Dr Sharon Dewitt
Darlithydd
- Ar gael fel goruchwyliwr ôl-raddedig
Bywgraffiad
1999: First degree in Pharmacology, UWCM 2003: PhD from the University of Wales College of Medicine, Cardiff (Phagocytosis by Neutrophils: The Roles of Ca2+) 2004 $acirc; 2010: Wellcome Trust funded Postdoctoral role in the Neutrophil Signalling Group, Cardiff University to investigate the mechanisms of neutrophil function Awarded a Wellcome Trust VIP award to further research in neutrophil phagocytosis. Successful co-applicant of a Wellcome Trust grant, investigating the role of calpain in the control of phagocytosis by neutrophils (with Prof. MB Hallett). Successful co-applicant for a PhD studentship awarded by the MRC and British Lung Foundation as part of the MRC Capacity Building Studentship Competition (with Prof. MB Hallett). 2010 - ARC Cardiff Academic Fellowship (Cardiff University, School of Dentistry) working with Prof Aeschlimann and the ARCBBC team to develop biomarker assays that predict pathology, pain, inflammation and changes in joint function in musculoskeletal diseases Postgraduate Supervision: PhD Co-supervisor: PhD Supervisory Team: Undergraduate Teaching: Year 2 (Bioscience) BI2251 Cellular and Molecular Aspects of Blood. ME3031 Undergraduate Research Project Supervision (co-supervisor). Grants Awarded Medical Research Council Control of neutrophil infiltration into inflamed airways in chronic obstructive pulmonary disease. Capacity Building Studentship (MB Hallett & S Dewitt) 2008-2011. Wellcome Trust Leukocyte trafficking in calpain-1 null mice. Knockout Mouse Funding Committee (MB Hallett, S Dewitt, AS Williams) 2007. Wellcome Trust The role of calpain in the control of phagocytosis by neutrophils (S Dewitt & MB Hallett) 2006-2010. Wellcome Trust Visualisation and manipulation of calpain activity in human neutrophils. Value In People (VIP) Award (S Dewitt) 2004 UWCM Research Initiative Visualisation and manipulation of calpain activity in human neutrophils (S Dewitt) 2004 Personal Awards Wellcome Trust Value In People (VIP) Award, UWCM. April 2004. Research Initiative Award, UWCM. April 2004. 1st Prize Oral Presentation at the UWCM 16th Annual Postgraduate Research Day, 2001. Presentations: American Society of Cell Biology 46th Annual Meeting, San Diego, 2006. &lcirc; -Calpain Activation and Re-distribution in Myeloid Cells Induced by Elevated Cytosolic Free Ca2+ and Phagocytosis Infection, Immunity and Inflammation IRG Annual Meeting, Millennium Stadium Cardiff, 2006. Regulation of neutrophil infiltration into rheumatoid joints by mu-calpain ASCB San Francisco 45th Annual Meeting, 2005. Localised PI(3,4,5)P3 at the Phagocytic Cup in HL60 Neutrophils. European Society Clinical Investigation Meeting, Utrecht 2004. Calpain activity during beta-2 integrin-mediated phagocytosis. Biochemical Society, Calcium Oscillations and the 5th UK Calcium Signalling Conference, Liverpool 2003. Multi-component Ca2+ signalling accompanies phagocytosis by neutrophils. Royal Microscopical Society Annual Cell Biology and Histochemistry Meeting, London 2003. Complex Ca2+ signalling in neutrophils co-ordinates their phagocytic and oxidative activity. UWCM 16th Annual Postgraduate Research Day, 2001. Cytosolic free Ca2+ changes and calpain activity are required for integrin-accelerated phagocytosis by neutrophils. UWCM 15th Annual Postgraduate Research Day, 2000. Dissecting neutrophil phagocytosis: The role of Ca2+ signalling.Career Profile
Teaching Profile
Awards / Prizes
Cyhoeddiadau
2022
- Brown, S. V., Dewitt, S., Clayton, A. and Waddington, R. J. 2022. Identifying the efficacy of extracellular vesicles in osteogenic differentiation: an EV-Lution in regenerative medicine. Frontiers in Dental Medicine 3, article number: 849724. (10.3389/fdmed.2022.849724)
2020
- Al-Jumaa, M., Hallett, M. B. and Dewitt, S. 2020. Cell surface topography controls phagocytosis and cell spreading: the membrane reservoir in neutrophils. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 12, article number: 118832. (10.1016/j.bbamcr.2020.118832)
- Dewitt, S. and Hallett, M. B. 2020. Calpain activation by Ca2+ and its role in phagocytosis. In: Hallett, M. B. ed. Molecular and Cellular Biology of Phagocytosis., Vol. 1246. Advances in Experimental Medicine and Biology Switzerland: Springer, pp. 129-151., (10.1007/978-3-030-40406-2_8)
- Roberts, R. E., Dewitt, S. and Hallett, M. B. 2020. Membrane tension and the role of ezrin during phagocytosis. In: Hallett, M. B. ed. Molecular and Cellular Biology of Phagocytosis., Vol. 1246. Advances in Experimental Medicine and Biology Switzerland: Springer, pp. 83-102., (10.1007/978-3-030-40406-2_6)
2019
- Hallett, M. B., Roberts, R. E. and Dewitt, S. 2019. Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils. In: Neutrophil., Vol. 2087. Methods in Molecular Biology New York: Humana Press, pp. 191-205., (10.1007/978-1-0716-0154-9_14)
2017
- Jumaa, M. A. A., Dewitt, S. and Hallett, M. B. 2017. Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading. Scientific Reports 7, pp. -., article number: 9790. (10.1038/s41598-017-09761-6)
2015
- Adamczyk, M., Griffiths, R., Dewitt, S., Knauper, V. and Aeschlimann, D. 2015. P2X7 receptor activation regulates rapid unconventional export of transglutaminase-2. Journal of Cell Science 128(24), pp. 4615-4628. (10.1242/jcs.175968)
- Wanger, T. M., Dewitt, S., Collins, A., Maitland, N. J., Poghosyan, Z. and Knauper, V. 2015. Differential regulation of TROP2 release by PKC isoforms through vesicles and ADAM17. Cellular Signalling 27(7), pp. 1325-1335. (10.1016/j.cellsig.2015.03.017)
2014
- Lewis, K. J., Masterman, B., Laffafian, I., Dewitt, S., Campbell, J. S. and Hallett, M. B. 2014. Minimal impact electro-injection of cells undergoing dynamic shape change reveals calpain activation. BBA - Molecular Cell Research 1843(6), pp. 1182-1187. (10.1016/j.bbamcr.2014.02.020)
- Hallett, M., Al-Jumaa, M. and Dewitt, S. 2014. Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils. In: Quinn, M. T. and DeLeo, F. R. eds. Neutrophil Methods and Protocols. Methods in Molecular Biology Vol. 1124. Humana Press, pp. 107-120., (10.1007/978-1-62703-845-4_8)
2013
- Dewitt, S., Francis, R. J. and Hallett, M. B. 2013. Ca2+ and calpain control membrane expansion during rapid cell spreading of neutrophils. Journal of Cell Science 126(20), pp. 4627-4635. (10.1242/jcs.124917)
2011
- McDonald, J. U. et al. 2011. In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils. The FASEB Journal 25(6), pp. 1972-1982. (10.1096/fj.10-178517)
- Dewitt, S. and Hallett, M. B. 2011. Optical complexities of living cytoplasm - implications for live cell imaging and photo-micromanipulation techniques. Journal of Microscopy 241(3), pp. 221-224. (10.1111/j.1365-2818.2010.03451.x)
- Elumalai, G. L., Dewitt, S. and Hallett, M. B. 2011. Ezrin and talin relocates from the plasma membrane to cytosol during neutrophil extravasation [Abstract]. European Journal of Clinical Investigation 41(s1), pp. 47. (10.1111/j.1365-2362.2011.02508.x)
- Lewis, K., Dewitt, S. and Hallett, M. B. 2011. The role of calpain translocation in neutrophil spreading [Abstract]. European Journal of Clinical Investigation 41(s1), pp. 47. (10.1111/j.1365-2362.2011.02508.x)
- Ishak, R., Dewitt, S. and Hallett, M. B. 2011. Calpain-1: investigating its role in murine neutrophils [Abstract]. European Journal of Clinical Investigation 41(s1), pp. 24. (10.1111/j.1365-2362.2011.02508.x)
- Hallett, M. B. and Dewitt, S. 2011. A trick of the light: the optical properties of living cytoplasm which can mislead. Integrative Biology 3(3), pp. 180-184. (10.1039/c0ib00039f)
2010
- Brasen, J. C., Dewitt, S. and Hallett, M. B. 2010. A reporter of UV intensity delivered to the cytosol during photolytic uncaging. Biophysical Journal 98(7), pp. L25-L27. (10.1016/j.bpj.2009.12.4271)
2009
- Dewitt, S., Darley, R. L. and Hallett, M. B. 2009. Translocation or just location? Pseudopodia affect fluorescent signals. Journal of Cell Biology 184(2), pp. 197-203. (10.1083/jcb.200806047)
2008
- Hallett, M. . B., Von Ruhland, C. J. and Dewitt, S. 2008. Chemotaxis and the cell surface-area problem [Letter]. Nature Reviews Molecular Cell Biology 9(8), pp. 662. (10.1038/nrm2419-c1)
2007
- Dewitt, S. and Hallett, M. B. 2007. Leukocyte membrane 'expansion': a central mechanism for leukocyte extravasation. Journal of leukocyte biology 81(5), pp. 1160-1164. (10.1189/jlb.1106710)
- Hillson, E. J., Dewitt, S. and Hallett, M. B. 2007. Optical methods for the measurement and manipulation of cytosolic free calcium in neutrophils. In: Quinn, M. T., DeLeo, F. R. and Bokoch, G. M. eds. Neutrophil Methods and Protocols. Methods in Molecular Biology Vol. 412. Totowa, NJ: Humana Press, pp. 125-137., (10.1007/978-1-59745-467-4_10)
2006
- Hallett, M. B., Dewitt, S. and Tian, W. 2006. Localised PtdIns(3,4,5)P3 or PtdIns(3,4)P2 at the phagocytic cup is required for both phagosome closure and Ca2+ signalling in HL60 neutrophils. Journal of Cell Science 119(3), pp. 443-51. (10.1242/jcs.02756)
2004
- Ayub, K., Laffaffian, I., Dewitt, S. and Hallett, M. B. 2004. Ca2+ influx shutdown in neutrophils induced by Fas (CD95) cross-linking. Immunology 112(3), pp. 454-460. (10.1111/j.1365-2567.2004.01899.x)
- Clayton, A., Turkes, A., Dewitt, S., Steadman, R., Mason, M. D. and Hallett, M. B. 2004. Adhesion and signaling by B cell-derived exosomes: the role of integrins. The FASEB Journal 18, pp. 977-979. (10.1096/fj.03-1094fje)
2003
- Tian, W., Laffafian, I., Dewitt, S. and Hallett, M. B. 2003. Exclusion of exogenous phosphatidylinositol-3,4,5-trisphosphate from neutrophil-polarizing pseudopodia: stabilization of the uropod and cell polarity. EMBO Reports 4(10), pp. 982-988. (10.1038/sj.embor.embor947)
- Hallett, M. B., Laffafian, I. and Dewitt, S. 2003. Phagosomal oxidative activity during beta2 integrin (CR3)-mediated phagocytosis by neutrophils is triggered by a non-restricted Ca2+ signal: Ca2+ controls time not space. Journal of Cell Science 116(14), pp. 2857-65. (10.1242/jcs.00499)
- Dewitt, S., Laffafian, I., Morris, M. R. and Hallett, M. B. 2003. Cytosolic Ca2+ measurement and imaging in inflammatory cells. In: Winyard, P. G. and Willoughby, D. A. eds. Inflammation Protocols. Methods in Molecular Biology Vol. 255. Totowa, NJ: Humana Press, pp. 47-63., (10.1385/1-59259-374-7:47)
- Hallett, M. B., Cole, C. and Dewitt, S. 2003. Detection and visualization of oxidase activity in phagocytes. In: Winyard, P. G. and Willoughby, D. A. eds. Inflammation Protocols., Vol. 225. Methods in Molecular Biology Totowa, NJ: Humana Press, pp. 61-67., (10.1385/1-59259-374-7:61)
- Morris, M. R., Dewitt, S., Laffafian, I. and Hallett, M. B. 2003. Phagocytosis by inflammatory phagocytes; experimental strategies for stimulation and quantification. In: Winyard, P. G. and Willoughby, D. A. eds. Inflammation Protocols. Methods in Molecular Biology Vol. 225. Totowa, NJ: Humana Press, pp. 35-46., (10.1385/1-59259-374-7:35)
2002
- Hallett, M. B. and Dewitt, S. 2002. Cytosolic free Ca(2+) changes and calpain activation are required for beta integrin-accelerated phagocytosis by human neutrophils. The Journal of Cell Biology 159(1), pp. 181-9. (10.1083/jcb.200206089)
