Dr Sarah Youde

Dr Sarah Youde

Research Technician (tissue culture)

School of Dentistry

Mae'r cynnwys hwn ar gael yn Saesneg yn unig.

I joined the Dental School in 2006, initially as a research technician with the Mineralised Tissue group. I took over running the Tissue Culture facilities in 2008.  

I am responsible for supporting tissue culture within the department. This includes carrying out project work, training new comers to the department in both theory and practical work and maintaining the facilities to ensure everyone has access to a high quality facility and ordering consumables.

Hours of work

  • Monday, 8.30am - 3pm
  • Wednesday, 8.30am - 3pm
  • Thursday, 8.30am - 2.30pm

I hold a PhD from the University Of Wales College Of Medicine, Dept of Endocrinology. The hypothesis of the thesis was whether cross reactivity between heat shock protein 70 and thyroid peroxidase could cause autoimmune thyroid disease due to mistaken identity.

Following on from this, I worked as a postdoc with Prof Sir L Borysiewicz (now Vice Chancellor of Cambridge University) and Dr Steve Man. My project involved investigating T cell response to human papilloma virus (HPV) and focused on identifying cytotoxic T lymphocyte (CTL) epitopes from HPV.  

An epitope that had previously been shown to generate CTL from healthy controls and patients with cervical cancer was studied and was found to be expressed by cervical carcinoma cells suggesting a therapeutic use.

Experience includes:

  • Cell culture of a wide range of cell types, both primary and established cell lines
  • Transformation of cell lines with viral vectors
  • FACS analysis and cell sorting
  • 3H-thymidine incorporation assays to measure cell growth
  • Chromium release assays
  • MACS
  • Cytometric bead array
  • ELISA and ELISpot
  • SDS-PAGE and Western Blot
  • Immunoprecipitation
  • Immunohistochemistry