1998)
Oral and Dental Research Trust, The Colgate Research Award (1996)