Yr Athro Bruce Caterson

Professor

Ysgol y Biowyddorau

: caterson@cardiff.ac.uk
: +44 (0)29 2087 4595
Fax:: +44 (0)29 2087 4594
: Cardiff School of Biosciences, The Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, Adeilad Syr Martin Evans, Rhodfa'r Amgueddfa, Caerdydd, CF10 3AX

Research Overview

2011

Akhtar, S., Bron, A., Hayes, A. J., Meek, K. M. A. and Caterson, B. 2011. Role of keratan sulphate (sulphated poly -N-acetyllactosamine repeats) in keratoconic cornea, histochemical, and ultrastructural analysis. Graefe's Archive for Clinical and Experimental Ophthalmology 249(3), pp. 413-420. (10.1007/s00417-010-1512-9)
Hayes, A. J., Hughes, C. E., Ralphs, J. R. and Caterson, B. 2011. Chondroitin sulphate sulphation motif expression in the ontogeny of the intervertebral disc. European Cells and Materials 21, pp. 1-14.
Hayes, A. J., Isaacs, M. D., Hughes, C. E., Caterson, B. and Ralphs, J. R. 2011. Collagen fibrillogenesis in the development of the annulus fibrosus of the intervertebral disc. European Cells and Materials 22, pp. 226-241.

2010

Gabriel, N., Innes, J., Caterson, B. and Vaughan-Thomas, A. 2010. Development of an in vitro model of feline cartilage degradation. Journal of Feline Medicine & Surgery 12(8), pp. 614-620. (10.1016/j.jfms.2010.03.007)
Liles, M. et al. 2010. Differential relative sulfation of keratan sulfate glycosaminoglycan in the chick cornea during embryonic development. Investigative Ophthalmology and Visual Science 51(3), pp. 1365-1372. (10.1167/iovs.09-4004)
Bondeson, J., Blom, A. B., Wainwright, S. D., Hughes, C., Caterson, B. and van den Berg, W. B. 2010. The role of synovial macrophages and macrophage-produced mediators in driving inflammatory and destructive responses in osteoarthritis. Arthritis & Rheumatism 62(3), pp. 647-657. (10.1002/art.27290)
Zhang, A. et al. 2010. Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease. Journal of Zhejiang University-Science B 11(3), pp. 200-208. (10.1631/jzus.B0900074)
Hurst, S., Zainal, Z., Caterson, B., Hughes, C. E. and Harwood, J. L. 2010. Dietary fatty acids and arthritis. Prostaglandins Leukotrienes & Essential Fatty Acids 82(4-6), pp. 315-318. (10.1016/j.plefa.2010.02.008)
Yang, B. et al. 2010. In vitrocartilage tissue engineering using cancellous bone matrix gelatin as a biodegradable scaffold. Biomedical Materials 5(4), article number: 45003. (10.1088/1748-6041/5/4/045003)
Caterson, B., Little, C. B., Harwood, J. L., Innes, J. F., Fritsch, D. A., Jewell, D. E. and Schoenherr, W. D. 2010. Method for decreasing cartilage damage in dogs. US20100261793A1 [Patent].
Ralphs, J. R., Caterson, B., Hughes, C. E. and Hayes, A. J. 2010. Chondroitin sulphate sulfation motifs in intervertebral disc development [Abstract]. European Cells and Materials 20(Supp 2), pp. 3.

2009

Zainal, Z., Longman, A. J., Hurst, S., Duggan, K., Caterson, B., Hughes, C. E. and Harwood, J. L. 2009. Relative efficacies of omega-3 polyunsaturated fatty acids in reducing expression of key proteins in a model system for studying osteoarthritis. Osteoarthritis and Cartilage 17(7), pp. 896-905. (10.1016/j.joca.2008.12.009)
Gealy, E. C., Hayes, A. J., Buckwell, R., Young, R. D., Caterson, B., Quantock, A. J. and Ralphs, J. R. 2009. Actin and type I collagen propeptide distribution in the developing chick cornea. Investigative Ophthalmology & Visual Science 50(4), pp. 1653 -1658. (10.1167/iovs.08-2554)
Clarkin, C. E. et al. 2009. Phenotypic plasticity of superficial zone chondrocytes is revealed by increased glycosaminoglycan release and a unique angiogenic secretory profile from neo-cartilage grafts [Abstract]. International Journal of Experimental Pathology 90(2), pp. A103-A104. (10.1111/j.1365-2613.2009.00644.x)
Roberts, H. C. et al. 2009. Chemically modified glucosamine inhibits the release of proteoglycan in a model system of articular cartilage degradation. International Journal of Experimental Pathology 90(1), pp. A78-A79.
Hurst, S., Rees, S. G., Randerson, P. F., Caterson, B. and Harwood, J. 2009. Contrasting Effects of n-3 and n-6 Fatty Acids on Cyclooxygenase-2 in Model Systems for Arthritis. Lipids 44.(10), pp. 889-896. (10.1007/s11745-009-3347-x)
Roberts, H. C. et al. 2009. Chemically modified glucosamine inhibits the release of proteoglycan in a model system of articular cartilage degradation [Abstract]. International Journal of Experimental Pathology 90(1), pp. A78-A79. (10.1111/j.1365-2613.2008.00612.x)
Rees, S. G. et al. 2009. Immunolocalisation and expression of keratocan in tendon. Osteoarthritis and Cartilage 17(2), pp. 276-279. (10.1016/j.joca.2008.07.007)
Rees, S. G., Dent, C. M. and Caterson, B. 2009. Metabolism of proteoglycans in tendon. Scandinavian Journal of Medicine & Science in Sports 19(4), pp. 470-478. (10.1111/j.1600-0838.2009.00938.x)
Zainal, Z., Longman, A. J., Hurst, S., Duggan, K., Hughes, C. E., Caterson, B. and Harwood, J. 2009. Modification of Palm Oil for Anti-Inflammatory Nutraceutical Properties. Lipids 44(7), pp. 581-592. (10.1007/s11745-009-3304-8)

2008

Davies, L. C., Blain, E. J., Caterson, B. and Duance, V. C. 2008. Chondroitin sulphate impedes the migration of a sub-population of articular cartilage chondrocytes. Osteoarthritis and Cartilage 16(8), pp. 855-864. (10.1016/j.joca.2007.12.005)
Melrose, J. et al. 2008. Fragmentation of decorin, biglycan, lumican and keratocan is elevated in degenerate human meniscus, knee and hip articular cartilages compared with age-matched macroscopically normal and control tissues. Arthritis Research & Therapy 10(4), article number: R79. (10.1186/ar2453)
Khan, I. M., Gilbert, S. J., Caterson, B., Sandell, L. and Archer, C. W. 2008. Oxidative stress induces expression of osteoarthritis markers procollagen IIA and 3B3(-) in adult bovine articular cartilage. Osteoarthritis and Cartilage 16(6), pp. 698-707. (10.1016/j.joca.2007.10.004)
Hayes, A. J., Hughes, C. E. and Caterson, B. 2008. Antibodies and immunohistochemistry in extracellular matrix research. Methods 45(1), pp. 10-21. (10.1016/j.ymeth.2008.01.011)
Cao, J. et al. 2008. Articular cartilage metabolism in patients with Kashin-Beck Disease: an endemic osteoarthropathy in China. Osteoarthritis and Cartilage 16(6), pp. 680-8. (10.1016/j.joca.2007.09.002)
Hayes, A. J., Tudor, D., Nowell, M. A., Caterson, B. and Hughes, C. E. 2008. Chondroitin sulfate sulfation motifs as putative biomarkers for isolation of articular cartilage progenitor cells. Journal of Histochemistry & Cytochemistry 56(2), pp. 125-38. (10.1369/jhc.7A7320.2007)
Akhtar, S., Kerr, B., Hayes, A. J., Hughes, C. E., Meek, K. M. and Caterson, B. 2008. Immunochemical localisation of keratan sulphate proteoglycans in cornea, sclera and limbus using a keratanase generated neoepitope monoclonal antibody. Investigative Ophthalmology and Visual Science 49(6), pp. 2424-2431. (10.1167/iovs.06-1498)
Bondeson, J., Wainwright, S. D., Hughes, C. E. and Caterson, B. 2008. The regulation of ADAMTS4 and ADAMTS5 aggrecanases in osteoarthritis: a review. Clinical and Experimental Rheumatology 26(1), pp. 139-145.
Li, S., Cao, J., Shi, Z., Chen, J., Zhang, Z., Hughes, C. E. and Caterson, B. 2008. Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect. Journal of Zhejiang University-Science B 9(1), pp. 22-33. (10.1631/jzus.B071322)
McGuigan, C. et al. 2008. Phosphate prodrugs derived from N-acetylglucosamine have enhanced chondroprotective activity in explant cultures and represent a new lead in antiosteoarthritis drug discovery. Journal of Medicinal Chemistry 51(18), pp. 5807-5812. (10.1021/jm800594c)
Davies, L. C., Blain, E., Gilbert, S. J., Caterson, B. and Duance, V. 2008. The potential of IGF-1 and TGFβ1 for promoting “adult” articular cartilage repair: An in vitro study. Tissue Engineering Part A 14(7), pp. 1251-1261. (10.1089/ten.tea.2007.0211)
Caterson, B., Hayes, A. J., Tudor, D., Nowell, M. A. and Hughes, C. E. 2008. Chondroitin sulphate motifs as biomarkers for the stem/progenitor cell niche in musculoskeletal tissues. European Cells and Materials 16(Supp 2), pp. 47-47.

2007

Young, R. D., Gealy, E. C., Liles, M., Caterson, B., Ralphs, J. R. and Quantock, A. J. 2007. Keratan sulfate glycosaminoglycan and the association with collagen fibrils in rudimentary lamellae in the developing avian cornea. Investigative Ophthalmology and Visual Science 48(7), pp. 3083-3088. (10.1167/iovs.06-1323)
Rees, S. G., Waggett, A. D., Dent, C. M. and Caterson, B. 2007. Inhibition of aggrecan turnover in short-term explant cultures of bovine tendon. Matrix Biology 26(4), pp. 280-290. (10.1016/j.matbio.2007.01.003)
Bondeson, J. et al. 2007. Adenoviral gene transfer of the endogenous inhibitor IkappaBalpha into human osteoarthritis synovial fibroblasts demonstrates that several matrix metalloproteinases and aggrecanases are nuclear factor-kappaB-dependent. The Journal of Rheumatology 34(3), pp. 523-533.
Young, R. D. et al. 2007. Differential immunogold localisation of sulphated and unsulphated keratan sulphate proteoglycans in normal and macular dystrophy cornea using sulphation motif-specific antibodies. Histochemistry and Cell Biology 127(1), pp. 115-120. (10.1007/s00418-006-0228-8)
Hayes, A. J., Hall, A., Brown, L., Tubo, R. and Caterson, B. 2007. Macromolecular organization and in vitro growth characteristics of scaffold-free neocartilage grafts. Journal of Histochemistry & Cytochemistry 55(8), pp. 853-866. (10.1369/jhc.7A7210.2007)
Gendron, C. et al. 2007. Proteolytic activities of human ADAMTS-5: Comparative studies wth ADAMTS - 4. Journal of Biological Chemistry 282(25), pp. 18294-18306. (10.1074/jbc.M701523200)
Gealy, E. C. et al. 2007. Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis. Histochemistry and Cell Biology 128(6), pp. 551-555. (10.1007/s00418-007-0332-4)
Jones, A. R. et al. 2007. Binding and localization of recombinant lubricin to articular cartilage surfaces. Journal of Orthopaedic Research 25(3), pp. 283-292. (10.1002/jor.20325)
Fang, S. C., Coathup, M. J., Blunn, G. W., Caterson, B., Duance, V. C. and Freeman, M. A. 2007. Fracture healing and its potential as a bioreactor for skeletal tissue engineering. European Cells and Materials 13(Supp 2), pp. 28-29.
Gendron, C. et al. 2007. Proteolytic activities of human ADAMTS-5. comparative studies with human ADAMTS-4 [Erratum]. World Journal of Biological Chemistry 282(38), pp. 28296.

2006

Hayashida, Y. et al. 2006. Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase. PNAS 103(36), pp. 13333-13338. (10.1073/pnas.0605441103)
Grau, S. et al. 2006. The role of human HtrA1 in arthritic disease. The Journal of Biological Chemistry 281(10), pp. 6124-6129. (10.1074/jbc.M500361200)
Young, R. D. et al. 2006. Immunogold localization of keratan sulphate in macular corneal dystrophy using monoclonal antibodies to specific sulphation motifs [Abstract]. International Journal of Experimental Pathology 87(1), pp. A49-A50. (10.1111/j.0959-9673.2006.00458.x)
Gealy, E. C., Buckwell, B., Caterson, B., Quantock, A. J. and Ralphs, J. R. 2006. Association between the cytoskeleton and collagen secretion in the developing cornea [Abstract]. International Journal of Experimental Pathology 87(1), pp. A43-A44. (10.1111/j.0959-9673.2006.00458.x)
Davies, L., Blain, E. J., Caterson, B. and Duance, V. C. 2006. Comparing the responsiveness of chondrocytes isolated from young and mature articular cartilage to IGF-1 and TGFβ1. European Cells and Materials 12(Supp 1), pp. 54-54.

2005

Hall, A. K., Hayes, A. J., Brown, L., Tubo, R. and Caterson, B. 2005. Tissue-engineered cartilage grafts for use in cartilage repair technologies [Abstract]. International Journal of Experimental Pathology 86(3), pp. A9. (10.1111/j.0959-9673.2005.00426.x)
Young, R. D. et al. 2005. Atypical composition and ultrastructure of proteoglycans in the mouse corneal stroma. Investigative Ophthalmology and Visual Science 46(6), pp. 1973-1978. (10.1167/iovs.04-1309)
Johnson, W. E. B., Caterson, B., Eisenstein, S. M. and Roberts, S. 2005. Human intervertebral disc aggrecan inhibits endothelial cell adhesion and cell migration in vitro. Spine 30(10), pp. 1139-1147. (10.1097/01.brs.0000162624.95262.73)
Little, C. B., Flannery, C. R., Hughes, C. E., Goodship, A. and Caterson, B. 2005. Cytokine induced metalloproteinase expression and activity does not correlate with focal susceptibility of articular cartilage to degeneration. Osteoarthritis and Cartilage 13(2) (10.1016/j.joca.2004.10.014)
Innes, J. F., Little, C. B., Hughes, C. E. and Caterson, B. 2005. Products resulting from cleavage of the interglobular domain of aggrecan in samples of synovial fluid collected from dogs with early- and late-stage osteoarthritis. American Journal of Veterinary Research 66(10), pp. 1679-1685. (10.2460/ajvr.2005.66.1679)
Akhtar, S., Davies, J. . R. and Caterson, B. 2005. Ultrastructural immunolocalization of [alpha]-elastin and keratan sulfate proteoglycan in normal and scoliotic lumbar disc. Spine 30(15), pp. 1762-1769. (10.1097/01.brs.0000171912.44625.29)
Rees, S. G., Curtis, C. L., Dent, C. M. and Caterson, B. 2005. Catabolism of aggrecan proteoglycan aggregate components in short-term explant cultures of tendon. Matrix biology 24(3), pp. 219-231. (10.1016/j.matbio.2005.02.002)
Hayes, A. J., Tudor, D., Nowell, M. A., Hughes, C. E. and Caterson, B. 2005. Novel chondroitin sulphation motifs as putative biomarkers of articular cartilage chondroprogenitor cells [Abstract]. European Cells and Materials 10(Supp 2), pp. 72.

2004

Curtis, C., Rees, S., Evans, R., Dent, C., Caterson, B. and Harwood, J. L. 2004. The Effects of n-3 Polyunsaturated Fatty Acids on Cartilage Metabolism in Patients with Osteoarthritis: the Results of a Pilot Clinical Trial. Presented at: 3rd Euro Fed Lipid Congress, Edinburgh, Scotland, 5-8 September 2004.
Johnson, K. A., Francis, D. J., Manley, P. A., Chu, Q. and Caterson, B. 2004. Comparison of the effects of caudal pole hemi-meniscectomy and complete medial meniscectomy in the canine stifle joint. American Journal of Veterinary Research 65(8), pp. 1053-1060.
Kashiwagi, M., Enghild, J. J., Gendron, C., Hughes, C. E., Caterson, B., Itoh, Y. and Nagase, H. 2004. Altered proteolytic activities of ADAMTS-4 expressed by C-terminal processing. Journal of Biological Chemistry 279(11), pp. 10109-10119. (10.1074/jbc.M312123200)
Davies, L. C., Caterson, B. and Duance, V. C. 2004. Novel strategies for enhancing tissue integration in cartilage repair. International Journal of Experimental Pathology 85(1), pp. A21-A22. (10.1111/j.0959-9673.2004.369aa.x)
Janusz, M. J. et al. 2004. Detection of aggrecanase- and MMP-generated catabolic neoepitopes in the rat iodoacetate model of cartilage degeneration. Osteoarthritis and Cartilage 12(9), pp. 720-728. (10.1016/j.joca.2004.06.004)
Curtis, C. L., Harwood, J. L., Dent, C. M. and Caterson, B. 2004. Erratum: Biological basis for the benefit of nutraceutical supplementation in arthritis. Drug Discovery Today 9(7), pp. 336-336. (10.1016/S1359-6446(04)03052-1)
Curtis, C. L., Harwood, J. L., Dent, C. M. and Caterson, B. 2004. Biological basis for the benefit of nutraceutical supplementation in arthritis. Drug Discovery Today 9(4), pp. 165-172. (10.1016/S1359-6446(03)02980-5)
Kashiwagi, M., Enghild, J. J., Gendron, C., Hughes, C., Caterson, B., Itoh, Y. and Nagase, H. 2004. Altered proteolytic activities of ADAMTS-4 expressed by C-terminal processing [Erratum]. World Journal of Biological Chemistry 279(21), pp. 22786.

2003

Harris, C. L., Hughes, C. E., Williams, A. S., Goodfellow, I., Evans, D. J., Caterson, B. and Morgan, B. P. 2003. Generation of anti-complement 'prodrugs': cleavable reagents for specific delivery of complement regulators to disease sites. The Journal of Biological Chemistry 278(38), pp. 36068-36076. (10.1074/jbc.M306351200)
Connon, C. J., Siegler, V., Meek, K. M. A., Hodson, S. A., Caterson, B., Kinoshita, S. and Quantock, A. J. 2003. Proteoglycan alterations and collagen reorganisation in the secondary avian cornea during development. Ophthalmic Research 35(4), pp. 177-184. (10.1159/000071168)
Gendron, C., Kashiwagi, M., Hughes, C. E., Caterson, B. and Nagase, H. 2003. TIMP-3 inhibits aggrecanase-mediated glycosaminoglycan release from cartilage explants stimulated by catabolic factors. FEBS Letters 555(3), pp. 431-436. (10.1016/S0014-5793(03)01295-X)
Hughes, C. E., Little, C. B. and Caterson, B. 2003. Measurement of aggrecanase-generated interglobular domain catabolites in the medium and extracts of cartilage explants using Western blot analysis. In: Winyard, P. G. and Willoughby, D. A. eds. Inflammation Protocols., Vol. 225. Methods in Molecular Biology Humana Press, pp. 89-98., (10.1385/1-59259-374-7:89)
Curtis, C. L., Rees, S. G., Harwood, J. L., Caterson, B. and Dent, C. M. 2003. The effect of n-3 (omega-3) polyunsaturated fatty acids on degenerative joint disease [review]. Agro Food Industry Hi-Tech 14(3), pp. 22-25.

2002

Rees, S. G., Davies, J. R., Tudor, D., Flannery, C. R., Hughes, C. E., Dent, C. M. and Caterson, B. 2002. Immunolocalisation and expression of proteoglycan 4 (cartilage superficial zone proteoglycan) in tendon. Matrix biology 21(7), pp. 593-602. (10.1016/S0945-053X(02)00056-2)
Johnson, W. E. B., Caterson, B., Eisenstein, S. M., Hynds, D. L., Snow, D. M. and Roberts, S. 2002. Human intervertebral disc aggrecan inhibits nerve growth in vitro. Arthritis and Rheumatism 46(10), pp. 2658-2664. (10.1002/art.10585)
Curtis, C. L. et al. 2002. Pathologic indicators of degradation and inflammation in human osteoarthritic cartilage are abrogated by exposure to n-3 fatty acids [RETRACTED]. Arthritis & Rheumatism 46(6), pp. 1544-1553. (10.1002/art.10305)
Johnson, K. A., Hay, C. W., Chu, Q., Roe, S. C. and Caterson, B. 2002. Cartilage-derived biomarkers of osteoarthritis in synovial fluid of dogs with naturally acquired rupture of the cranial cruciate ligament. American Journal of Veterinary Research 63(6), pp. 775-781.
Akhtar, S., Tullo, A. B. 1., Caterson, B., Davies, J. R., Bennett, K. and Meek, K. M. A. 2002. Clinical and morphological features including expression of ÃŸig-h3 and keratan sulphate proteoglycans in Maroteaux-Lamy syndrome type B and in normal cornea. British journal of ophthalmology 86(2), pp. 147-151.
Little, C. B., Hughes, C. E., Jones, S. A., Caterson, B. and Flannery, C. A. 2002. Cyclosporin A inhibition of aggrecanase-mediated proteoglycan catabolism in articular cartilage. Arthritis and Rheumatism - 46(1), pp. 124-129.
Quantock, A. J., Siegler, V., Hughes, C. E. and Caterson, B. 2002. Keratan sulphate levels during development of the embryonic avian cornea. Investigative Ophthalmology and Visual Science 43(E-Abst)
Fox, K. D. and Caterson, B. 2002. Freeing the brain from the perineuronal net. Science 298(5596), pp. 1187-1189. (10.1126/science.1079224)
Akhtar, S., Tullo, A., Caterson, B., Davies, J. R., Bennett, K. and Meek, K. M. A. 2002. Scientific correspondence: clinical and morphological features including expression of βig-h3 and keratan sulphate proteoglycans in Maroteaux-Lamy syndrome type B and in normal cornea. British Journal of Ophthalmology 86(2), pp. 147-151. (10.1136/bjo.86.2.147)
McK. Ciombor, D., Lester, G., Aaron, R. K., Neame, P. and Caterson, B. 2002. Low frequency EMF regulates chondrocyte differentiation and expression of matrix proteins. Journal of Orthopaedic Research 20(1), pp. 40-50. (10.1016/S0736-0266(01)00071-7)
Curtis, C. L. et al. 2002. Effects of n-3 fatty acids on cartilage metabolism [RETRACTED]. Proceedings of the Nutrition Society 61(3), pp. 381-389. (10.1079/PNS2002174)
Little, C. B. et al. 2002. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix biology 21(3), pp. 271-288. (10.1016/S0945-053X(02)00004-5)

2001

Davies, J. R., Williams, A. S., Akhtar, S. and Caterson, B. 2001. Appearance of a proteoglycan layer on the articular surface in murine antigen induced arthritis - Is this an accumulation of superficial zone proteoglycan (SZP)? [Poster Abstract]. International Journal of Experimental Pathology 82(6), pp. A5. (10.1046/j.1365-2613.2001.82_6am.x)
Little, C. B., Hughes, C. E., Otterness, I. G. and Caterson, B. 2001. Species specificity of neoepitope antibodies to aggrecanase and MMP-generated sequences in the interglobular domain of aggrecan [Poster Abstract]. International Journal of Experimental Pathology 82(6), pp. A12. (10.1046/j.1365-2613.2001.82_6am.x)
Roberts, S., Hollander, A., Caterson, B., Menage, J. and Richardson, J. B. 2001. Matrix turnover in human cartilage repair tissue in autologous chondrocyte implantation. Arthritis & Rheumatism 44(11), pp. 2586-2598.
Rees, S. G., Tudor, D., Davies, J. R. and Caterson, B. 2001. Expression and immunolocalization of superficial zone proteoglycan (SZP) in tendon. International Journal of Experimental Pathology 82(6), pp. A16-A17.

2000

Caterson, B., Flannery, C. R., Hughes, C. E. and Little, C. B. 2000. Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biology 19(4), pp. 333-344. (10.1016/S0945-053X(00)00078-0)
Curtis, C. L., Hughes, C. E., Flannery, C. R., Little, C. B., Harwood, J. L. and Caterson, B. 2000. n-3 Fatty Acids Specifically Modulate Catabolic Factors Involved in Articular Cartilage Degradation. Journal of Biological Chemistry 275(2), pp. 721-724. (10.1074/jbc.275.2.721)
Roberts, S., Caterson, B., Menage, J., Evans, E. H., Jaffray, D. C. and Eisenstein, S. M. 2000. Matrix metalloproteinases and aggrecanase. Spine 25(23), pp. 3005-3013. (10.1097/00007632-200012010-00007)
Flannery, C. R., Little, C. B., Hughes, C. E., Curtis, C. L., Caterson, B. and Jones, S. A. 2000. IL-6 and its soluble receptor augment aggrecanase-mediated proteoglycan catabolism in articular cartilage. Matrix biology 19(6), pp. 549-553. (10.1016/S0945-053X(00)00111-6)
Hiscock, D. R. R., Caterson, B. and Flannery, C. R. 2000. Expression of hyaluronan synthases in articular cartilage. Osteoarthritis and Cartilage 8(2), pp. 120-126. (10.1053/joca.1999.0280)
Rees, S. G., Flannery, C. R., Little, C. B., Hughes, C. E., Caterson, B. and Dent, C. M. 2000. Catabolism of aggrecan, decorin and biglycan in tendon. Biochemical Journal 350(1), pp. 181-188. (10.1042/0264-6021:3500181)

Figure1: Monoclonal Antibodies to GAG sulphation Motif Epitopes and Neoepitopes on Aggrecan, Aggrecanase-generated Catabolites and Link Protein

Over the past 27 years Professor Bruce Caterson’s research has focussed on the production, development and use of monoclonal antibody (mAb) technologies for studies of connective tissue proteoglycan metabolism in health and disease. These studies have focussed on matrix proteoglycan metabolism in musculoskeletal tissues with a particular emphasis on studies involving molecular mechanism underlying the pathogenesis of degenerative joint diseases; i.e. osteoarthritis and rheumatoid arthritis. Our lab has now developed and characterised numerous mAbs that recognise both carbohydrate and protein epitopes and neoepitopes that are present on proteoglycans in all connective tissues throughout the body (see Figure 1 and publications, as recent examples). Many of these mAbs are now commercially available to researchers worldwide.

Figure 2:

Immunochemical identification of stem/progenitor cells in the surface/superficial zone of articular cartilage: Immunohistochemical localisation of cell surface and matrix proteoglycans containing native chondroitin sulphate (CS) glycosaminoglycan (GAG) sulphation motifs recognised by monoclonal antibodies 4-C-3(-) and 7-D-4(-). These antibodies identify the pericellular environment surrounding stem/progenitor cells located in the surface/superficial zone of 7-day old bovine articular cartilage. Antibody 4-C-3(-) also recognises CS on proteoglycans surrounding cells (pericytes?) in small blood vessels located in the calcified zone of the immature cartilage.

These reagents and technologies are also being applied to development of new antibody-based biomarker assays to diagnose and monitor the efficacy of therapeutic or surgical interventions in the treatment of degenerative joint diseases and changes in matrix proteoglycan and protein metabolism associated changes in the peri-neuronal net in the pathogenesis of Alzheimer’s disease. Furthermore, these mAb technologies have been applied to tissue engineering & tissue regeneration applications involving the repair of articular cartilage and other musculoskeletal tissues (7, 10). In recent years, these research interests have extended to several collaborative studies investigating proteoglycan metabolism and extracellular matrix organisation in the cornea of the eye during development and with the onset of pathology (2, 4). Very recent work (10) has used several monoclonal antibodies that recognise chondroitin sulphate (CS) glycosaminoglycan sulphation motifs to identify stem/progenitor cells in their “stem cell niches” of musculoskeletal tissues (i.e. cartilage, tendon and intervertebral disc), the eye and the gut of several different animal species (see Figures 2 above & 3 below).

Figure 3:Chondroitin sulphate (CS) glycosaminoglycan (GAG) sulphation motifs and the “stem/progenitor cell niche”:(A) Hyaluronan (HA) bound to CD44 and minimally sulphated CS GAG chains occur on cell surface and matrix proteoglycans and form a protective barrier around stem/progenitor cells in the tissue matrix. These lowly sulphat'ed CS proteoglycans and HA have a low affinity for binding and/or presentation of growth factors, cytokines and chemokines to the dormant stem/progenitor cell. However, many of these growth factors are sequestered on more abundant and highly sulphated CS GAG chains in the territorial and extracellular matrix of the tissue; (B) After being presented with an activating stimulus (e.g. mechanical stress, matrix depletion), the stem/progenitor cell is stimulated to divide and produce two daughter cells. This division physically pushes one of the daughter cells out into the territorial matrix where its cell surface receptors can now interact with growth factors & chemokines already bound to more highly sulphated CS and other GAGs with conformations favourable for receptor binding. (C) These interactions with cell surface receptors stimulate this one daughter cell to proliferate and further differentiate down a chondrocytic lineage. The other daughter cell remains in the “stem/progenitor cell niche” protected from stimulation by growth factors, cytokine and chemokines by the lesser sulphated CS GAGs on proteoglycan and hyaluronan bound to CD44 surrounding this still naive stem/progenitor daughter cell.

Current Funding

Arthritis Research Campaign
BBSRC
EPSRC
Alzheimer’s Research Fund
Industry

Funded Collaborations

Cardiff University

Professor John L. Harwood, Head of School, School of Biosciences: Effects of n-3 fatty acids on brain peri-neuronal net metabolism in the pathogenesis of Alzheimer’s disease.

Mr Colin Dent, FRCS (Orth), Professor of Orthopaedics & Trauma, School of Medicine: Tendon matrix proteoglycan metabolism in health and disease. Biomarkers for musculoskeletal diseases.

and Dr Andrew Quantok, School of Optometry: Corneal matrix organisation and metabolism during development and pathology.

Professor Ruth Duncan, School of Pharmacy and Dr Peter Griffith, School of Chemistry: Nanotechnology drug delivery systems for treatment of arthritic diseases.

Professor Chris McGuigan, School of Pharmacy: The beneficial effects of chemically-modified glucosamine on cartilage metabolism in the pathogenesis of osteoarthritis.

Dr Jan Bondeson, Rheumatology, School of Medicine: Regulation of aggrecanases and matrix metalloproteinase expression in the pathogenesis of degenerative joint diseases.

Dr Jim Ralphs, School of Biosciences: Corneal matrix organisation during development.

National

Dr Sally Roberts & Professor James B. Richardson, RJAH Orthopaedic Hospital, Oswestry: Biomarkers for degenerative joint and disc disease.

Professor Richard Oreffo, Department of Orthopaedics, University of Southhampton: Glycosaminoglycan and polymer scaffolds for tissue engineering and regeneration.

Dr Sarah G. Rees, School of Medicine, Swansea University: Tendon proteoglycan metabolism in health and disease.

International

Dr Virginia Kraus, Department of Rheumatology, Duke University, Durham, NC, USA: Biomarkers of musculoskeletal diseases & cartilage metabolism in the pathogenesis of Kashin-Beck disease.

Professor Junling Cao, Institute of Endemic Diseases, Xi’an Jiaotong Medical University, Xi’an, China: Cartilage metabolism in the pathogenesis of Kashin-Beck Disease.

Dr Chris B. Little, Director, Raymond Purves Institute of Bone and Joint Research, University of Sydney, Australia: Proteoglycan metabolism in cartilage and intervertebral disc disease.

Affiliated Staff

Senior Lecturer

Dr Jim Ralphs

Visiting Clinical Fellow

Mrs Amy Morgan

Scientific Officer

Dr Anthony J. Hayes

Postdoctoral Researchers

Dr Emma Blain

Dr Laurence Glennon-Alty

Dr Helen Roberts

Dr Shane Wainwright

Orthopaedic Surgery Registrar Trainees

Past projects

Proffiliau allanol

ORCID

Unedau Ymchwil

Biofeddygaeth

Yr Athro Bruce Caterson

Trosolwg

Research Overview

Research Division

Bywgraffiad

Cyhoeddiadau

2020

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2012