Flow cytometer – cell sorter BD FACS Aria
| Brand/model | BD FACS Aria |
|---|---|
| Manylion | The flow cytometer has three lasers (405nm, 488nm and 633nm) and can be used for cell analysis or cell sorting into tubes, tissue culture plates or slides. |
| Cyfleuster | Stem Cell and Flow Cytometry Unit |
| Ysgol | Ysgol y Biowyddorau |
Availability can be checked with the online Aria booking form. Users will be charged from the start of the booking slot. A minimum of 24 hours notice must be given for cancellations to avoid penalty charges. The FACS machines are available for booking between 09:00 and 17:00 and the facility can only be accessed with a swipe card and key.
Internal users
- Training: N.A.
- Setup: £25
- Without operator: £25
- With operator: £50
- Cancellation fee (less than 24 hour notice): £25
- Offline analysis: £25
External users
| External (academic) | External (industry) | |
|---|---|---|
| Training | N.A. | N.A. |
| Setup charge | £35 | £50 |
| With operator | £75 | £100 |
| Cancellation fee (less than 24 hour notice) | £35 | £50 |
| Off-line analysis | £35 | £50 |
This machine can only be used with the assistance of the facility supervisor.
User guides
Consumables
It is important that cells are filtered before using the FACS machines in order to avoid cell clumps sticking to the interior of the instruments. Below is a list of filters that are currently used.
| Supplier | Description | Pack size | Price |
|---|---|---|---|
| BD | Sterile 40 m m cell strainers (50ml tubes) | 50 | £30 |
| Celltrics | Sterile 30 m m cells strainers (15ml tubes) | 50 | £60 |
| Celltrics | Non-sterile 30 m m cells strainers (15ml tubes) | 250 | £60 |
This machine can only be used for sterile or fixed cell samples.
Cysylltwch
Dr Kirsty Richardson
- richardsonk1@cardiff.ac.uk
- +44 (0)29 2087 5158
Lleoliad
W/4.02
Sir Martin Evans Building
Rhodfa'r Amgueddfa
CF10 3AX
Sir Martin Evans Building
Rhodfa'r Amgueddfa
CF10 3AX
Oriau agor
09:00 - 17:00