Dr Pierre Rizkallah
Rcuk Fellow
- rizkallahp@cardiff.ac.uk
- +44 (0)29 2074 2248
- Adeilad Ymchwil Cardiofasgwlaidd Syr Geraint Evans, Ysbyty Athrofaol Cymru, Parc y Mynydd Bychan, Caerdydd, CF14 4XN
- Sylwebydd y cyfryngau
- Ar gael fel goruchwyliwr ôl-raddedig
Trosolwg
I am a Chemistry graduate, at BSc and PhD level. I use Physics applications to study biological macromolecules, a field known as Structural Biology. I have extensive experience with X-ray crystallography and 3D structure determination using synchrotron radiation. I have used the allied techniques of Small Angle X-ray Scattering (SAXS) and Circular Dichroism (CD) to shed light on biological mechanisms.
I have served on committees and participated in programs at national and international levels. I have conducted many outreach and media exercises during the course of my career, communicating the immediacy relevance and importance of Science in everyday life.
I had a brief period of experience in the cosmetics industry, leading a production line team.
Cyhoeddiadau
2022
- Best, H. L., Williamson, L. J., Lipka-Lloyd, M., Waller-Evans, H., Lloyd-Evans, E., Rizkallah, P. J. and Berry, C. 2022. The crystal structure of Bacillus thuringiensis Tpp80Aa1 and its interaction with galactose-containing glycolipids. Toxins 14(12), article number: 863. (10.3390/toxins14120863)
- Dolton, G. et al. 2022. Emergence of immune escape at dominant SARS-CoV-2 killer T cell epitope. Cell 185(16), pp. 2936-2951. (10.1016/j.cell.2022.07.002)
- Bates, E. A. et al. 2022. Development of a low seroprevalence, αvβ6 integrin selective virotherapy based on human adenovirus type 10. Molecular Therapy - Oncolytics 25, pp. 43-56. (10.1016/j.omto.2022.03.007)
- Noby, N. et al. 2022. Structure-guided engineering of a family IV cold-adapted esterase expands its substrate range. International Journal of Molecular Sciences 23(9), article number: 4703. (10.3390/ijms23094703)
2021
- Noby, N. et al. 2021. Structure and in silico simulations of a cold-active esterase reveals its prime cold-adaptation mechanism. Open Biology 11(12), article number: 210182. (10.1098/rsob.210182)
- Baker, A. T. et al. 2021. ChAdOx1 interacts with CAR and PF4 with implications for thrombosis with thrombocytopenia syndrome. Science Advances 7(49), article number: eabl8213. (10.1126/sciadv.abl8213)
- Griffiths, J. S. et al. 2021. A human Dectin-2 deficiency associated with invasive aspergillosis. Journal of Infectious Diseases 224(7), pp. 1219-1224. (10.1093/infdis/jiab145)
- Man, S. et al. 2021. Synthetic peptides with inadvertent chemical modifications can activate potentially autoreactive T cells. Journal of Immunology 207(4), pp. 1009-1017. (10.4049/jimmunol.2000756)
- Worthy, H. L. et al. 2021. The crystal sructure of Bacillus cereus HblL1. Toxins 13(4), article number: 253. (10.3390/toxins13040253)
- Baker, A. T. et al. 2021. The fiber knob protein of human adenovirus type 49 mediates highly efficient and promiscuous infection of cancer cell lines using a novel cell entry mechanism. Journal of Virology 95(4), article number: e01849-20. (10.1128/JVI.01849-20)
- Pope, J. R. et al. 2021. Association of fluorescent protein pairs and it's significant impact on fluorescence and energy transfer. Advanced Science 8(1), article number: 2003167. (10.1002/advs.202003167)
2020
- Mowbray, N. G. et al. 2020. Safe management of surgical smoke in the age of COVID-19. British Journal of Surgery 107(11), pp. 1406-1413. (10.1002/bjs.11679)
- Bovay, A. et al. 2020. Identification of a superagonist variant of the immunodominant Yellow fever virus epitope NS4b214-222 by combinatorial peptide library screening. Molecular Immunology 125, pp. 43-50. (10.1016/j.molimm.2020.06.025)
- Greenshields-Watson, A. et al. 2020. CD4 + T cells recognize conserved influenza A epitopes through shared patterns of V-Gene usage and complementary biochemical features. Cell Reports 32(2), article number: 107885. (10.1016/j.celrep.2020.107885)
- Holland, C. J. et al. 2020. Specificity of bispecific T cell receptors and antibodies targeting peptide-HLA. Journal of Clinical Investigation 130(5), pp. 2673–2688. (10.1172/JCI130562)
- Cresser-Brown, J., Rizkallah, P., Jin, Y., Roth, C., Miller, D. J. and Allemann, R. K. 2020. An unexpected co-crystal structure of the calpain PEF(S) domain with Hfq reveals a potential chaperone function of Hfq. Acta Crystallographica Section F: Structural Biology Communications 76(2), pp. 81-85. (10.1107/S2053230X20001181)
2019
- MacLachlan, B. J. et al. 2019. Human leukocyte antigen (HLA) class II peptide flanking residues tune the immunogenicity of a human tumor-derived epitope. Journal of Biological Chemistry 294(52), pp. 20246-20258. (10.1074/jbc.RA119.009437)
- Worthy, H. L. et al. 2019. Positive functional synergy of structurally integrated artificial protein dimers assembled by Click chemistry. Communications Chemistry 2, article number: 83. (10.1038/s42004-019-0185-5)
- Madura, F. et al. 2019. TCR-induced alteration of primary MHC peptide anchor residue. European Journal of Immunology 49(7), pp. 1052-1066. (10.1002/eji.201948085)
- Kalvaitis, M. E., Johnson, L. A., Mart, R. J., Rizkallah, P. and Allemann, R. K. 2019. A noncanonical chromophore reveals structural rearrangements of the light-oxygen-voltage domain upon photoactivation. Biochemistry 58(22), pp. 2608-2616. (10.1021/acs.biochem.9b00255)
- Scott, A. F., Cresser-Brown, J., Williams, T. L., Rizkallah, P. J., Jin, Y., Luk, L. Y. and Allemann, R. K. 2019. Crystal structure and biophysical analysis of furfural detoxifying aldehyde reductase from clostridium beijerinkii. Applied and Environmental Microbiology (10.1128/AEM.00978-19)
- Galloway, S. A. E. et al. 2019. Peptide super-agonist enhances T-cell responses to melanoma. Frontiers in Immunology 10, article number: 319. (10.3389/fimmu.2019.00319)
- Baker, A. T. et al. 2019. Diversity within the adenovirus fiber knob hypervariable loops influences primary receptor interactions. Nature Communications 10, article number: 741. (10.1038/s41467-019-08599-y)
2018
- Uusi-Kerttula, H. et al. 2018. Ad5NULL-A20 - a tropism-modified, αvβ6 integrin-selective oncolytic adenovirus for epithelial ovarian cancer therapies. Clinical Cancer Research 24(17), pp. 4215-4224. (10.1158/1078-0432.CCR-18-1089)
- Tungatt, K. et al. 2018. Induction of influenza-specific local CD8 T-cells in the respiratory tract after aerosol delivery of vaccine antigen or virus in the Babraham inbred pig. PLoS Pathogens 14(5), article number: e1007017. (10.1371/journal.ppat.1007017)
- Holland, C. J. et al. 2018. In silico and structural analyses demonstrate that intrinsic protein motions guide T cell receptor complementarity determining region loop flexibility. Frontiers in Immunology 9, article number: 674. (10.3389/fimmu.2018.00674)
- Miles, J. J. et al. 2018. Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry. Journal of Clinical Investigation 128(4), pp. 1569-1580. (10.1172/JCI91512)
- Bovay, A. et al. 2018. T cell receptor alpha variable 12-2 bias in the immunodominant response to Yellow fever virus. European Journal of Immunology 48(2), pp. 258-272. (10.1002/eji.201747082)
2017
- Cole, D. K. et al. 2017. Dual molecular mechanisms govern escape at immunodominant HLA A2-restricted HIV epitope. Frontiers in Immunology 8, article number: 1503. (10.3389/fimmu.2017.01503)
- MacLachlan, B. J. et al. 2017. Using X-ray crystallography, biophysics, and functional assays to determine the mechanisms governing T-cell receptor recognition of cancer antigens. Journal of Visualized Experiments(120), article number: e54991. (10.3791/54991)
- Cole, D. K. et al. 2017. Structural mechanism underpinning cross-reactivity of a CD8+ T-cell clone that recognises a peptide derived from human telomerase reverse transcriptase. Journal of Biological Chemistry 292(3), pp. 802-813. (10.1074/jbc.M116.741603)
2016
- Nomikos, M. et al. 2016. Mutations in PLCd1 associated with hereditary leukonychia display divergent PIP2 hydrolytic function. FEBS Journal 283(24), pp. 4502-4514. (10.1111/febs.13939)
- Hartley, A. M., Worthy, H. L., Reddington, S. C., Rizkallah, P. J. and Jones, D. D. 2016. Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle. Chemical Science 7(10), pp. 6484-6491. (10.1039/C6SC00944A)
- Cole, D. K. et al. 2016. Hotspot autoimmune T cell receptor binding underlies pathogen and insulin peptide cross-reactivity. Journal of Clinical Investigation 126(6), pp. 2191-2204. (10.1172/JCI85679)
- Bianchi, V. et al. 2016. A molecular switch abrogates glycoprotein 100 (gp100) T-cell Receptor (TCR) targeting of a human melanoma antigen. Journal of Biological Chemistry 291(17), pp. 8951-8959. (10.1074/jbc.M115.707414)
- Raman, M. C. C. et al. 2016. Direct molecular mimicry enables off-target cardiovascular toxicity by an enhanced affinity TCR designed for cancer immunotherapy. Scientific Reports 6, article number: 18851. (10.1038/srep18851)
2015
- Adams, S., Robinson, E., Miller, D. J., Rizkallah, P., Hallett, M. B. and Allemann, R. K. 2015. Conformationally restricted calpain inhibitors. Chemical Science 6(12), pp. 6865-6871. (10.1039/c5sc01158b)
- Motozono, C. et al. 2015. Distortion of the major histocompatibility complex class I binding groove to accommodate an insulin-derived 10-Mer peptide. Journal of Biological Chemistry 290(31), pp. 18924-18933. (10.1074/jbc.M114.622522)
- Reid, R., Redman, J. E., Rizkallah, P., Fegan, C. D., Pepper, C. J. and Man, S. T. 2015. CD8+ T-cell recognition of a synthetic epitope formed by t-butyl modification. Immunology 144(3), pp. 495-505. (10.1111/imm.12398)
- Madura, F. et al. 2015. Structural basis for ineffective T-cell responses to MHC anchor residue-improved 'heteroclitic' peptides. European Journal of Immunology 45(2), pp. 584-591. (10.1002/eji.201445114)
- Kløverpris, H. N. et al. 2015. A molecular switch in immunodominant HIV-1-specific CD8 T-cell epitopes shapes differential HLA-restricted escape. Retrovirology 12(1), article number: 20. (10.1186/s12977-015-0149-5)
- Reddington, S. C., Driezis, S., Hartley, A. M., Watson, P. D., Rizkallah, P. J. and Jones, D. D. 2015. Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein. RSC Advances 5(95), pp. 77734-77738. (10.1039/C5RA13552D)
2014
- Adams, S. E., Rizkallah, P., Miller, D. J., Robinson, E. J., Hallett, M. B. and Allemann, R. K. 2014. The structural basis of differential inhibition of human calpain by indole and phenyl α--mercaptoacrylic acids. Journal of Structural Biology 187(3), pp. 236-241. (10.1016/j.jsb.2014.07.004)
- Nomikos, M. et al. 2014. Altered RyR2 regulation by the calmodulin F90L mutation associated with idiopathic ventricular fibrillation and early sudden cardiac death. Febs Letters 588(17), pp. 2898-2902. (10.1016/j.febslet.2014.07.007)
- Arpino, J. J., Reddington, S. C., Halliwell, L., Rizkallah, P. and Jones, D. D. 2014. Random single amino acid deletion sampling unveils structural tolerance and the benefits of helical registry shift on GFP folding and structure. Structure 22(6), pp. 889-898. (10.1016/j.str.2014.03.014)
- Motozono, C. et al. 2014. Molecular basis of a dominant T cell response to an HIV reverse transcriptase 8-mer epitope presented by the protective allele HLA-B*51:01. The Journal of Immunology 192(7), pp. 3428-3434. (10.4049/jimmunol.1302667)
- Arpino, J. A. J., Rizkallah, P. and Jones, D. D. 2014. Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein. Acta Crystallographica Section D Biological Crystallography 70(8), pp. 2152-2162. (10.1107/S139900471401267X)
2013
- Cole, D. et al. 2013. Increased peptide contacts govern high affinity binding of a modified TCR whilst maintaining a native pMHC docking mode. Frontiers in Immunology 4, article number: 168. (10.3389/fimmu.2013.00168)
- Reddington, S. C., Rizkallah, P., Watson, P. D., Pearson, R., Tippmann, E. M. and Jones, D. D. 2013. Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence. Angewandte Chemie - International Edition 52(23), pp. 5974-5977. (10.1002/anie.201301490)
- Madura, F. et al. 2013. T-cell receptor specificity maintained by altered thermodynamics. Journal of Biological Chemistry 288(26), pp. 18766-18775. (10.1074/jbc.M113.464560)
- Eukeruche-Makinde, J. et al. 2013. Peptide length determines the outcome of TCR/peptide-MHCI engagement. Blood -New York- 121(7), pp. 1112-1123. (10.1182/blood-2012-06-437202)
- Ekeruche, J. et al. 2013. Peptide length determines the outcome of TCR/peptide-MHCI engagement. Blood 121(7), pp. 1112-1123. (10.1182/blood-2012-06-437202)
- Cole, D. et al. 2013. T-cell Receptor (TCR)-Peptide Specificity Overrides Affinity-enhancing TCR-Major Histocompatibility Complex Interactions. Journal of Biological Chemistry 289(2), pp. 628-638. (10.1074/jbc.M113.522110)
- Reddington, S. C., Watson, P. D., Rizkallah, P., Tippmann, E. M. and Jones, D. D. 2013. Genetically encoding phenyl azide chemistry: new uses and ideas for classical biochemistry. Biochemical Society Transactions 41(5), pp. 1177-1182. (10.1042/BST20130094)
2012
- Ekeruche, J. et al. 2012. T-cell receptor-optimized peptide skewing of the T-cell repertoire can enhance antigen targeting. Journal of Biological Chemistry 287(44), pp. 37269-37281. (10.1074/jbc.M112.386409)
- Ekeruche-Makinde, J. et al. 2012. T-cell receptor-optimized peptide skewing of the T-cell repertoire can enhance antigen targeting. Journal of Biological Chemistry 287(44), pp. 37269-37281. (10.1074/jbc.M112.386409)
- Holland, C. J. et al. 2012. Minimal conformational plasticity enables TCR cross-reactivity to different MHC class II heterodimers. Scientific Reports 2, article number: 629. (10.1038/srep00629)
- Bulek, A. M. et al. 2012. TCR/pMHC optimized protein crystallization screen. Journal of Immunological Methods 382(1-2), pp. 203-210. (10.1016/j.jim.2012.06.007)
- Yang, S. et al. 2012. A partially interpenetrated metal-organic framework for selective hysteretic sorption of carbon dioxide. Nature Materials 11(8), pp. 710-716. (10.1038/nmat3343)
- Bulek, A. et al. 2012. Structural basis for the killing of human beta cells by CD8+ T cells in type 1 diabetes. Nature Immunology 13(3), pp. 283-289. (10.1038/ni.2206)
- Bulek, A. M. et al. 2012. Structural basis for the killing of human beta cells by CD8+ T cells in type 1 diabetes. Nature Immunology 13(3), pp. 283-289. (10.1038/ni.2206)
- Arpino, J., Rizkallah, P. and Jones, D. D. 2012. Crystal structure of enhanced green fluorescent protein to 1.35 Å resolution reveals alternative conformations for Glu222. PLoS ONE 7(10), article number: e47132. (10.1371/journal.pone.0047132)
- Tadesse, H., Blake, A. J., Champness, N. R., Warren, J. E., Rizkallah, P. and Licence, P. 2012. Supramolecular architectures of symmetrical dicationic ionic liquid based systems. Crystengcomm 14(15), pp. 4886-4893. (10.1039/c2ce25106j)
2011
- Lane, S. W., Dennis, C. A., Lane, C. L., Trinh, C. H., Rizkallah, P., Stockley, P. G. and Phillips, S. E. V. 2011. Construction and crystal structure of recombinant STNV capsids. Journal of Molecular Biology 413(1), pp. 41-50. (10.1016/j.jmb.2011.07.062)
2010
- Miles, J. J. et al. 2010. Genetic and structural basis for selection of a ubiquitous T cell receptor deployed in Epstein-Barr virus infection. PLoS Pathogens 6(11), article number: e1001198. (10.1371/journal.ppat.1001198)
- Ronson, T. K., Carruthers, C., Fisher, J., Brotin, T., Harding, L. P., Rizkallah, P. and Hardie, M. J. 2010. Tripodal 4-pyridyl-derived host ligands and their metallo-supramolecular chemistry: stella octangula and bowl-shaped assemblies. Inorganic Chemistry -American Chemical Society- 49(2), pp. 675-685. (10.1021/ic901972h)
2009
- Ronson, T. K., Fisher, J., Harding, L. P., Rizkallah, P., Warren, J. E. and Hardie, M. J. 2009. Stellated polyhedral assembly of a topologically complicated Pd4L4 'Solomon cube'. Nature Chemistry 1(3), pp. 212-216. (10.1038/nchem.213)
- Cole, D. et al. 2009. Germ line-governed recognition of a cancer epitope by an immunodominant human T-cell receptor. Journal of Biological Chemistry 284(40), article number: 27281-27289. (10.1074/jbc.M109.022509)
2008
- Govada, L. et al. 2008. Crystal structure of the C1 domain of cardiac myosin binding protein-C: Implications for hypertrophic cardiomyopathy. Journal of Molecular Biology 378(2), pp. 387-397. (10.1016/j.jmb.2008.02.044)
- Westcott, A., Fisher, J., Harding, L. P., Rizkallah, P. and Hardie, M. J. 2008. Self-assembly of a 3-D triply interlocked chiral [2] catenane. Journal of the American Chemical Society 130(10), pp. 2950-2951. (10.1021/ja8002149)
2007
- Cole, D. et al. 2007. Computational design and crystal structure of an enhanced affinity mutant human CD8 αα coreceptor. Proteins: Structure, Function and Bioinformatics 67(1), pp. 65-74. (10.1002/prot.21176)
2006
- Cole, D. et al. 2006. Crystal structure of HLA-A*2402 complexed with a telomerase peptide. European Journal of Immunology 36(1), pp. 170-179. (10.1002/eji.200535424)
2005
- Cole, D. et al. 2005. Crystallization and preliminary X-ray structural studies of a high-affinity CD8αα co-receptor to pMHC. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 61(3), pp. 285-287. (10.1107/S1744309105002988)