Josephine Bradley

Research student, School of Biosciences

“Lipid droplets in oocytes: shedding new light on why fats are good or bad for development” I use a chemically-specific, non-invasive multiphoton microscopy technique (CARS microscopy) to observe the distribution and content of lipid droplets throughout mammalian eggs and embryos. Using this technique, I am able to investigate how the characteristics of egg or embryo lipid content might affect developmental potential, and how high fat diets or obesity may take detrimental effect.

Biography:

During my BSc Biomedical Sciences (Anatomy) at Cardiff University, I undertook a professional training year with Professor Karl Swann, investigating the roles of cell-cycle regulators in the timing of pronuclear formation following fertilisation. After completion of my BSc in 2012, I returned to Professor Swann's lab, collaborating with Professor Paola Borri, to undertake a PhD funded by the President's Research Scholarship. During my PhD, titled “Label-free Multiphoton Microscopy of Lipids in Oocytes, Eggs and Early Embryos”, I used CARS microscopy to observe and investigate the number, size, distribution and content of lipid droplets throughout mammalian egg and embryo development. I was awarded my PhD in 2016, and followed it with a brief stint as a lecturer in anatomical sciences to first year medical and dental students at Cardiff University. In April 2017 we were awarded a BBSRC grant to continue our studies into mammalian egg and embryo lipid content, so I continue my research with Professors Karl Swann and Paola Borri as a postdoc.