Yr Athro Maurice Hallett

Professor

: hallettmb@cardiff.ac.uk
: +44 292074 2748
: UHW Main Building

: Sylwebydd y cyfryngau

My research is currently within the Division of Infection and Immunity and is focussed on neutrophil behaviour and signalling at the individual cell level..

I established the Neutrophil Siganlling Group within Cardiff Univesity Medical School with the aim of using novel technques to undestand and influence the activity of these cells which are impratnt in inflammatory disease.

For a number of years, the Neutrophil Signalling lab has studied signalling in living human neutrophils. In this way, the link between the chemical change within the neutrophil and its responses have been observed and the effect of experimental interventions established. This has necessitated the invention of some novel micro-manipulation and imaging techniques. The individual cell approach, however, has revealed an insight into how neutrophils are able to carry out their work-programme in a co-ordinated and effective manner. It also points towards therapeutic targets which may be beneficially manipulated in inflammatory disease.

We have established that an important Ca2+ “read-out” is the Ca2+ activated protease, mu-calpain activation. Since the action of the protease may be long-lived (or irreversible), this would provide a molecular memory of previous Ca2+ signalling within the cell. This has become an important focus for progress towards understanding the link between Ca2+ signals and physiological or pathological responses by the neutrophils.

Professor of Experimental Cell Biology: currently Emeritus Professor (active).

Education

First degree BSc (Pharmacology) University College London (UCL)

PhD Department of Pharmacology, University College London (Ca2+ in mast cell secretion)

FRMS

Anrhydeddau a Dyfarniadau

The group has received funding from MRC, Wellcome Trust, BBSRC, Arthritis and Research Campaign (ARC), BHF, National Research Network, Cystic Fibrosis Trust and others.

Aelodaethau proffesiynol

FRMS Memberships/External Activities

Biochemical Society

British Soc Cell Biology

Safleoedd academaidd blaenorol

Professor Emeritus

New Blood Lecturer, Senior Lecturer, Reader and Professor at Cardiff University

Director of Postgraduate Research (MEDIC)

Ymrwymiadau siarad cyhoeddus

Invited Talks include:-

William Harvey Research Institute,

University of London

Pasteur Institute, Athens

Birmingham Univerity (Medical School)

Cambridge University (Genetics)

Oxford University (Pharmacology)

Babraham Institute

Edinburgh University (MRC Inflammation)

Queens University Belfast

CRUK

Pwyllgorau ac adolygu

I have served on many committees within the University.

Externally I have served on the

Royal Microscopical Society Comitee (Cell Biology) mermbarer and then Hon Sec

Biochemical Soc (Cell Biology Theme comittee)

2019

Hallett, M. B.et al. 2019. Microinjection and micropipette-controlled phagocytosis methods for neutrophils. In: Neutrophil., Vol. 2087. Methods in Molecular Biology New York: Humana Press, pp. 117-125., (10.1007/978-1-0716-0154-9_9)
Hallett, M. B., Roberts, R. E. and Dewitt, S. 2019. Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils. In: Neutrophil., Vol. 2087. Methods in Molecular Biology New York: Humana Press, pp. 191-205., (10.1007/978-1-0716-0154-9_14)
Jennings, S. and Hallett, M. B. 2019. Single cell measurement of calpain activity in neutrophils reveals link to cytosolic Ca2+ elevation and individual phagocytotic events. Biochemical and Biophysical Research Communications 515(1), pp. 163-168. (10.1016/j.bbrc.2019.05.125)
Roberts, R. and Hallett, M. 2019. Neutrophil cell shape change: mechanism and signalling during cell spreading and phagocytosis. International Journal of Molecular Sciences 20(6), article number: 1383. (10.3390/ijms20061383)

2014

Adams, S. E.et al. 2014. The structural basis of differential inhibition of human calpain by indole and phenyl α--mercaptoacrylic acids. Journal of Structural Biology 187(3), pp. 236-241. (10.1016/j.jsb.2014.07.004)
Lewis, K. J.et al. 2014. Minimal impact electro-injection of cells undergoing dynamic shape change reveals calpain activation. BBA - Molecular Cell Research 1843(6), pp. 1182-1187. (10.1016/j.bbamcr.2014.02.020)
Hallett, M., Al-Jumaa, M. and Dewitt, S. 2014. Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils. In: Quinn, M. T. and DeLeo, F. R. eds. Neutrophil Methods and Protocols. Methods in Molecular Biology Vol. 1124. Humana Press, pp. 107-120., (10.1007/978-1-62703-845-4_8)
Laffafian, I.et al. 2014. Microinjection methods for neutrophils. In: Quinn, M. T. and DeLeo, F. R. eds. Neutrophil Methods and Protocols. Methods in Molecular Biology Vol. 1124. Humana Press, pp. 181-187., (10.1007/978-1-62703-845-4_11)

2013

Miller, D. J.et al. 2013. Calpain-1 inhibitors for selective treatment of rheumatoid arthritis: what is the future?. Future Medicinal Chemistry 5(17), pp. 2057-2074. (10.4155/fmc.13.172)
Dewitt, S., Francis, R. J. and Hallett, M. B. 2013. Ca2+ and calpain control membrane expansion during rapid cell spreading of neutrophils. Journal of Cell Science 126(20), pp. 4627-4635. (10.1242/jcs.124917)
Midgley, A. C.et al. 2013. Transforming growth factor-β1 (TGF-β1)-stimulated fibroblast to myofibroblast differentiation Is mediated by hyaluronan (HA)-facilitated epidermal growth factor receptor (EGFR) and CD44 co-localization in lipid rafts. Journal of Biological Chemistry 288(21), pp. 14824-14838. (10.1074/jbc.M113.451336)
Francis, R. J., Kotecha, S. and Hallett, M. B. 2013. Ca2+ activation of cytosolic calpain induces the transition from apoptosis to necrosis in neutrophils with externalized phosphatidylserine. Journal of Leukocyte Biology 93(1), pp. 95-100. (10.1189/jlb.0412212)

2012

Adams, S. E.et al. 2012. Potent inhibition of Ca2+-dependent activation of calpain-1 by novel mercaptoacrylates. MedChemComm 3(5), pp. 566-570. (10.1039/c2md00280a)

2011

Brasen, J. C., Olsen, L. F. and Hallett, M. B. 2011. Extracellular ATP induces spikes in cytosolic free Ca2+ but not in NADPH oxidase activity in neutrophils. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1813(8), pp. 1446-1452. (10.1016/j.bbamcr.2011.05.002)
McDonald, J. U.et al. 2011. In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils. The FASEB Journal 25(6), pp. 1972-1982. (10.1096/fj.10-178517)
Dewitt, S. and Hallett, M. B. 2011. Optical complexities of living cytoplasm - implications for live cell imaging and photo-micromanipulation techniques. Journal of Microscopy 241(3), pp. 221-224. (10.1111/j.1365-2818.2010.03451.x)
Elumalai, G. L., Dewitt, S. and Hallett, M. B. 2011. Ezrin and talin relocates from the plasma membrane to cytosol during neutrophil extravasation [Abstract]. European Journal of Clinical Investigation 41(s1), pp. 47. (10.1111/j.1365-2362.2011.02508.x)
Lewis, K., Dewitt, S. and Hallett, M. B. 2011. The role of calpain translocation in neutrophil spreading [Abstract]. European Journal of Clinical Investigation 41(s1), pp. 47. (10.1111/j.1365-2362.2011.02508.x)
Francis, R. J., Kotecha, S. and Hallett, M. B. 2011. Ca2+ events in apoptosis and necrosis of neutrophils [Abstract]. European Journal of Clinical Investigation 41(s1), pp. 46-47. (10.1111/j.1365-2362.2011.02508.x)
Ishak, R., Dewitt, S. and Hallett, M. B. 2011. Calpain-1: investigating its role in murine neutrophils [Abstract]. European Journal of Clinical Investigation 41(s1), pp. 24. (10.1111/j.1365-2362.2011.02508.x)
Hallett, M. B. and Dewitt, S. 2011. A trick of the light: the optical properties of living cytoplasm which can mislead. Integrative Biology 3(3), pp. 180-184. (10.1039/c0ib00039f)

2010

Brasen, J. C., Dewitt, S. and Hallett, M. B. 2010. A reporter of UV intensity delivered to the cytosol during photolytic uncaging. Biophysical Journal 98(7), pp. L25-L27. (10.1016/j.bpj.2009.12.4271)
Brasen, J. C., Olsen, L. F. and Hallett, M. B. 2010. Cell surface topology creates high Ca2+ signalling microdomains. Cell Calcium 47(4), pp. 339-349. (10.1016/j.ceca.2010.01.005)

2009

Yang, J.et al. 2009. Low connexin channel-dependent intercellular communication in human adult hematopoietic progenitor/stem cells: Probing mechanisms of autologous stem cell therapy. Cell Communication and Adhesion 16(5-6), pp. 138-145. (10.3109/15419061003653763)
Dewitt, S., Darley, R. L. and Hallett, M. B. 2009. Translocation or just location? Pseudopodia affect fluorescent signals. Journal of Cell Biology 184(2), pp. 197-203. (10.1083/jcb.200806047)
Hallett, M. B.et al. 2009. Fantastic Ca2+ "z-waves" fade out quietly. Cell Calcium 46(1), pp. 85-86. (10.1016/j.ceca.2009.04.003)
Verma, V.et al. 2009. Perturbing plasma membrane hemichannels attenuates calcium signalling in cardiac cells and HeLa cells expressing connexins. European Journal of Cell Biology 88(2), pp. 79-90. (10.1016/j.ejcb.2008.08.005)

2008

Hallett, M. . B., Von Ruhland, C. J. and Dewitt, S. 2008. Chemotaxis and the cell surface-area problem [Letter]. Nature Reviews Molecular Cell Biology 9(8), pp. 662. (10.1038/nrm2419-c1)
Lennon, S. V.et al. 2008. Elevations in cytosolic free Ca2+ are not required to trigger apoptosis in human leukaemia cells. Clinical and Experimental Immunology 87(3), pp. 465-471. (10.1111/j.1365-2249.1992.tb03021.x)
Saltmarsh, E. J. and Hallett, M. B. 2008. Addressing controversy in localised Ca2+ signals in human neutrophils [Abstract]. European Journal of Clinical Investigation 38(S1), pp. 62. (10.1111/j.1365-2362.2008.01926.x)
Hallett, M. B. and Williams, A. S. 2008. Stopping the traffic: A route to arthritis therapy. European Journal of Immunology 38(10), pp. 2650-2653. (10.1002/eji.200838786)
Taneja, R.et al. 2008. Immature circulating neutrophils in sepsis have impaired phagocytosis and calcium signaling. Shock 30(6), pp. 618-622. (10.1097/SHK.0b013e318173ef9c)

2007

Dewitt, S. and Hallett, M. B. 2007. Leukocyte membrane 'expansion': a central mechanism for leukocyte extravasation. Journal of leukocyte biology 81(5), pp. 1160-1164. (10.1189/jlb.1106710)
Hillson, E. J., Dewitt, S. and Hallett, M. B. 2007. Optical methods for the measurement and manipulation of cytosolic free calcium in neutrophils. In: Quinn, M. T., DeLeo, F. R. and Bokoch, G. M. eds. Neutrophil Methods and Protocols. Methods in Molecular Biology Vol. 412. Totowa, NJ: Humana Press, pp. 125-137., (10.1007/978-1-59745-467-4_10)

2006

Hallett, M. B., Dewitt, S. and Tian, W. 2006. Localised PtdIns(3,4,5)P3 or PtdIns(3,4)P2 at the phagocytic cup is required for both phagosome closure and Ca2+ signalling in HL60 neutrophils. Journal of Cell Science 119(3), pp. 443-51. (10.1242/jcs.02756)

2004

Ayub, K.et al. 2004. Ca2+ influx shutdown in neutrophils induced by Fas (CD95) cross-linking. Immunology 112(3), pp. 454-460. (10.1111/j.1365-2567.2004.01899.x)
Clayton, A.et al. 2004. Adhesion and signaling by B cell-derived exosomes: the role of integrins. The FASEB Journal 18, pp. 977-979. (10.1096/fj.03-1094fje)

2003

Tian, W.et al. 2003. Exclusion of exogenous phosphatidylinositol-3,4,5-trisphosphate from neutrophil-polarizing pseudopodia: stabilization of the uropod and cell polarity. EMBO Reports 4(10), pp. 982-988. (10.1038/sj.embor.embor947)
Hallett, M. B., Laffafian, I. and Dewitt, S. 2003. Phagosomal oxidative activity during beta2 integrin (CR3)-mediated phagocytosis by neutrophils is triggered by a non-restricted Ca2+ signal: Ca2+ controls time not space. Journal of Cell Science 116(14), pp. 2857-65. (10.1242/jcs.00499)
Dewitt, S.et al. 2003. Cytosolic Ca2+ measurement and imaging in inflammatory cells. In: Winyard, P. G. and Willoughby, D. A. eds. Inflammation Protocols. Methods in Molecular Biology Vol. 255. Totowa, NJ: Humana Press, pp. 47-63., (10.1385/1-59259-374-7:47)
Hallett, M. B., Cole, C. and Dewitt, S. 2003. Detection and visualization of oxidase activity in phagocytes. In: Winyard, P. G. and Willoughby, D. A. eds. Inflammation Protocols., Vol. 225. Methods in Molecular Biology Totowa, NJ: Humana Press, pp. 61-67., (10.1385/1-59259-374-7:61)
Morris, M. R.et al. 2003. Phagocytosis by inflammatory phagocytes; experimental strategies for stimulation and quantification. In: Winyard, P. G. and Willoughby, D. A. eds. Inflammation Protocols. Methods in Molecular Biology Vol. 225. Totowa, NJ: Humana Press, pp. 35-46., (10.1385/1-59259-374-7:35)

2002

Hallett, M. B. and Dewitt, S. 2002. Cytosolic free Ca(2+) changes and calpain activation are required for beta integrin-accelerated phagocytosis by human neutrophils. The Journal of Cell Biology 159(1), pp. 181-9. (10.1083/jcb.200206089)
Brown, S. A.et al. 2002. Factor VIIa induced release of von Willebrand factor from human umbilical vein endothelial cells by a tyrosine kinase dependent pathway. Journal of Thrombosis and Haemostasis 87, pp. 1057-1061.
Hiscox, S. E.et al. 2002. GPI-anchored GFP signals Ca2+ but is homogeneously distributed on the cell surface. Biochemical and Biophysical Research Communications 293(2), pp. 714-721. (10.1016/S0006-291X(02)00280-2)

2001

Sathish, J. G.et al. 2001. Requirement for CD28 co-stimulation is lower in SHP-1-deficient T cells. European Journal of Immunology 31(12), pp. 3649-3658. (10.1002/1521-4141(200112)31:12<3649::AID-IMMU3649>3.0.CO;2-8)

1998

Pettit, E. J. and Hallett, M. B. 1998. Release of 'caged' cytosolic Ca2+ triggers rapid spreading of human neutrophils adherent via integrin engagement. Journal of Cell Science 111(15), pp. 2209-2215.
Pettit, E. J. and Hallett, M. B. 1998. NO DOI! Two distinct Ca2+ storage and release sites in human neutrophils. Journal of leukocyte biology 63(2), pp. 225-232.
Pettit, E. J. and Hallett, M. B. 1998. Ca2+ signalling delays in neutrophils: effects of prior exposure to platelet activating factor or formyl-met-leu-phe. Cellular Signalling 10(1), pp. 49-53. (10.1016/S0898-6568(97)00073-9)

1997

Jiang, W. G.et al. 1997. Regulation of desmosomal cell adhesion in human tumour cells by polyunsaturated fatty acids. Clinical and Experimental Metastasis 15(6), pp. 593-602. (10.1023/A:1018435229087)
Hallett, M. B. and Petit, E. J. 1997. The use of single image random dot stereograms for presenting 3D microscopic confocal images. Journal of Microscopy 186(3), pp. 275-278. (10.1046/j.1365-2818.1997.2130769.x)
Pettit, E. J., Davies, E. V. and Hallett, M. B. 1997. NO DOI! The microanatomy of calcium stores in human neutrophils: relationship of structure to function. Histology and Histopathology 12(2), pp. 479-490.
Hallett, M. B. 1997. Is "life" based on clockwork biology or quantum uncertainty?. Perspectives in Biology and Medicine 41(1), pp. 101-107. (10.1353/pbm.1997.0023)
Al-Mohanna, F., Pettit, E. J. and Hallett, M. B. 1997. Does actin polymerization status modulate Ca2+Storage in human neutrophils? Release and coalescence of Ca2+Stores by cytochalasins. Experimental Cell Research 234(2), pp. 379-387. (10.1006/excr.1997.3596)
Pettit, E. J. and Hallett, M. B. 1997. Pulsatile Ca2+Influx in human neutrophils undergoing CD11b/CD18 integrin engagement. Biochemical and Biophysical Research Communications 230(2), pp. 258-261. (10.1006/bbrc.1996.5931)
Hallett, M. B. and Pettit, E. J. 1997. Stochastic events underlie Ca2+Signalling in neutrophils. Journal of Theoretical Biology 186(1), pp. 1-6. (10.1006/jtbi.1996.0345)
Roberts, G.et al. 1997. The timing and magnitude of Ca2+ signaling by CD32 depends on its redistribution on the cell surface. Experimental Cell Research 230(2), pp. 303-309. (10.1006/excr.1996.3416)
Hallett, M. B. 1997. Controlling the molecular motor of neutrophil chemotaxis. Bioessays 19(7), pp. 615-621. (10.1002/bies.950190712)
Edwards, S. W. and Hallett, M. B. 1997. Seeing the wood for the trees: the forgotten role of neutrophils in rheumatoid arthritis. Immunology Today 18(7), pp. 320-324. (10.1016/S0167-5699(97)01087-6)
Davies, E. V., Roberts, G. M. and Hallett, M. B. 1997. Spatial and temporal separation of calcium signals in myeloid cells stimulated by immune complexes. Cellular Signalling 9(6), pp. 457-462. (10.1016/S0898-6568(97)00039-9)
Hiscox, S. E.et al. 1997. Expression of the HGF/SF Receptor, c-met, and its ligand in human colorectal cancers. Cancer Investigation 15(6), pp. 513-521. (10.3109/07357909709047592)

1996

Jones, J.et al. 1996. Effect of inhibitors of tyrosine kinase and phospholipase C on neutrophil response to perforin attack. Immunology 89(Supp1), pp. R187-R187.
Jiang, W. G.et al. 1996. Inhibition of membrane ruffling and ezrin translocation by gamma linolenic acid. International Journal of Oncology 9(2), pp. 279-284. (10.3892/ijo.9.2.279)
Pettit, E. J. and Hallett, M. B. 1996. Localised and global cytosolic Ca2+ changes in neutrophils during engagement of Cd11b/CD18 integrin visualised using confocal laser scanning reconstruction. Journal of Cell Science 109(7), pp. 1689-1694.
Jiang, W. G.et al. 1996. Inhibition of neutrophil respiratory burst and cytokine priming by γ-linolenic acid. British Journal of Surgery 83(5), pp. 659-664. (10.1002/bjs.1800830523)
Hallett, M. B., Petit, E. J. and Davies, E. V. 1996. Capacitative Ca2+ influx and a diffusible influx factor [letter]. Biochemical Journal -London- 314(pt 3), pp. 1054-1056.
Hallett, M. B., Pettit, E. J. and Davies, E. F. 1996. Capacitative Ca2+ influx and a diffusible influx factor. Biochemical Journal 314(3), pp. 1054-1056.
Jiang, W. G.et al. 1996. Gamma linolenic acid inhibits tyrosine phosphorylation of focal adhesion kinase and paxillin and tumour cell matrix interaction. International Journal of Oncology 8(3), pp. 583-587. (10.3892/ijo.8.3.583)
Davies, E. V. and Hallett, M. B. 1996. Near membrane Ca2+ changes in neutrophils. Biochemical Society Transactions 24(1), pp. 92S-92S.
Lloyds, D., Neville, N. and Hallett, M. B. 1996. Tyrosine phosphorylation in inflammatory neutrophils. Presented at: Biochemical Society transactions 656th meeting, University College Dublin, 11–15 September 1995Biochemical Society transactions, Vol. 24. 656th Meeting Dublin Portland Press pp. 79S-79S., (10.1042/bst024079s)
Pettit, E. J. and Hallett, M. B. 1996. Integrin triggered Ca2+ signalling in human neutrophils. Presented at: Biochemical Society Transactions 656th Meeting, University College Dublin, 11–15 September 1995Biochemical Society Transactions, Vol. 24. Biochemical Society Transactions 656th Meeting Portland Press pp. 70S-70S., (10.1042/bst024070s)
Jiang, W. G.et al. 1996. Hepatocyte growth factor induces tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin and enhances cell-matrix interactions. Oncology Reports 3(5), pp. 819-823. (10.3892/or.3.5.819)
Pettit, E. J. and Hallett, M. B. 1996. Temporal and spatial resolution of Ca2+Release and influx in human neutrophils using a novel confocal laser scanning mode. Biochemical and Biophysical Research Communications 229(1), pp. 109-113. (10.1006/bbrc.1996.1765)
Lloyds, D.et al. 1996. Tyrosine phosphorylation in neutrophils from synovial fluid of patients with rheumatoid arthritis. Rheumatology 35(9), pp. 846-852. (10.1093/rheumatology/35.9.846)
Davies, E. V. and Hallett, M. B. 1996. Near membrane Ca2+ changes resulting from store release in neutrophils: detection by FFP-18. Cell Calcium 19(4), pp. 355-362. (10.1016/S0143-4160(96)90076-7)
Han, Z.et al. 1996. Inhibition of motility and invasion of human lung cancer cells by invasion inhibiting factor 2. Surgical Oncology 5(2), pp. 77-84. (10.1016/S0960-7404(96)80004-1)
Hallett, M. B., Davies, E. V. and Pettit, E. J. 1996. Fluorescent methods for measuring and imaging the cytosolic free Ca2+ in neutrophils. Methods: A Companion to Methods in Enzymology 9(3), pp. 591-606.

1995

Jiang, W. G.et al. 1995. Regulation of the expression of E-cadherin on human cancer cells by gamma-linolenic acid (GLA). Cancer Research 55, pp. 5043-5048.
Jiang, W. G.et al. 1995. Gamma linolenic acid up-regulates e-cadherin expression in human cancer cells. British Journal of Surgery 82(11), pp. 1565-1565.
Salaman, R. A.et al. 1995. Compression Therapy reduces neutrophil priming in venous ulcer disease. British Journal of Surgery 82(11), pp. 1553-1554.
Jiang, W. G.et al. 1995. Expression of catenins in human cancer cells and its regulation by n-6 polyunsaturated fatty acids. Anticancer Research 15(6B), pp. 2569-2573.
Jiang, W. G.et al. 1995. NO DOI! Regulation of the expression of E-cadherin on human cancer cells by gamma-linolenic acid (GLA). Cancer Research 55(21), pp. 5043-5048.
Jiang, W. G.et al. 1995. Inhibition of HGF/SF-induced membrane ruffling and cell motility by transient elevation of cytosolic free Ca2+. Experimental Cell Research 220(2), pp. 424-433. (10.1006/excr.1995.1334)
Laffafian, I. and Hallett, M. B. 1995. Does cytosolic free Ca2+ signal neutrophil chemotaxis in response to formylated chemotactic peptide?. Journal of Cell Science 108(10), pp. 3199-3205.
Davies, E. V. and Hallett, M. B. 1995. A novel pathway for Ca2+ signalling in neutrophils by immune complexes. Immunology 85(4), pp. 538-543.
Jiang, W. G.et al. 1995. Regulation of motility and invasion of cancer cells by human monocytic cells. Anticancer Research 15(4), pp. 1303-1310.
Roberts, G. M., Davies, E. V. and Hallett, M. B. 1995. Slow Ca2+ waves in large myeloid cells as a result of a diffusible cytosolic factor. Journal of leukocyte biology 57(6), pp. 837-841.
Jiang, W. G.et al. 1995. Inhibition of HGF/SF-induced tumor-cell membrane ruffling by calcium signalling. British Journal of Surgery 82(5), pp. 682-683.
Van Den Berg, C. W.et al. 1995. Exogenous CD59 incorporated into U937 cells through its glycosyl phosphatidylinositol anchor becomes associated with signalling molecules in a time dependent manner. Biochemical Society Transactions 23(2), pp. 269S. (10.1042/bst023269s)
Laffafian, I.et al. 1995. Complement component C9-dependent cytosolic free Ca2+ rise and recovery in neutrophils. Cell Calcium 17(4), pp. 279-286. (10.1016/0143-4160(95)90074-8)
Jiang, W. G.et al. 1995. Inhibition of hepatocyte growth factor-induced motility and in vitro invasion of human colon cancer cells by gamma-linolenic acid. British Journal of Cancer 71(4), pp. 744-752. (10.1038/bjc.1995.145)
Lloyds, D., Brindle, N. P. and Hallett, M. B. 1995. Priming of human neutrophils by tumour necrosis factor-alpha and substance P is associated with tyrosine phosphorylation. Immunology 84(2), pp. 220-226.
Davies, E. V. and Hallett, M. B. 1995. A soluble cellular factor directly stimulates Ca2+ entry in neutrophils. Biochemical and Biophysical Research Communications 206(1), pp. 348-354. (10.1006/bbrc.1995.1048)
Lloyds, D. and Hallett, M. B. 1995. Development of oxidase 'priming' in maturing HL60 cells: Correlation with protein expression and tyrosine phosphorylation. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1267(1), pp. 65-71. (10.1016/0167-4889(95)00031-M)
Roberts, G. M., Hoy, T. and Hallett, M. B. 1995. The production of large 'signalling competent' myeloid cells from circulating CD34+ cells in neonatal blood. Journal of Immunological Methods 179(2), pp. 187-192. (10.1016/0022-1759(94)00284-4)
Hallett, M. B. and Lloyds, D. 1995. Neutrophil priming: the cellular signals that say 'amber' but not 'green'. Immunology Today 16(6), pp. 264-268. (10.1016/0167-5699(95)80178-2)
Hiscox, S.et al. 1995. Inhibition of cancer cell motility and invasion by interleukin-12. Clinical & Experimental Metastasis 13(5), pp. 396-404. (10.1007/BF00121916)
van den Berg, C. W.et al. 1995. Exogenous glycosyl phosphatidylinositol-anchored CD59 associates with kinases in membrane clusters on U937 cells and becomes Ca(2+)-signaling competent. Journal of cell biology 131(3), pp. 669-677. (10.1083/jcb.131.3.669)
Jiang, W. G.et al. 1995. Induction of tyrosine phosphorylation and translocation of ezrin by hepatocyte growth factor/scatter factor (HGF/SF). Biochemical and Biophysical Research Communications 217(3), pp. 1062-1069. (10.1006/bbrc.1995.2877)
Lloyds, D. and Hallett, M. B. 1995. Is raf-1 the 74 kDa signalling molecule in neutrophils?. Biochemical Journal 308(3), pp. 1037. (10.1042/bj3081037)
Pettit, E. J. and Hallett, M. B. 1995. Early Ca2+signalling events in neutrophils detected by rapid confocal laser scanning. Biochemical Journal 310(2), pp. 445-448. (10.1042/bj3100445)

1994

PETTIT, E. and Hallett, M. 1994. Neutrophil activation and priming during engagement of CD11b/CD18 integrins. Biochemical Society Transactions 22(3), pp. 327S. (10.1042/bst022327s)
Davies, E. V.et al. 1994. Altered Ca2+ signalling in human neutrophils from inflammatory sites. Annals of the Rheumatic Diseases 53(7), pp. 446-449. (10.1136/ard.53.7.446)
Jiang, W.et al. 1994. Induction of motility and invasion by interleukin-3 on human colonic-cancer cells. British Journal of Surgery 81(5), pp. 761-761.
Lloyds, D. and Hallett, M. B. 1994. Protein expression and development of oxidase "priming" in maturing HL60 cells. Biochemical Society Transactions 22(3), pp. 326S-326S. (10.1042/bst022326s)
Davies, E. V., Campbell, A. K. and Hallett, M. B. 1994. Ca2+ oscillations in neutrophils triggered by immune complexes result from Ca2+ influx. Immunology 82(1), pp. 57-62.
Roberts, G., Davies, E. V. and Hallett, M. B. 1994. Slow calcium waves imaged in myeloid cells derived from neonatal cord blood. Journal of leukocyte biology 55(4), pp. 461-466.
Jiang, W. G., Puntis, M. C. A. and Hallett, M. B. 1994. Neutrophil priming by cytokines in patients with obstructive jaundice.. HPB Surgery 7(4), pp. 281-289. (10.1155/1994/74202)
Jiang, W. G., Puntis, M. C. A. and Hallett, M. B. 1994. Neutrophil priming by cytokines in patients with obstructive jaundice. HPB Surgery 7(4), pp. 281-289. (10.1155/1994/74202)
Whiston, R. J.et al. 1994. Inappropriate neutrophil activation in venous disease. British Journal of Surgery 81(5), pp. 695-698. (10.1002/bjs.1800810522)
Lloyds, D. and Hallett, M. B. 1994. Neutrophil 'priming' induced by orthovanadate: Evidence of a role for tyrosine phosphorylation. Biochemical Pharmacology 48(1), pp. 15-21. (10.1016/0006-2952(94)90218-6)
Jiang, W. G., Puntis, M. C. A. and Hallett, M. B. 1994. Molecular and cellular basis of cancer invasion and metastasis: Implications for treatment. British Journal of Surgery 81(11), pp. 1576-1590. (10.1002/bjs.1800811107)

1993

Lloyds, D. and Hallett, M. B. 1993. Tyrosine phosphorylation of proteins during "priming" of neutrophils. Biochemical Society Transactions 21(4), pp. 436S-436S. (10.1042/bst021436s)
Petersen, M., Williams, J. D. and Hallett, M. B. 1993. Cross-linking of CD11b or CD18 signals release of localized Ca2+ from intracellular stores in neutrophils. Immunology 80(1), pp. 157-159.
Mills, L.et al. 1993. Beta-adrenergic mobilization of Ca2+ from an intracellular store in rat submandibular acini. Biochemical Journal 293(3), pp. 691-695.
Jiang, W. G.et al. 1993. Regulation of spreading and growth of colon cancer cells by hepatocyte growth factor.. Clinical and Experimental Metastasis 11(3), pp. 235-242. (10.1007/BF00121166)
Morgan, B. P.et al. 1993. Cross-linking of CD59 and of other glycosyl phosphatidylinositol-anchored molecules on neutrophils triggers cell activation via tyrosine kinase. European Journal of Immunology 23(11), pp. 2841-2850. (10.1002/eji.1830231118)
Lloyds, D. and Hallett, M. B. 1993. Activation and priming of the human neutrophil oxidase response by substance P: Distinct signal transduction pathways. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1175(2), pp. 207-213. (10.1016/0167-4889(93)90024-J)
Jiang, W. G., Puntis, M. C. A. and Hallett, M. B. 1993. Monocyte-conditioned media possess a novel factor which increases motility of cancer cells. International Journal of Cancer 53(3), pp. 426-431. (10.1002/ijc.2910530313)
Jiang, W. G.et al. 1993. Regulation of spreading and growth of colon cancer cells by hepatocyte growth factor. Clinical & Experimental Metastasis 11(3), pp. 235-242. (10.1007/BF00121166)
Jiang, W. G., Hallett, M. B. and Puntis, M. C. A. 1993. Hepatocyte growth factor/scatter factor, liver regeneration and cancer metastasis. British Journal of Surgery 80(11), pp. 1368-1373. (10.1002/bjs.1800801104)

1992

Assender, J. W.et al. 1992. Inhibition of proliferation, but not of Ca2+ mobilization, by cyclic AMP and GMP in rabbit aortic smooth-muscle cells. Biochemical Journal 288(2), pp. 527-532.
Jiang, W. G.et al. 1992. Neutrophil priming by hepatocyte growth factor, a novel cytokine. Immunology 77(1), pp. 147-149.
Jiang, W. G., Puntis, M. and Hallett, M. B. 1992. U937 cells stimulated with opsonised zymozan particles provide a convenient laboratory source of tumour necrosis factor α. Journal of Immunological Methods 152(2), pp. 201-207. (10.1016/0022-1759(92)90141-F)
Davies, E. V., Campbell, A. K. and Hallett, M. B. 1992. Dissociation of store release from transmembrane influx of calcium in human neutrophils. FEBS Letters 313(2), pp. 121-125. (10.1016/0014-5793(92)81426-M)

1991

Jones, J.et al. 1991. Imaging Ca2+ changes in individual oligodendrocytes attacked by T-cell perforin. Immunology 74(4), pp. 572-577.
Davies, E. V., Hallett, M. B. and Campbell, A. K. 1991. Localized superoxide release by neutrophils can be provoked by a cytosolic calcium 'cloud'. Immunology 73(2), pp. 228-234.
Davies, E. V., Campbell, A. K. and Hallett, M. B. 1991. Synchronous free Ca2+ changes in individual neutrophils stimulated by leukotriene B4. FEBS Letters 291(1), pp. 135-138. (10.1016/0014-5793(91)81121-N)
Laffafian, I., Brown, R. C. and Hallett, M. B. 1991. The production of an amine-modified derivative of 5-aminosalicylic acid by activated neutrophils Roles for myeloperoxidase and chloride ions. Biochemical Pharmacology 42(10), pp. 1869-1874. (10.1016/0006-2952(91)90583-Q)
Davies, E. V.et al. 1991. Single cell imaging reveals abnormal intracellular calcium signals within rheumatoid synovial neutrophils. Rhuematology 30(6), pp. 443-448. (10.1093/rheumatology/30.6.443)

1990

Petersen, M.et al. 1990. Zymosan-induced leukotriene B4 generation by human neutrophils is augmented by rhTNF-alpha but not chemotactic peptide. Immunology 70(1), pp. 75-81.
Jones, J., Hallett, M. B. and Morgan, B. P. 1990. Reversible cell damage by T-cell perforins. Calcium influx and propidium iodide uptake into K562 cells in the absence of lysis. Biochemical Journal 267(2), pp. 303-307.
al-Mohanna, F. A. and Hallett, M. B. 1990. NO DOI!! Actin polymerization in neutrophils is triggered without a requirement for a rise in cytoplasmic Ca2+. Biochemical Journal 266(3), pp. 669-674.
Hallett, M. B., Dormer, R. L. and Campbell, A. K. 1990. Cytoplasmic free calcium: measurement and manipulation in living cells. In: Siddle, K. and Hutton, J. C. eds. Peptide hormones: a practical approach. Practical Approach Series Vol. 71. Oxford: IRL Press, pp. 115-150.
Williams, J. G., Hughes, L. E. and Hallett, M. B. 1990. Toxic oxygen metabolite production by circulating phagocytic cells in inflammatory bowel disease. Gut 31(2), pp. 187-193. (10.1136/gut.31.2.187)
Al-Mohanna, F. and Hallett, M. B. 1990. 'Clamping' actin in polymerized form in electropermeabilized neutrophils inhibits oxidase activation. Biochemical and Biophysical Research Communications 169(3), pp. 1222-1228. (10.1016/0006-291X(90)92027-W)
Hallett, M. B., Davies, E. V. and Campbell, A. K. 1990. Oxidase activation in individual neutrophils is dependent on the onset and magnitude of the Ca2+ signal. Cell Calcium 11(10), pp. 655-663. (10.1016/0143-4160(90)90020-U)

1989

Hallett, M. B. 1989. The unpredictability of cellular behavior: trivial or of fundamental importance to cell biology?. Perspectives in Biology and Medicine 33(1), pp. 110-119. (10.1353/pbm.1990.0041)
Williams, J. and Hallett, M. B. 1989. The reaction of 5-amino-salicylic acid with hypochlorite. Biochemical Pharmacology 38(1), pp. 149-154. (10.1016/0006-2952(89)90161-5)
Williams, J. G. and Hallett, M. B. 1989. Effect of sulphasalazine and its active metabolite, 5-amino-salicylic acid, on toxic oxygen metabolite production by neutrophils. Gut 30(11), pp. 1581-1587. (10.1136/gut.30.11.1581)
Srivastava, E. D., Hallett, M. B. and Rhodes, J. 1989. Effect of nicotine and cotinine on the production of oxygen free radicals by neutrophils in smokers and non-smokers. Human & Experimental Toxicology 8(6), pp. 461-463. (10.1177/096032718900800605)
Cooke, E., Al-Mohanna, F. A. and Hallett, M. B. 1989. Ca2+-dependent and independent mechanisms in neutrophil activation; roles of kinase C, diacylglycerol, and unidentified intracellular messengers. In: Hallett, M. B. ed. The neutrophil; cellular biochemistry and physiology. Boca Raton: CRC Press, pp. 219-242.

1988

Williams, J. G. and Hallett, M. B. 1988. Increased production of oxygen radicals by circulating monocytes in inflammatory bowel-disease evidence of activation. British Journal of Surgery 75(12), pp. 1269-1269.
Zhang, L., Roberts, G. M. and Hallett, M. . B. 1988. Diphenyliodonium-sensitive killing of K652 tumor-cells by blood mononuclear cells. Biochemical Society Transactions 16(5), pp. 902-902.
Williams, J. G. and Hallett, M. B. 1988. Flourimetric determination of hypochlorite scavenging by 5amino salicyclic-acid (5ASA). Gut 29(10), pp. A1437-A1437.
Williams, J. G. and Hallett, M. B. 1988. The mode of action of sulphasalazine and 5-aminosalicyclic acid-inhibition of oxygen radical production by neutrophils. British Journal of Surgery 75(6), pp. 600-601.
Al-Mohanna, F. A. and Hallett, M. B. 1988. The use of fura-2 to determine the relationship between cytoplasmic free Ca2+ and oxidase activation in rat neutrophils. Cell Calcium 9(1), pp. 17-26. (10.1016/0143-4160(88)90034-6)

1987

Patel, A. K., Hallett, M. B. and Campell, A. K. 1987. Threshold responses in production of reactive oxygen metabolites in individual neutrophils detected by flow cytometry and microfluorimetry. Biochemical Journal 248(1), pp. 173-180.
Al-Mohanna, F. and Hallett, M. B. 1987. Actin polymerization modifies stimulus-oxidase coupling in rat neutrophils. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 927(3), pp. 366-371. (10.1016/0167-4889(87)90101-7)
Al-Mohanna, F., Ohishi, I. and Hallett, M. B. 1987. Botulinum C2 toxin potentiates activation of the neutrophil oxidase Further evidence of a role for actin polymerization. FEBS Letters 219(1), pp. 40-44. (10.1016/0014-5793(87)81187-0)
Cooke, E., Al-Mohanna, F. A. and Hallett, M. B. 1987. Diacylglycerol kinase inhibitor, R59022, potentiates neutrophil oxidase activation by Ca2+-dependent stimuli. Biochemical Pharmacology 36(20), pp. 3459-3462. (10.1016/0006-2952(87)90326-1)

1986

Horgan, K.et al. 1986. Tamoxifen inhibition of neutrophil oxidase activation - a mechanism of antitumor action. British Journal of Surgery 73(6), pp. 492-492.
Horgan, K.et al. 1986. Tamoxifen inhibition of protein kinase C. European Journal of Cancer and Clinical Oncology 22(6), pp. 731-731. (10.1016/0277-5379(86)90233-6)
Cooke, E. and Hallett, M. B. 1986. Retinoids interact with the mechanism of neutrophil oxidase activation. Biochemical Society Transactions 14(5), pp. 954-955.
Shandall, A. A.et al. 1986. Colonic healing: A role for polymorphonuclear leucocytes and oxygen radical production. British Journal of Surgery 73(3), pp. 225-228. (10.1002/bjs.1800730325)
Lowndes, R. H. and Hallett, M. B. 1986. A versatile light microscope heating stage for biological temperatures. Journal of Microscopy 142(3), pp. 371-374. (10.1111/j.1365-2818.1986.tb04292.x)
Horgan, K.et al. 1986. Inhibition of protein kinase C mediated signal transduction by tamoxifen. Biochemical Pharmacology 35(24), pp. 4463-4465. (10.1016/0006-2952(86)90764-1)
Hallett, M. B., Edwards, S. W. and Campbell, A. K. 1986. Control of oxygen production by polymorphonuclear leukocytes monitored by luminol-dependent chemiluminescence: the roles of intracellular calcium, oxygen concentration and redox components. In: Van Dyke, K. ed. Cellular chemiluminescence. Boca Raton: CRC Press, pp. 173-192.

1985

Cooke, E. and Hallett, M. B. 1985. NO DOI!! The role of C-kinase in the physiological activation of the neutrophil oxidase. Evidence from using pharmacological manipulation of C-kinase activity in intact cells. Biochemical Journal 232(2), pp. 323-327.
Cooke, E., Almohanna, F. and Hallett, M. B. 1985. The role of microfilaments in polymorphonuclear leukocyte oxidase activation. Biochemical Society Transactions 13(6), pp. 1173-1174. (10.1042/bst0131173)
Roberts, P. A.et al. 1985. Inhibition by adenosine of reactive oxygen metabolite production by human polymorphonuclear leucocytes. Biochemical Journal 227(2), pp. 669-674.
Campbell, A. K., Dormer, R. L. and Hallett, M. B. 1985. Coelenterate photoproteins as indicators of cytoplasmic free Ca2+ in small cells. Cell Calcium 6(1-2), pp. 69-82. (10.1016/0143-4160(85)90035-1)
Hallett, M. B., Shandall, A. and Young, H. L. 1985. Mechanism of protection against ?reperfusion injury? by aprotinin Roles of polymorphonuclear leucocytes and oxygen radicals. Biochemical Pharmacology 34(10), pp. 1757-1761. (10.1016/0006-2952(85)90646-X)
Hallett, M. B. 1985. Quantal secretion and response lag demonstrated in single rat neutrophils. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 847(1), pp. 15-19. (10.1016/0167-4889(85)90146-6)
Matthews, S. B.et al. 1985. No DOI! The adrenochrome pathway. A potential catabolic route for adrenaline metabolism in inflammatory disease. Advances in myocardiology 6, pp. 367-381.
Campbell, A. K., Hallett, M. B. and Weeks, I. 1985. Chemiluminescence as an analytical tool in cell biology and medicine. In: Glick, D. ed. Methods in biochemical analysis., Vol. 31. New York: Wiley, pp. 317-416., (10.1002/9780470110522.ch7)
Dormer, R. L., Hallett, M. B. and Campbell, A. K. 1985. Measurement of intracellular free calcium: Importance during activation and injury of small cells. In: Parrat, J. R. ed. Control and manipulation of calcium movements: a Biological Council symposium. New York: Raven Press, pp. 1-27.

1984

Edwards, S. W., Hallett, M. B. and Campbell, A. K. 1984. Oxygen-radical production during inflammation may be limited by oxygen concentration. Biochemical Journal 217(3), pp. 851-854.
Hallett, M. B. and Campbell, A. K. 1984. Is intracellular Ca2+ the trigger for oxygen radical production by polymorphonuclear leucocytes?. Cell Calcium 5(1), pp. 1-19. (10.1016/0143-4160(84)90150-7)

1983

Hallett, M. B. and Campbell, A. K. 1983. Direct measurement of intracellular free Ca2+ in rat peritoneal macrophages: correlation with oxygen-radical production. Immunology 50(3), pp. 487-495.
Hallett, M. B. and Campbell, A. K. 1983. Two distinct mechanisms for stimulation of oxygen-radical production by polymorphonuclear leucocytes. Biochemical Journal 216(2), pp. 459-465.
Campbell, A. K. and Hallett, M. B. 1983. Measurement of intracellular calcium ions and oxygen radicals in polymorphonuclear leucocyte-erythrocyte ‘ghost’ hybrids. The Journal of Physiology 338(1), pp. 537-550. (10.1113/jphysiol.1983.sp014688)
Edwards, S. W.et al. 1983. Decrease in apparent Km for oxygen after stimulation of respiration of rat polymorphonuclear leukocytes. FEBS Letters 161(1), pp. 60-64. (10.1016/0014-5793(83)80730-3)

1982

Hallett, M. B. and Campbell, A. 1982. Applications of coelenterate luminescent protein.. In: Kricka, L. J. and Clark, T. J. eds. Clinical and biochemical luminescence. New York: Marcel Dekker, pp. 89-133.

1979

Campbell, A. K. and Hallett, M. B. 1979. Luminescence in cells and vesicles isolated from the hydroid Obelia geniculata [proceedings]. The Journal of Physiology 287, pp. 4P-5P.

1978

Dormer, R. L., Hallett, M. B. and Campbell, A. K. 1978. The incorporation of the calcium-activated photoprotein into isolated rat fat-cells by liposome-cell fusion [proceedings]. Biochemical Society Transactions 6(3), pp. 570-572.

1977

Foreman, J. C., Hallett, M. B. and Mongar, J. L. 1977. The relationship between histamine secretion and 45 calcium-uptake by mast cells. The Journal of Physiology 271(1), pp. 193-214. (10.1113/jphysiol.1977.sp011996)
Foreman, J. C., Hallett, M. B. and Mongar, J. L. 1977. Movement of strontium ions into mast cells and its relationship to the secretory response. The Journal of Physiology 271(1), pp. 233-251. (10.1113/jphysiol.1977.sp011998)
Foreman, J. C., Hallett, M. B. and Mongar, J. L. 1977. Site of action of the antiallergic drugs cromoglycate and doxantrazole [proceedings]. British Journal of Pharmacology 59(3), pp. 473P-474P.
Foreman, J. C., Hallett, M. B. and Mongar, J. L. 1977. Strontium ions as a probe for the role of alkaline-earth metal ions in histamine secretion [proceedings]. Biochemical Society Transactions 5(4), pp. 879-883.

1975

Foreman, J. C., Hallett, M. B. and Mongar, J. L. 1975. Proceedings: 45-Calcium uptake in rat peritoneal mast cells. British Journal of Pharmacology 55(2), pp. 283P-284P.

i) Cell Signalling series of lecturers (3) – BSc (Hons)

(ii) Core course Cell Signalling (3) BSc Intercalated

(iii) Phagocytes, function, signalling and pathology (3) BSc and BSc Intercalated

(iv) Neutrophilopathies

The neutrophil represents an unusual cell type within the body, in which there is a degree of autonomy of cell-response decision-making. In some respects, the possible response outcomes are “primitive” cell behavioural responses, e.g. cell shape change, phagocytosis and chemotaxis, whereas in other respects there are more sophisticated outcomes, e.g. apoptosis, gene expression and oxidase assembly. Individual neutrophils must perform a program of activity in the correct sequence, location and timing (based on their repertoire of behaviours) to produce effective anti-bacterial protection. Yet, many of the same steps lead to pathological and chronic inflammatory diseases, such as rheumatoid arthritis.

The goal of our research is to understand the signalling and integration of these separate behaviours. While this is a worthy goal in itself, as it contributes to our fundamental knowledge of cellular processes; it underpins the larger aim of understanding and ultimately preventing the aberrant behaviour of these cells during inflammatory diseases. At present, the Group has focused on three aspects of neutrophil signalling (i) cytosolic free Ca2+ signals (ii) PIP2 generation and signalling and (ii) calpain translocation and activation. We have built up the necessary equipment for measurement, manipulation and imaging chemical changes in individual living neutrophils, and solved some of the difficult problems which arise when working with these cells.