Dr Philip Lewis
Telephone:+44 (0)29 208 70459
• To understand and model corneal transparency at the cellular and fibrillar level taking advantage of new developments in three dimensional ultrastuctural imaging by Serial Block Face Field Emission Scanning Electron Microscopy
• The development of biological artificial corneal replacements.
• Understanding the structural basis for loss of transparency or refractive function in the pathological cornea.
• Corneal development.
• Three dimensional structure of the proteoglycans in the cornea and how it controls corneal transparency.
I currently manage the Structural Biophysics groups Zeiss Sigma VP Field Emission Scanning Electron Microscope with 3view 2 (Gatan) and Jeol 1010 100Kv Transmission Electron Microscope. As an experienced electron microscopist I also provide training in electron microscopy, EM sample preparation and ultramicrotomy to students within the Biophysics group.
Structural interactions between collagen and proteoglycans are elucidated by three-dimensional electron tomography of bovine cornea.
Lewis P, Pinali C, Young RD, Quantock AJ, Meek KM, Knupp C.
Structure 18, 1-7, February 10, 2010
Structural Basis for Corneal Transparency
Knupp C. Pinali C, Lewis P, Young RD, Parfitt G, Quantock AJ, Meek KM,
Advances in Protein Chemistry and Structural Biology Volume 87, Dec 2009
Three-dimensional reconstruction of collagen-proteoglycan
interactions in the mouse corneal stroma.
Parfitt GJ, Pinali C, Lewis PN, Young RD, Quantock AJ, Knupp C.
Journal of Structural Biology. 170(2):392-7. (2010)
Three Dimensional Reconstruction of the Cornea by Electron Tomography.
Geraint Parfitt, Pinali C, Philip Lewis, Young RD, Quantock AJ, Knupp C.
Microscopy and Analysis 24(2), 17-19.
Matrix morphogenesis in cornea is mediated by the modification of keratan sulfate by GlcNAc 6-O-sulfotransferase.
Hayashida Y, Akama TO, Beecher N, Lewis P, Young RD, Meek KM, Kerr B, Hughes CE, Caterson B, Tanigami A, Nakayama J, Fukada MN, Tano Y, Nishida K, Quantock AJ.
Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13333-8. Epub 2006 Aug 25.
Sulphated Proteoglycans in the Cornea Stroma of Chst5-Null Mice
Philip N Lewis,1 Yasutaka Hayashida,2 Robert D. Young,1 Tomoya O. Akama,3 Michiko N. Fukada,3 Bruce Caterson,4 Kohji Nishida,5 & Andrew J. Quantock.1
Invest Ophthalmol Vis Sci 2006;47: E-Abstract 2998.
Matrix Alterations in the Corneas of Chst5 Null Mice
A.J. Quantock1,A, Y. Hayashida2, N. Beecher1,A, P.N. Lewis 1,A, B. Caterson1,B, A. Tanigami3, T.O. Akama4, M.N. Fukada4, Y. Tano2 and K. Nishida2
Invest Ophthalmol Vis Sci 2005;46: E-Abstract 3560.