The functional proteome of the microbiome and its role in endotoxin modulation
Inflammatory disease such as Inflammatory Bowel Disease and Non-alcoholic fatty liver disease, are all increasing in the global population, as are many other non-communicable diseases.
In both the former diseases there is role for the microbiome and how it interacts with the host’s innate immunity. One such interaction is via endotoxins from the microbiome interacting with the host’s colonocytes and hepatocytes to drive an inflammatory response. However, we believe that the microbiome is not only responsible for making the endotoxin, but also in controlling the levels of endotoxin and exposure that the colonocytes and hepatocytes experience. Hence our novel hypothesis is that the microbiome’s proteases and phosphatases are a key factor in controlling levels of endotoxin and thus exposure in the gut and liver. Importance: Current systems biology projects consistently fail to integrate the microbiome into inflammatory studies. This project will undertake an in vitro investigation of the bacterial proteases and bacterial alkaline phosphatases and how they interact with different classes of endotoxins to determine their role in inflammatory processes and thus provide more insight.