Studying human neuropsychiatric disease in DLG2 deficient human neurons
DLG2 has been found to be associated with development of schizophrenia. It has received little attention so far regarding its role in neural development and in the context of human neurons.
Currently the study tries to understand DLG2’s role during neural development and in mature neurons using a variety of techniques such as stem cell culture, neuronal differentiation, neuronal conversion using lenti viral delivery, genetic engineering using CRISPR/Cas9, migration assay, immunocytochemistry, immunohistochemistry, western blot, qRT-PCR, flow cytometry, calcium imaging, single cell patch clamping, microelectrode array and fluorescent in situ hybridisation.
We also have DLG2 knockout mouse and will use this model as well if needed in the study.