Detection of outer membrane vesicles (OMVs) in common gut bacteria and fecal samples

The role of bacteria and microbes in cancer has received considerable attention due to the involvement of Helicobacter pylori and viruses such as HPV, EBV and Hep B and C.

However, a new group of bacteria are being considered as drivers of cancer and in particular colorectal cancer (CRC), these are the gut commensal bacteria. In recent years we have started to better understand the ecology of gut bacteria in CRC, but we are still unclear of the mechanisms, but inflammation does seem to be a risk factor for developing CRC. Several factors have been proposed, proteases, endotoxins and genotoxins (e.g. colibactin). Recently outer membrane vesicles or OMVs, which derive from Gram negative bacteria, have also been implicated. In this study we wish to determine whether outer membrane vesicles are found in the healthy gut and from Gram negative bacteria in the gut. If they do, we wish to know at what levels they exist and whether they can induce cell damage in tissue culture and 3D-organoids.

Supervisors

Professor Julian Marchesi

Professor Julian R Marchesi

Professor of Human Microbiome Research at Cardiff University and Reader in Digestive Health at Imperial College London

Email:
marchesijr@cardiff.ac.uk
Telephone:
+44 (0)29 2087 4188

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