Spatial epidemiology in sub-Saharan African wildlife: schistosomes of Cape Buffalo

This research project is in competition for funding with one or more projects available across the NERC GW4 Doctoral Training Partnership (DTP). Usually the projects which receive the best applicants will be awarded the funding. Find out more information about the DTP and how to apply.

Application deadline: 7 January 2019

Start date: October 2019

DTP research theme: Living world

Background

Animal distributions, including disease vectors and parasites, are influenced by environmental features, which can either facilitate or restrict their movement across the landscape. Local adaptation, dispersal behaviour and successful reproduction leaves a genomic footprint, allowing organisms to be tracked through space and time.

Such data allow us to determine the drivers of disease emergence and spread, which can translate into more effective preventative and/or control measures to mitigate outbreaks. Schistosomiasis is a neglected tropical disease of great medical and veterinary importance caused by a parasitic trematode. The parasites have a complex transmission cycle involving specific aquatic freshwater snails and a mammalian host.

Focusing on the Cape buffalo (Syncerus caffer caffer), a keystone species critical to the health of savannah grassland ecosystems, this studentship will involve fieldwork, species identification (snail and parasites) and landscape genomics to identify the drivers of schistosomiasis dynamics in the iconic Kruger National Park (KNP), South Africa.

Project aims and methods

You will:

  • collect, identify and determine the distribution of Buffalo schistosome parasites within the Kruger National Park (WP1);
  • identify and assess diversity of Bulinus snail intermediate hosts (WP2);
  • assess the “genetic health and population structure of Cape buffalos (WP3);
  • correlate landscape variables with genetic diversity (WP4).

To achieve these aims, WP1-2 will involve morphological identification and molecular metabarcoding. For WP3, you will apply double-digest restriction-site associated DNA sequencing to obtain genome-wide SNP loci to resolve fine scale population structure in buffalo.

WP4 will deploy a landscape genomics approach incorporating GIS resistance maps for each environmental variable. Using R statistical packages, resistance distance will be correlated with buffalo genetic distance in order to identify variables that are either positivity or negatively correlated with gene flow. Lastly, current maps will be generated in CIRCUITSCAPE to identify areas within the landscape of high and low connectivity that can inform disease spread and predictions of outbreaks.

This project will provide a model for future landscape genomics of zoonotic disease vectors making a significant contribution to the field of molecular epidemiology.

Candidate

The successful candidate will demonstrate experience in, and willingness to, undertake international fieldwork, specifically in South Africa. A strong background in population genetics and metabarcoding, or related molecular approaches, is desirable, and/or experience in statistical modelling and Geographic Information Systems (GIS).

Case or Collaborative Partner

You will join the NERC FRESH cohort of students, having unique accessto related freshwater training.

Phoenix provides the student with the opportunity to meet other environmental researchers insub-Saharan African, who aim to integrate medical and ecological interventions to reduce disease burden.

SANparks provides logistics (safety incl. guides, site access, vehicles) access to the iconic KrugerNational Park. Permits have been approved by SANParks ethics committee.

In addition, the Natural History Museum (NHM) will integrate you with a world class schistosomiasis research group, which hosts the largest global repository of schistosomiasis-related specimens.

Training

You will attend relevant advanced training courses (incl. FRESH field skills, and DTP statistical analysis in R), and be encouraged to join relevant scientific societies, such as the British Society of Parasitology, and present your data at conferences.

You will be trained by Cable (parasitology), Russo (field techniques, molecular sampling, landscape genomics), Bruford (population genetics), Webster (snail and schistosome identification) and generic training (writing and oral presentation skills, statistics). Finally, you will be supported by the Cardiff Doctoral Academy (team building, data and project management).

References and background reading

  • Beechler BR et al. 2017. Host immunity, nutrition and coinfection alter longitudinal infection patterns of schistosomes in a free ranging African buffalo population. PLoS Neglected Tropical Diseases 11(12): e0006122.
  • Biek R & Real LA. 2010. The landscape genetic of infectious disease emergence and spread. Molecular Ecology 19, 3515-3531. Caron A et al. 2016. African buffalo movement and zoonotic disease risk across transfrontier conservation areas, southern Africa. Emerging Infectious Diseases 22, 277-280.
  • Hemming-Schroeder E et al. 2018. Landscape Genetics: a toolbox for studying vector-borne diseases. Frontiers in Ecology and Evolution 6:21.
  • Hughes G et al. 2017. Modeling the spatial distribution of African buffalo (Syncerus caffer) in the Kruger National Park, South Africa. PLoS ONE 12(9): e0182903.
  • Ma T et al. 2018. Walking in a heterogeneous landscape: dispersal, gene-flow and conservation implications for the giant panda in the Qinling Mountains. Evolutionary Applications 00:1-14.
  • Russo IM et al. 2016. Landscape determinants of fine-scale genetic structure of a small rodent in a heterogeneous landscape (Hluhluwe-iMfolozi Park, South Africa). Scientific Reports 6: 29168.
  • Schwabl P et al. 2018. Prediction and prevention of parasitic diseases using a landscape genomics framework. Trends in Parasitology 33, 264-274.

Supervisors

Professor Joanne Cable

Professor Jo Cable

Professor

Email:
cablej@cardiff.ac.uk
Telephone:
+44 (0)29 2087 6022
Dr Isa-Rita Russo

Dr Isa-Rita Russo

Research Associate

Email:
russoim@cardiff.ac.uk
Telephone:
+44 (0)29 2087 5073
Professor Michael Bruford

Professor Mike Bruford

Co-director Sustainable Places Research Institute

Email:
brufordmw@cardiff.ac.uk
Telephone:
+44 (0)29 2087 4312

Co-supervisors

Dr Bonnie Webster, Natural History Museum, London, Parasites and Vectors Division

Programme information

For programme structure, entry requirements and how to apply, visit the programme.

View programme
Find out more about Postgraduate study at our Open Day on 21 November 2018.

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