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Dr Stephen R Clark 


RESEARCH

I am a Lecturer in Immunopharmacology at the School of Pharmacy & Pharmaceutical Sciences, Cardiff University, where I run a laboratory focused on innate cellular immune defences against invading microorganisms.  

My interests include anti-microbial defences of neutrophils and the neutrophil extracellular traps (NETs) that they produce.  NETs are formed when activated neutrophils release large web-like structures of DNA decorated with granule proteins.  Formation of NETs is an important protective anti-microbial response to bacteria, fungi and viruses but occurs at the expense of tissue injury.  I am particular keen to understand how the formation of NETs is controlled and limited during many forms of neutrophil activation.

Member of the Critical Illness Research Group

clarke research imageNeutrophil extracellular traps (NETs) formed by neutrophils from a healthy volunteer after activation in the laboratory.  Fluorescent image of NETs stained with a DNA dye taken at x65 magnification – scale bar 10 µm.

Neutrophil extracellular traps (NETs) formed by neutrophils from a healthy volunteer after activation in the laboratory.  Fluorescent image of NETs stained with a DNA dye taken at x65 magnification – scale bar 10 µm.

 

Research Funding

Current

  • Intensive Care Foundation
  • Royal Society

Completed Projects

  • Wellcome Trust
  • Marie Curie, European Commission FP7
  • British Lung Foundation
  • Heart & Stroke Foundation of Canada
  • Canadian Institutes for Health Research Training Program
  • British Heart Foundation

 

Key Publications

Wang ECY, Newton Z, Hayward OA, Clark SR, Collins F, Perks WV, Singh RK, Twohig JP & Williams AS. (2014) Regulation of early cartilage destruction in inflammatory arthritis by Death Receptor 3.  Arthritis & Rheumatology, 66 (10), 2762-72

Thomas CP, Clark SR, Hammond VJ, Aldrovandi M, Collins PW, O’Donnell VB. (2014) Identification and quantitation of aminophospholipid molecular species on the surface of apoptotic and activated cells. Nature Protocols (2014) vol. 9 (1) pp. 51-63

Clark SR, Thomas CP, Hammond VJ, Aldrovandia M, Wilkinson GWG, Hart KW, Murphy RC, Collins PW & O’Donnell VB. (2013) Characterization of Platelet Aminophospholipid Externalization Reveals Fatty Acids as Molecular Determinants that Regulate Coagulation. PNAS 110(15) 5875-80.

Lauder SN, Taylor PR, Clark SR, Evans RL, Hindley JP, Smart K, Leach H, Kidd EJ, Broadley KJ, Jones SA, Wise MP, Godkin AJ, O’Donnell VB & Gallimore AW. (2011) Paracetamol Reduces Influenza-Induced Immunopathology without Compromising Virus Clearance or the Generation of Protective Immunity. Thorax 66(5) 368-74

Clark SR, Guy CJ, Scurr MJ, Taylor PR, Kift-Morgan AP, Hammond VJ, Thomas CP, Coles B, Roberts GW, Eberl M, Jones SA, Topley N, Kotecha S & O’Donnell VB. (2011) Esterified Eicosanoids are Acutely Generated by 5-Lipoxygenase in Primary Human Neutrophils and in Human and Murine Infection.  Blood 117(6), 2033-43

Kerfoot SM, McRae K, Lam F, McAvoy EF, Clark SR, Brain M, Lalor PF, Adams DH & Kubes P. (2008) A Novel Mechanism of Erythrocyte Capture from Circulation in Humans. Experimental Hematology 36(2), 111-8

Clark SR, Ma AC, Tavener AS, McDonald B, Goodarzi Z, Kelly MM, Patel KD, Chakrabarti S, McAvoy E, Sinclair GD, Keys EM, Allen-Vercoe E, DeVinney R, Doig CJ, Green FHY & Kubes P.  (2007) Platelet Toll-Like Receptor-4 Activates Neutrophil Extracellular Traps to Ensnare Bacteria in Endotoxemic and Septic Blood.  Nature Medicine 13(4), 463-9.