![Chris Von Ruhland BSc MPhil PhD](https://profiles-cardiff.imgix.net/attachments/2631?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Dr Chris Von Ruhland
(he/him)
BSc MPhil PhD
Facility Lead (Electron and Light Microscopy), Central Biotechnology Services
- VonRuhlandCJ@cardiff.ac.uk
- +44 29207 42132
- Main Hospital Building, Room 1LB5 71, University Hospital of Wales, Heath Park, Cardiff, CF14 4XN
Overview
I provide a biological electron and light microscopy service, covering all aspects of sample preparation, and advise on experimental design to researchers at Cardiff University, other academic institutions, the NHS, and industry. Since beginning my microscopy career in 1997, I have examined and analysed a wide variety of samples, including:
- Tissue such as kidney, brain, skin, fat and bone
- Cultured and free living cells
- Extracellular vesicles and viruses
- Mineral fibres and nanoparticles
My technological research interests include:
- Improving tissue processing techniques and developing novel approaches to sample preparation.
- Enhancing the sensitivity of immunohistochemical staining using marker amplification, and the development of novel markers with a view to automated single molecule detection at the light microscopic level
- Designing novel embedding resins and staining techniques for use in SEM tomography
Publication
2024
- Rahbari, R., Francis, L., Guy, O. J., Sharma, S., Von Ruhland, C. and Xia, Z. 2024. Microneedle-assisted transfersomes as a transdermal delivery system for aspirin. Pharmaceutics 16(1), article number: 57. (10.3390/pharmaceutics16010057)
2023
- Casella, C. et al. 2023. Differences in white matter detected by ex vivo 9.4T MRI are associated with axonal changes in the R6/1 model of Huntington’s Disease. [Online]. BioRXiv: BioRXiv. (10.1101/2023.10.02.560424) Available at: https://doi.org/10.1101/2023.10.02.560424
- Powell, L. C. et al. 2023. Alginate oligosaccharides enhance the antifungal activity of nystatin against candidal biofilms. Frontiers in Cellular and Infection Microbiology 13, article number: 1122340. (10.3389/fcimb.2023.1122340)
2022
- Kanagasingam, S., von Ruhland, C., Welbury, R. and Singhrao, S. K. 2022. Ex vivo detection of Amyloid-β in naturally formed oral biofilm. Journal of Alzheimer's Disease Reports 6(1), pp. 757-773. (10.3233/ADR-220076)
- Kanagasingam, S., von Ruhland, C., Welbury, R. and Singhrao, S. K. 2022. Antimicrobial, polarizing light, and paired helical filament properties of fragmented tau peptides of selected putative gingipains. Journal of Alzheimer's Disease 89(4) (10.3233/JAD-220486)
- Kanagasingam, S., von Ruhland, C., Welbury, R., Chukkapalli, S. S. and Singhrao, S. K. 2022. Porphyromonas gingivalis conditioned medium induces amyloidogenic processing of the amyloid-β protein precursor upon in vitro infection of sh-sy5y cells. Journal of Alzheimer's Disease Reports 6(1), pp. 577-587. (10.3233/ADR-220029)
- Tatovic, D. et al. 2022. Safety of the use of gold nanoparticles conjugated with proinsulin peptide and administered by hollow microneedles as an immunotherapy in Type 1 diabetes. Immunotherapy Advances 2(1), article number: ltac002. (10.1093/immadv/ltac002)
2021
- Ball, P., Hobbs, R., Anderson, S., Thompson, E., Gwenin, V., Von Ruhland, C. and Gwenin, C. 2021. The YfkO nitroreductase from bacillus licheniformis on gold-coated superparamagnetic nanoparticles: towards a novel directed enzyme prodrug therapy approach. Pharmaceutics 13(4), article number: 517. (10.3390/pharmaceutics13040517)
2020
- Zhang, L. et al. 2020. Distinctive features of orbital adipose tissue (OAT) in Graves' orbitopathy. International Journal of Molecular Sciences 21(23), article number: 9145. (10.3390/ijms21239145)
2019
- Kelly, S. E. and von Ruhland, C. J. 2019. The use of metal-catalyzed oxidation to suppress background staining caused by marker amplification reagents and the effects of this oxidation on the stability of antibody-antigen complexes in immunohistochemistry. Biotechnic and Histochemistry 94(130), pp. 36-41. (10.1080/10520295.2018.1493223)
2018
- von Ruhland, C. 2018. Application of an optimized marker amplification protocol in immunohistochemistry — a valuable tool for visualizing antigenic sites of low signal strength or sparse distribution. Biotechnic and Histochemistry 93(7), pp. 478-484. (10.1080/10520295.2018.1427792)
2017
- Welton, J. L., Loveless, S., Stone, T., Von Ruhland, C., Robertson, N. and Clayton, A. 2017. Cerebrospinal fluid extracellular vesicle enrichment for protein biomarker discovery in neurological disease; multiple sclerosis. Journal of Extracellular Vesicles 6(1), article number: 1369805. (10.1080/20013078.2017.1369805)
- Dhanjal, T. S. et al. 2017. Massive accumulation of myofibroblasts in the critical isthmus is associated with ventricular tachycardia inducibility in post-infarct swine heart. Journal of the American College of Cardiology: Clinical Electrophysiology 3(7), pp. 703-714. (10.1016/j.jacep.2016.11.010)
- Xia, Z. et al. 2017. Nano-analyses of wear particles from metal-on-metal and non-metal-on-metal dual modular neck hip arthroplasty. Nanomedicine: Nanotechnology, Biology and Medicine 13(3), pp. 1205-127. (10.1016/j.nano.2016.11.003)
- Brewis, I., Watkinson, M. and Von Ruhland, C. 2017. Heads you win, tails you lose: filtration processing for the microscopical examination of sperm heads. Journal of Microscopy 265(3), pp. 335-340. (10.1111/jmi.12505)
2016
- Teoh, K. H. et al. 2016. Metallosis following implantation of magnetically controlled growing rods in the treatment of scoliosis: a case series. The Bone & Joint Journal 98-B(12), pp. 1662-1667. (10.1302/0301-620X.98B12.38061)
- Dhanjal, T., Lellouche, N., Von Ruhland, C. J., Edwards, D. H., George, C. and Williams, A. 2016. LAVA sites are histologically closely related to critical isthmus sites in post-infarct ventricular tachycardia. Presented at: Heart Rhythm 2016 - 37th Annual Scientific Sessions, San Francisco, CA, USA, 4 May 2016.
- Dhanjal, T., Lellouche, N., Von Ruhland, C., Edwards, D., George, C. and Williams, A. 2016. Increased myofibroblast densities are specific to critical isthmus sites in post-infarct re-entrant ventricular tachycardia. Presented at: Heart Rhythm Society, San Francisco, May 2016.
2015
- Connolly, K. D. et al. 2015. Characterisation of adipocyte-derived extracellular vesicles released pre- and post-adipogenesis. Journal of Extracellular Vesicles 4(s1), article number: 29159. (10.3402/jev.v4.29159)
2014
- Von Ruhland, C., Scully, R. and Griffiths, D. 2014. The anatomical variation in normal human glomerular basement membrane thickness: preliminary observations. Virchovs Archiv 465, pp. S330., article number: PS-21-004. (10.1007/s00428-014-1618-2)
- Von Ruhland, C., Scully, R., Morgan, J. and Griffiths, D. 2014. The effects of tissue processing on glomerular basement membrane thickness: a multicentre study. Virchows Archiv 465(S1), pp. S36-S37. (10.1007/s00428-014-1618-2)
2012
- Williams, P. . A., Piechota, M., Von Ruhland, C. J., Taylor, E., Morgan, J. E. and Votruba, M. 2012. Opa1 is essential for retinal ganglion cell synaptic architecture and connectivity. Brain 135(2), pp. 493-505. (10.1093/brain/awr330)
2011
- von Ruhland, C. J. 2011. The molecular basis of modern marker chemistry. PhD Thesis, Cardiff University.
2010
- Von Ruhland, C. J. and Hobot, J. A. 2010. LR White sections as slot grid support films for transmission electron microscopy. Journal of Microscopy 240(2), pp. 130-134. (10.1111/j.1365-2818.2010.03397.x)
- Von Ruhland, C. J. and Jasani, B. 2010. The amplification of polymerized diaminobenzidine with physical developers: sensitizing effects of transition metal salts and sulphide. Journal of Microscopy 238(2), pp. 111-122. (10.1111/j.1365-2818.2009.03334.x)
2009
- Bonnet, C., Aldred, M., Von Ruhland, C. J., Harris, R., Sandford, R. and Cheadle, J. P. 2009. Defects in cell polarity underlie TSC and ADPKD-associated cystogenesis. Human Molecular Genetics 18(12), pp. 2166-2176. (10.1093/hmg/ddp149)
- Edwards, K., Griffiths, D., Morgan, J., Pitman, R. and Von Ruhland, C. 2009. Can the choice of intermediate solvent or resin affect glomerular basement membrane thickness?. Nephrology Dialysis Transplantation 24(2), pp. 400-403. (10.1093/ndt/gfn504)
2008
- Hallett, M. . B., Von Ruhland, C. J. and Dewitt, S. 2008. Chemotaxis and the cell surface-area problem [Letter]. Nature Reviews Molecular Cell Biology 9(8), pp. 662. (10.1038/nrm2419-c1)
- Kafienah, W. et al. 2008. Lumican inhibits collagen deposition in tissue engineered cartilage. Matrix Biology 27(6), pp. 526-534. (10.1016/j.matbio.2008.04.002)
2006
- Satchell, S. et al. 2006. Conditionally immortalized human glomerular endothelial cells expressing fenestrations in response to VEGF. Kidney International 69(9), pp. 1633-1640. (10.1038/sj.ki.5000277)
- Edwards, K., von Ruhland, C., Morgan, M. and Griffiths, D. 2006. The effects of intermediate solvents and resins on glomerular basement membrane (GBM) thickness. Presented at: 190th Meeting of the Pathological Society of Great Britain and Ireland, Manchester, ENGLAND, 04-07/07/2006.
2005
- Baker, G. R., Mazziotti, G., Von Ruhland, C. J. and Ludgate, M. E. 2005. Reevaluating thyrotropin receptor-induced mouse models of Graves' disease and ophthalmopathy. Endocrinology 146(2), pp. 835-844. (10.1210/en.2004-1015)
2004
- Von Ruhland, C. J., Campbell, L., Gumbleton, M., Jasani, B. and Newman, G. R. 2004. Immunolocalization of caveolin-1 in rat and human mesothelium. Journal of Histochemistry & Cytochemistry 52(11), pp. 1415-1425. (10.1369/jhc.4A6334.2004)
- Butchart, L., von Ruhland, C., Newman, G., Gumbleton, M., Jones, K., Campbell, L. and Griffiths, D. 2004. Distribution of caveolin 1 in the human glomerulus. Presented at: 186th Meeting of the Pathological Society of Great Britain and Ireland/Dutch Pathological Society, Vrije Univ Med Ctr, Dept Pathol, Amsterdam, NETHERLANDS, 06-09/07/2004.
2003
- Williams, J. D., Craig, K. J., Von Ruhland, C. J., Topley, N. and Williams, G. T. 2003. The natural course of peritoneal membrane biology during peritoneal dialysis. Kidney International 64(s88), pp. SS43-SS49. (10.1046/j.1523-1755.2003.08805.x)
- Von Ruhland, C., Newman, G., Topley, N. and Williams, J. D. 2003. Can artifact mimic the pathology of the peritoneal mesothelium?. Peritoneal Dialysis International 23, pp. 428-433.
2002
- Williams, J. D. et al. 2002. Morphologic changes in the peritoneal membrane of patients with renal disease. Journal of the American Society of Nephrology 13(2), pp. 470-479.
2001
- Von Ruhland, C. and Newman, G. R. 2001. A cytochemical method for visualising blood vessels selectively in semithin LR White sections. Histochemical Journal 33(7), pp. 433-435.
1999
- Campbell, L., Hollins, A. J., Al-Eid, A., Newman, G. R., Von Ruhland, C. J. and Gumbleton, M. 1999. Caveolin-1 expression and caveolae biogenesis during cell transdifferentiation in lung alveolar epithelial primary cultures. Biochemical and Biophysical Research Communications 262(3), pp. 744-751. (10.1006/bbrc.1999.1280)
1997
- Page, R., Morris, C., Williams, J., Von Ruhland, C. and Malik, A. 1997. Isolation of diabetes-associated kidney genes using differential display. Biochemical and Biophysical Research Communications 232(1), pp. 49-53. (10.1006/bbrc.1997.6224)
1994
- Ferguson, C., Von Ruhland, C., Shesthra, B., Griffin, P., Moore, R. H. and Salaman, J. R. 1994. Enalapril and cyclosporin in renal transplant patients and rats. Transplantation Proceedings 26, pp. 2600-2601.
1993
- Ferguson, C., Von Ruhland, C., J, P. D., R, G. D. F. and R, S. J. 1993. Low-dose cyclosporin nephrotoxicity in the rat. Nephrology Dialysis Transplantation 8(11), pp. 1254-1259. (10.1093/ndt/8.11.1259)
1992
- Ferguson, C., Von Ruhland, C., Parry-Jones, D., Williams, J. and Salaman, J. 1992. The effects of niphedipine on cyclosporine nephrotoxicity in rats. Presented at: Proceedings of the 5th Congress of the European Society for Organ Transplantation, Maastricht, Netherlands, 7-10 Oct 1991Transplant International Official Journal of the European Society for Organ Transplantation, Vol. 5. Vol. S1. Springer pp. S490-S493.
Articles
- Rahbari, R., Francis, L., Guy, O. J., Sharma, S., Von Ruhland, C. and Xia, Z. 2024. Microneedle-assisted transfersomes as a transdermal delivery system for aspirin. Pharmaceutics 16(1), article number: 57. (10.3390/pharmaceutics16010057)
- Powell, L. C. et al. 2023. Alginate oligosaccharides enhance the antifungal activity of nystatin against candidal biofilms. Frontiers in Cellular and Infection Microbiology 13, article number: 1122340. (10.3389/fcimb.2023.1122340)
- Kanagasingam, S., von Ruhland, C., Welbury, R. and Singhrao, S. K. 2022. Ex vivo detection of Amyloid-β in naturally formed oral biofilm. Journal of Alzheimer's Disease Reports 6(1), pp. 757-773. (10.3233/ADR-220076)
- Kanagasingam, S., von Ruhland, C., Welbury, R. and Singhrao, S. K. 2022. Antimicrobial, polarizing light, and paired helical filament properties of fragmented tau peptides of selected putative gingipains. Journal of Alzheimer's Disease 89(4) (10.3233/JAD-220486)
- Kanagasingam, S., von Ruhland, C., Welbury, R., Chukkapalli, S. S. and Singhrao, S. K. 2022. Porphyromonas gingivalis conditioned medium induces amyloidogenic processing of the amyloid-β protein precursor upon in vitro infection of sh-sy5y cells. Journal of Alzheimer's Disease Reports 6(1), pp. 577-587. (10.3233/ADR-220029)
- Tatovic, D. et al. 2022. Safety of the use of gold nanoparticles conjugated with proinsulin peptide and administered by hollow microneedles as an immunotherapy in Type 1 diabetes. Immunotherapy Advances 2(1), article number: ltac002. (10.1093/immadv/ltac002)
- Ball, P., Hobbs, R., Anderson, S., Thompson, E., Gwenin, V., Von Ruhland, C. and Gwenin, C. 2021. The YfkO nitroreductase from bacillus licheniformis on gold-coated superparamagnetic nanoparticles: towards a novel directed enzyme prodrug therapy approach. Pharmaceutics 13(4), article number: 517. (10.3390/pharmaceutics13040517)
- Zhang, L. et al. 2020. Distinctive features of orbital adipose tissue (OAT) in Graves' orbitopathy. International Journal of Molecular Sciences 21(23), article number: 9145. (10.3390/ijms21239145)
- Kelly, S. E. and von Ruhland, C. J. 2019. The use of metal-catalyzed oxidation to suppress background staining caused by marker amplification reagents and the effects of this oxidation on the stability of antibody-antigen complexes in immunohistochemistry. Biotechnic and Histochemistry 94(130), pp. 36-41. (10.1080/10520295.2018.1493223)
- von Ruhland, C. 2018. Application of an optimized marker amplification protocol in immunohistochemistry — a valuable tool for visualizing antigenic sites of low signal strength or sparse distribution. Biotechnic and Histochemistry 93(7), pp. 478-484. (10.1080/10520295.2018.1427792)
- Welton, J. L., Loveless, S., Stone, T., Von Ruhland, C., Robertson, N. and Clayton, A. 2017. Cerebrospinal fluid extracellular vesicle enrichment for protein biomarker discovery in neurological disease; multiple sclerosis. Journal of Extracellular Vesicles 6(1), article number: 1369805. (10.1080/20013078.2017.1369805)
- Dhanjal, T. S. et al. 2017. Massive accumulation of myofibroblasts in the critical isthmus is associated with ventricular tachycardia inducibility in post-infarct swine heart. Journal of the American College of Cardiology: Clinical Electrophysiology 3(7), pp. 703-714. (10.1016/j.jacep.2016.11.010)
- Xia, Z. et al. 2017. Nano-analyses of wear particles from metal-on-metal and non-metal-on-metal dual modular neck hip arthroplasty. Nanomedicine: Nanotechnology, Biology and Medicine 13(3), pp. 1205-127. (10.1016/j.nano.2016.11.003)
- Brewis, I., Watkinson, M. and Von Ruhland, C. 2017. Heads you win, tails you lose: filtration processing for the microscopical examination of sperm heads. Journal of Microscopy 265(3), pp. 335-340. (10.1111/jmi.12505)
- Teoh, K. H. et al. 2016. Metallosis following implantation of magnetically controlled growing rods in the treatment of scoliosis: a case series. The Bone & Joint Journal 98-B(12), pp. 1662-1667. (10.1302/0301-620X.98B12.38061)
- Connolly, K. D. et al. 2015. Characterisation of adipocyte-derived extracellular vesicles released pre- and post-adipogenesis. Journal of Extracellular Vesicles 4(s1), article number: 29159. (10.3402/jev.v4.29159)
- Von Ruhland, C., Scully, R. and Griffiths, D. 2014. The anatomical variation in normal human glomerular basement membrane thickness: preliminary observations. Virchovs Archiv 465, pp. S330., article number: PS-21-004. (10.1007/s00428-014-1618-2)
- Von Ruhland, C., Scully, R., Morgan, J. and Griffiths, D. 2014. The effects of tissue processing on glomerular basement membrane thickness: a multicentre study. Virchows Archiv 465(S1), pp. S36-S37. (10.1007/s00428-014-1618-2)
- Williams, P. . A., Piechota, M., Von Ruhland, C. J., Taylor, E., Morgan, J. E. and Votruba, M. 2012. Opa1 is essential for retinal ganglion cell synaptic architecture and connectivity. Brain 135(2), pp. 493-505. (10.1093/brain/awr330)
- Von Ruhland, C. J. and Hobot, J. A. 2010. LR White sections as slot grid support films for transmission electron microscopy. Journal of Microscopy 240(2), pp. 130-134. (10.1111/j.1365-2818.2010.03397.x)
- Von Ruhland, C. J. and Jasani, B. 2010. The amplification of polymerized diaminobenzidine with physical developers: sensitizing effects of transition metal salts and sulphide. Journal of Microscopy 238(2), pp. 111-122. (10.1111/j.1365-2818.2009.03334.x)
- Bonnet, C., Aldred, M., Von Ruhland, C. J., Harris, R., Sandford, R. and Cheadle, J. P. 2009. Defects in cell polarity underlie TSC and ADPKD-associated cystogenesis. Human Molecular Genetics 18(12), pp. 2166-2176. (10.1093/hmg/ddp149)
- Edwards, K., Griffiths, D., Morgan, J., Pitman, R. and Von Ruhland, C. 2009. Can the choice of intermediate solvent or resin affect glomerular basement membrane thickness?. Nephrology Dialysis Transplantation 24(2), pp. 400-403. (10.1093/ndt/gfn504)
- Hallett, M. . B., Von Ruhland, C. J. and Dewitt, S. 2008. Chemotaxis and the cell surface-area problem [Letter]. Nature Reviews Molecular Cell Biology 9(8), pp. 662. (10.1038/nrm2419-c1)
- Kafienah, W. et al. 2008. Lumican inhibits collagen deposition in tissue engineered cartilage. Matrix Biology 27(6), pp. 526-534. (10.1016/j.matbio.2008.04.002)
- Satchell, S. et al. 2006. Conditionally immortalized human glomerular endothelial cells expressing fenestrations in response to VEGF. Kidney International 69(9), pp. 1633-1640. (10.1038/sj.ki.5000277)
- Baker, G. R., Mazziotti, G., Von Ruhland, C. J. and Ludgate, M. E. 2005. Reevaluating thyrotropin receptor-induced mouse models of Graves' disease and ophthalmopathy. Endocrinology 146(2), pp. 835-844. (10.1210/en.2004-1015)
- Von Ruhland, C. J., Campbell, L., Gumbleton, M., Jasani, B. and Newman, G. R. 2004. Immunolocalization of caveolin-1 in rat and human mesothelium. Journal of Histochemistry & Cytochemistry 52(11), pp. 1415-1425. (10.1369/jhc.4A6334.2004)
- Williams, J. D., Craig, K. J., Von Ruhland, C. J., Topley, N. and Williams, G. T. 2003. The natural course of peritoneal membrane biology during peritoneal dialysis. Kidney International 64(s88), pp. SS43-SS49. (10.1046/j.1523-1755.2003.08805.x)
- Von Ruhland, C., Newman, G., Topley, N. and Williams, J. D. 2003. Can artifact mimic the pathology of the peritoneal mesothelium?. Peritoneal Dialysis International 23, pp. 428-433.
- Williams, J. D. et al. 2002. Morphologic changes in the peritoneal membrane of patients with renal disease. Journal of the American Society of Nephrology 13(2), pp. 470-479.
- Von Ruhland, C. and Newman, G. R. 2001. A cytochemical method for visualising blood vessels selectively in semithin LR White sections. Histochemical Journal 33(7), pp. 433-435.
- Campbell, L., Hollins, A. J., Al-Eid, A., Newman, G. R., Von Ruhland, C. J. and Gumbleton, M. 1999. Caveolin-1 expression and caveolae biogenesis during cell transdifferentiation in lung alveolar epithelial primary cultures. Biochemical and Biophysical Research Communications 262(3), pp. 744-751. (10.1006/bbrc.1999.1280)
- Page, R., Morris, C., Williams, J., Von Ruhland, C. and Malik, A. 1997. Isolation of diabetes-associated kidney genes using differential display. Biochemical and Biophysical Research Communications 232(1), pp. 49-53. (10.1006/bbrc.1997.6224)
- Ferguson, C., Von Ruhland, C., Shesthra, B., Griffin, P., Moore, R. H. and Salaman, J. R. 1994. Enalapril and cyclosporin in renal transplant patients and rats. Transplantation Proceedings 26, pp. 2600-2601.
- Ferguson, C., Von Ruhland, C., J, P. D., R, G. D. F. and R, S. J. 1993. Low-dose cyclosporin nephrotoxicity in the rat. Nephrology Dialysis Transplantation 8(11), pp. 1254-1259. (10.1093/ndt/8.11.1259)
Conferences
- Dhanjal, T., Lellouche, N., Von Ruhland, C. J., Edwards, D. H., George, C. and Williams, A. 2016. LAVA sites are histologically closely related to critical isthmus sites in post-infarct ventricular tachycardia. Presented at: Heart Rhythm 2016 - 37th Annual Scientific Sessions, San Francisco, CA, USA, 4 May 2016.
- Dhanjal, T., Lellouche, N., Von Ruhland, C., Edwards, D., George, C. and Williams, A. 2016. Increased myofibroblast densities are specific to critical isthmus sites in post-infarct re-entrant ventricular tachycardia. Presented at: Heart Rhythm Society, San Francisco, May 2016.
- Edwards, K., von Ruhland, C., Morgan, M. and Griffiths, D. 2006. The effects of intermediate solvents and resins on glomerular basement membrane (GBM) thickness. Presented at: 190th Meeting of the Pathological Society of Great Britain and Ireland, Manchester, ENGLAND, 04-07/07/2006.
- Butchart, L., von Ruhland, C., Newman, G., Gumbleton, M., Jones, K., Campbell, L. and Griffiths, D. 2004. Distribution of caveolin 1 in the human glomerulus. Presented at: 186th Meeting of the Pathological Society of Great Britain and Ireland/Dutch Pathological Society, Vrije Univ Med Ctr, Dept Pathol, Amsterdam, NETHERLANDS, 06-09/07/2004.
- Ferguson, C., Von Ruhland, C., Parry-Jones, D., Williams, J. and Salaman, J. 1992. The effects of niphedipine on cyclosporine nephrotoxicity in rats. Presented at: Proceedings of the 5th Congress of the European Society for Organ Transplantation, Maastricht, Netherlands, 7-10 Oct 1991Transplant International Official Journal of the European Society for Organ Transplantation, Vol. 5. Vol. S1. Springer pp. S490-S493.
Thesis
- von Ruhland, C. J. 2011. The molecular basis of modern marker chemistry. PhD Thesis, Cardiff University.
Websites
- Casella, C. et al. 2023. Differences in white matter detected by ex vivo 9.4T MRI are associated with axonal changes in the R6/1 model of Huntington’s Disease. [Online]. BioRXiv: BioRXiv. (10.1101/2023.10.02.560424) Available at: https://doi.org/10.1101/2023.10.02.560424
Biography
Career overview
2016 to present Facility Lead Electron and Light Microscopy Facility, Central Biotechnology Services, College of Biomedical and Life Sciences, Cardiff University
2012 to 2016 Deputy Facility Lead Medical Microscopy Sciences, Central Biotechnology Services, School of Medicine, Cardiff University
2006 to 2012 Deputy Director Medical Microscopy Sciences, Pathology, School of Medicine, Cardiff University
1997 to 2006 Research Technician Electron Microscopy Unit, Pathology, School of Medicine, Cardiff University
1995 to 1997 Research Technician Institute of Nephrology, University of Wales College of Medicine
1985 to 1995 Laboratory Technician Institute of Nephrology, University of Wales College of Medicine
Education and Qualifications
PhD Marker Chemistry, School of Chemistry, Cardiff University
MPhil Cyclosporine Nephrotoxicity, University of Wales College of Medicine
MIBiol Macromolecular Biology, South Glamorgan Institute of Higher Education
BSc (hons) Biological Sciences Plymouth Polytechnic
HD(TEC) Applied Biology North East Surrey College of Technology
Professional memberships
Fellow of the Royal Microscopical Society
Committees and reviewing
Member of the Human Tissue Research Governance Committee
Member of the Technicians Commitment Working Group
Member of the Technical Career Pathway Working Group
Member of the Technical Managers Forum
Engagement
I am engaged in collaborations with the Electron Microscopy Unit, Department of Cellular Pathology, Cardiff and Vale NHS Trust, including:
1. The effects of tissue processing for electron microscopy on the final thickness of the glomerular basement membrane, measurement of which is important in the diagnosis and staging of glomerular diseases.
2.Developing an immunoelectron microscopical assay to positively identify SAR-COV-2 in the tissue of patients with chronic viral infection or 'Long Covid'
Specialisms
- Electron microscopy
- Microscopy
- Histochemistry
- Immunohistochemistry
- Tissue processing