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Zeiss PALM MicroBeam Laser Micro-dissector

The PALM Micro Beam is a state of the art, laser micro-dissection system for isolation of DNA, RNA and protein from histological sections (paraffin wax or cryo) or live cells.

Make/model Zeiss PALM MicroBeam laser micro-dissector
Details Fully motorised Zeiss Axio Observer microscope with brightfield and epifluorescence. RoboMover automated collection device. Frequency tripled solid state 355nm laser Zeiss AxioCam colour digital camera
Facility Bioimaging Research Hub
School School of Biosciences

The instrument is fully motorised with  x5, x20, x40, x63 objective lenses and RGB filters for epifluorescence. It is equipped with a Zeiss AxioCam colour digital camera and RoboMover for automated capture and collection of laser micro-dissected samples. system is fully integrated via Zeiss RoboSoftware.

Objectives

  • 10x/0.50 Fluar
  • 20x/0.40 LD Plan Neofluar
  • 40x/0.60 LD Plan Neofluar
  • 63x/0.75 LD Plan-Neofluar

Laser

  • Frequency tripled solid state 355nm laser

Internal users can book the equipment using the shared Outlook calendar ‘BIOSI – HEB Zeiss PALM laser microdissector’.

Find out how to book equipment through Outlook.

Please keep to your allotted booking time. You will be charged for the time that you have reserved the instrument for, irrespective of usage. The minimum booking time is 1 hour.

Cancellations should be made at least 24 hours in advance. If you fail to turn up for a booking you will be charged.

Please contact Mark Bishop (bishopm@cardiff.ac.uk; +44(0)29 2068 8512) for details of costs.

All users must be trained and registered by Bioimaging Unit staff before using any of the instruments within the Unit.

Please contact Mark Bishop (bishopm@cardiff.ac.uk; +44(0)29 2068 8512).

To comply with Cardiff University Health and Safety Policy, individuals must receive appropriate training before using this equipment and be aware of the risks involved in its use.

All users are expected to observe the following. If they fail to comply they will lose their access rights:

  • Food and drink are not permitted within the facility.
  • All waste material (including laboratory gloves) should be properly disposed of outside of the facility.
  • Coats and bags when left on the floor in a low light environment represent a tripping hazard. Either hang them on the internal door hooks or, better still, do not bring them into the imaging suites.
  • Do not use any equipment that you have not been properly trained for.  All users should familiarise themselves with the appropriate risk assessments for the procedures being undertaken.
  • All USB data storage devices must be virus checked before plugging them into any of our systems.
  • All systems should be correctly powered down at the end of your session and usage correctly logged in the database.

Get in touch

Mr Mark Bishop

Email
bishopm@cardiff.ac.uk
Telephone
+44 (0)29 2088 8512

Location

Lab 1.27
Hadyn Ellis Building
Maindy Road
CF24 4HQ

Opening hours

Monday-Friday 09:00-17:00. Users will require proximity card access rights

Resources

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