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Dr Rob Young 


Current Research Projects

• Structural studies of cornea after freezing with a new prototype cryoprobe.
• 3D reconstruction of keratocytes and extracellular matrix in developing avian cornea by serial block face scanning electron microscopy to investigate corneal stromal development.
• Comparative ultrastructural investigation of the trabecular meshwork in normal and glaucomatous human eyes.
• Microscopical studies of corneal endothelial cells in Fuchs corneal endothelial dystrophy.

Legends for Figures

Regular arrangement of collagen fibrils in adjacent lamellae of the corneal stroma, viewed in ultrathin section by transmission electron microscopy. Bar, 1µm.

Figure 1: Regular arrangement of collagen fibrils in adjacent lamellae of the corneal stroma, viewed in ultrathin section by transmission electron microscopy. Bar, 1µm.

Stereo pair of tilted images from electron tomography of human sclera showing collagen fibrils (blue) and associated proteoglycans (orange). Bar, 200nm.

Figure 2: Stereo pair of tilted images from electron tomography of human sclera showing collagen fibrils (blue) and associated proteoglycans (orange). Bar, 200nm.

Collagen fibrils in corneal stroma viewed by transmission electron microscopy, are shown here running left to right in longitudinal section, and are associated with irregular branching proteoglycan molecules. Bar, 300nm.

Figure 3: Collagen fibrils in corneal stroma viewed by transmission electron microscopy, are shown here running left to right in longitudinal section, and are associated with irregular branching proteoglycan molecules. Bar, 300nm.

3D reconstructions from serial block face scanning electron microscopy of embryonic cornea: A. raw dataset showing collagen fibril bundles (cb) and keratopodial processes (arrows) of keratocytes (k). B. 3D reconstruction reveals close association between keratopodia (pink), and collagen fibril bundles (blue). C. Orthogonal orientation of keratopodia aligns with arrangement of collagen bundles (D). Bars. 500 nm, A; 1 µm, B,C,D.

Figure 4: 3D reconstructions from serial block face scanning electron microscopy of embryonic cornea: A. raw dataset showing collagen fibril bundles (cb) and keratopodial processes (arrows) of keratocytes (k). B. 3D reconstruction reveals close association between keratopodia (pink), and collagen fibril bundles (blue). C. Orthogonal orientation of keratopodia aligns with arrangement of collagen bundles (D). Bars. 500 nm, A; 1 µm, B,C,D.

 

Funding:

Quantock AJ (PI), K Meek & C Tucker: £836,976. A physical characterisation of assembly mechanisms and light transmission in the cornea.  EPSRC project grant. 2008 – 2011

Research Collaborators:

Professor Shigeru Kinoshita, Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
Professor Noriko Koizumi, Centre for Regenerative Medicine, Department of Biomedical Engineering, Doshisha University, Kyoto, Japan:  A New Minimally Invasive Surgery for the Treatment of Corneal Endothelial Disease