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Yr Athro Les Baillie
Athro Microbioleg
Ysgol Fferylliaeth a Gwyddorau Fferyllol
- Ar gael fel goruchwyliwr ôl-raddedig
Trosolwyg
Cymwysterau
Graddiais yn Faglor mewn Gwyddoniaeth o Brifysgol Plymouth ym 1982 ac ennill MPhil (yn rhan-amser) o Brifysgol Gorllewin Lloegr ym 1991 a doethuriaeth (rhan-amser) o Brifysgol Sheffield yn 2001.
Prosiectau pwysig
Rwy’n rhan o brosiect biosynwyryddion rhyngwladol sy’n cael ei gyllido gan NATO (2021) a’i arwain gan Brifysgol Caerdydd o'r enw ‘Synhwyrydd amser real newydd sy’n seiliedig ar nanoronynnau ar gyfer B. anthracis a M. twbercwlosis’. Mae’r tîm yn cynnwys sefydliadau yn yr Wcráin (Sefydliad Geocemeg Amgylcheddol y Wladwriaeth, Sefydliad Cemeg Arwyneb Chuiko a Chanolfan Glinigol a Phroffylactig "Phthisioleg" Cyngor Rhanbarthol Dnipropetrivsk) a'r Eidal (Istituto Zooprofilattico Sperimenttale dell Puglia e dell Basillicata).
Mae Trio Sci Cymru (2019) sy’n cael ei arwain gan Academi Wyddoniaeth Genedlaethol Llywodraeth Cymru yn brosiect lle rydym yn gweithio gyda 3,172 o ddisgyblion cyfnod allweddol 3 i hybu’r nifer sy’n astudio pynciau STEM. Mae is-thema gwenyn Apothecari yn cyflwyno disgyblion i bwysigrwydd gwenyn a pheillwyr eraill, priodweddau meddyginiaethol mêl a’i botensial i drin archfygiau sy’n gallu gwrthsefyll gwrthfiotigau mewn ysbytai.
Mae prosiect Pharmabees yn rhan o Genhadaeth Sifig y Brifysgol i gefnogi iechyd a lles pobl Cymru. Mae’r fenter arobryn hon yn cefnogi gweithgareddau yn y gymuned ac yn yr ysgolion sy’n canolbwyntio ar bwysigrwydd peillwyr a bioamrywiaeth. Mae hefyd yn cydweithio â diwydiant Cymru i greu cynhyrchion arloesol yn seiliedig ar adnoddau naturiol sy'n hybu iechyd.
Newyddion
- Te lles gyda mêl - Ionawr 2021
- Bertie Bumbles search for Magic Medicine - Gorffennaf 2020
- Prosiect hadau blodau gwyllt - Mehefin 2020
- Prosiect gwyddoniaeth dinasyddion Spot-a-bee - Mai 2020
- Cwrw mêl - Rhagfyr 2017
- 12fed Cyngres Microbioleg Milfeddygol, Twrci - Medi 2016
- Oes gan wenyn acenion? - Gorffennaf 2016
- Fferylliaeth yn cael ei chynrychioli yn 15fed Cynhadledd Bioamddiffyn Meddygol - Mai 2016
- Siaradwr gwadd ym 44ain Cynhadledd y Sefydliad Meddygaeth Ataliol - Mawrth 2016
- Ymchwil gyhoeddedig ymysg y 10 uchaf o ran lawrlwythiadau ym mis Chwefror 2016
- Cyfweliad gydag 'Inside Science' Radio 4 am y Pharma Bees - Hydref 2015
- Pharmabees Hydref 2015
- Gweithdy Anthrax gan Nato a chymorth yr UE yn Georgia Medi 2015
- Gwenyn yn brwydro yn erbyn archfyg, fis Awst 2015
- Diwrnod yr amgylchedd Hydref 2014
- Gwenyn mêl a diwrnod hyfforddiant athrawon Gorffennaf 2014
Gwefannau perthnasol
Cyhoeddiad
2024
- Wilson-Garner, S., Alzeer, S., Baillie, L. and Porch, A. 2024. High-volume biological sample processing using microwaves. Journal of Applied Physics 135(4), article number: 44901. (10.1063/5.0178755)
- Buyuk, F., Dyson, H., Laws, T. R., Celebi, O., Doganay, M., Sahin, M. and Baillie, L. 2024. Human exposure to naturally occurring Bacillus anthracis in the Kars region of Eastern Türkiye. Microorganisms 12(1), article number: 167. (10.3390/microorganisms12010167)
2023
- Ahortor, E., Baillie, L., Lloyd, D. and Blaxland, J. 2023. Virucidal efficacy of gaseous ozone against type 1 herpes simplex virus (hsv-1). Ozone: Science & Engineering (10.1080/01919512.2023.2231037)
- Alyahya, K. and Baillie, L. 2023. Assessing the feasibility of employing a combination of a bacteriophage-derived endolysin and spore germinants to treat relapsing clostridioides difficile infection. Microorganisms 11(7), article number: 1651. (10.3390/microorganisms11071651)
- Taylor-Joyce, G. et al. 2023. The influence of extrachromosomal elements in the anthrax “cross-over” strain Bacillus cereus G9241. Frontiers in Microbiology 14, article number: 1113642. (10.3389/fmicb.2023.1113642)
- Doganay, M., Dinc, G., Kutmanova, A. and Baillie, L. 2023. Human anthrax: Update of the diagnosis and treatment. Diagnostics 13(6), article number: 1056. (10.3390/diagnostics13061056)
- Manoharan, S. et al. 2023. From cereus to anthrax and back again: Assessment of the temperature-dependent phenotypic switching in the "cross-over" strain Bacillus cereus G9241. Frontiers in Microbiology 14 (10.3389/fmicb.2023.1113562)
- Wilson-Garner, S., Baillie, L. and Porch, A. 2023. Increasing sample volume for microwave-assisted rapid DNA release. Presented at: 2022 Asia-Pacific Microwave Conference (APMC), Yokohama, Japan, 29 November 2022 - 02 December 2022Proceedings of 2022 Asia-Pacific Microwave Conference (APMC). IEEE pp. 638-640., (10.23919/APMC55665.2022.10000050)
2022
- Ascough, S. et al. 2022. Impact of HLA polymorphism on the immune response to Bacillus anthracis protective antigen in vaccination versus natural infection. Vaccines 10(10), article number: 1571. (10.3390/vaccines10101571)
- Blaxland, J., Thomas, R. and Baillie, L. 2022. The antibacterial effect of Humulus lupulus (Hops) against Mycobacterium bovis BCG: a promising alternative in the fight against bovine tuberculosis?. Beverages 8(3), article number: e43. (10.3390/beverages8030043)
2021
- Blaxland, J. A., Watkins, A. J. and Baillie, L. W. J. 2021. The ability of hop extracts to reduce the methane production of methanobrevibacter ruminantium. Archaea 2021, article number: 5510063. (10.1155/2021/5510063)
- Goggin, K. and Baillie, L. 2021. Can bees help us find new antibiotics?. Frontiers for Young Minds 9, article number: 611604. (10.3389/frym.2021.611604)
- Blaxland, J., Thomas, R. and Baillie, L. 2021. Development of the School Science Club at Cardiff University. Research for All 5(1), pp. 86–100. (10.14324/RFA.05.1.08)
2020
- Ahortor, E. K., Malyshev, D., Williams, C. F., Choi, H., Lees, J., Porch, A. and Baillie, L. 2020. The biological effect of 2.45 GHz microwaves on the viability and permeability of bacterial and yeast cells. Journal of Applied Physics 127(204902), pp. IMPORTED. (10.1063/1.5145009)
- Malyshev, D. and Baillie, L. 2020. Surface morphology differences in Clostridium difficile spores, based on different strains and methods of purification. Anaerobe 61, article number: 102078. (10.1016/j.anaerobe.2019.102078)
2019
- Gallagher, T. B., Mellado-Sanchez, G., Jorgensen, A. L., Moore, S., Nataro, J. P., Pasetti, M. F. and Baillie, L. W. 2019. Development of a multiple-antigen protein fusion vaccine candidate that confers protection against Bacillus anthracis and Yersinia pestis. PLoS Neglected Tropical Diseases 13(8), article number: e0007644. (10.1371/journal.pntd.0007644)
- Hamzah, H., Ahortor, E., Malyshev, D., Choi, H., Lees, J., Baillie, L. and Porch, A. 2019. A compact microwave applicator for the rapid detection of clostridium difficile. Presented at: 2019 IEEE MTT-S International Microwave Biomedical Conference (IMBioC), Nanjing, China, 6-8 May 20192019 IEEE MTT-S International Microwave Biomedical Conference (IMBioC). IEEE, (10.1109/IMBIOC.2019.8777882)
- Malyshev, D., Williams, C. F., Lees, J., Baillie, L. and Porch, A. 2019. Model of microwave effects on bacterial spores. Journal of Applied Physics 125(12), article number: 124701. (10.1063/1.5085442)
2018
- Sahin, M. et al. 2018. The identification of novel single nucleotide polymorphisms to assist in mapping the spread of Bacillus anthracis across the Southern Caucasus. Scientific Reports 8(1), article number: 11254. (10.1038/s41598-018-29738-3)
- Moore, A., Davies, T., Berube, K., Jones, T. and Baillie, L. 2018. Bio-reactive clay minerals and anthrax decontamination: a novel antimicrobial solution. Presented at: Focused Meeting on Emerging Zoonoses and Antimicrobial Resistance, School of Veterinary Medicine, University of Surrey, Guildford, UK, 2 July 2018.
- Ingavle, G., Baillie, L., Davies, N., Beaton, N., Zheng, Y., Mikhalovsky, S. and Sandeman, S. 2018. Bioinspired detoxification of blood: The efficient removal of anthrax toxin protective antigen using an extracorporeal macroporous adsorbent device. Scientific Reports 8(1), pp. -., article number: 7518. (10.1038/s41598-018-25678-0)
- Baptista, R., Bhowmick, S., Nash, R. J., Baillie, L. and Mur, L. A. 2018. Target discovery focused approaches to overcome bottlenecks in the exploitation of antimycobacterial natural products. Future Medicinal Chemistry 10(7), pp. 811-822. (10.4155/fmc-2017-0273)
- Baptista, R., Fazakerley, D. M., Beckmann, M., Baillie, L. and Mur, L. A. J. 2018. Untargeted metabolomics reveals a new mode of action of pretomanid (PA-824). Scientific Reports 8(1), article number: 5084. (10.1038/s41598-018-23110-1)
- Schelkle, B. et al. 2018. Caenorhabditis elegans predation on Bacillus anthracis: Decontamination of spore contaminated soil with germinants and nematodes. Frontiers in Microbiology 8, article number: 2601. (10.3389/fmicb.2017.02601)
- Centeleghe, I., Myles, E., Baillie, L., Berube, K., Jones, T. and Blaxland, J. 2018. Combating the rising global threat of antimicrobial resistance with clay minerals: A study on two major hospital superbugs. Presented at: Focused meeting 2018: Emerging zoonoses and AMR: A global threat, University of Surrey, 02-02 July 2018.
2017
- Khmaladze, E. et al. 2017. Molecular genotyping of bacillus anthracis strains from Georgia and northeastern part of Turkey. Journal of Bacteriology and Mycology 4(3)
- Joshi, L. T., Welsch, A., Hawkins, J. and Baillie, L. 2017. The effect of hospital biocide sodium dichloroisocyanurate on the viability and properties of Clostridium difficile spores. Letters in Applied Microbiology 65(3), pp. 199-205. (10.1111/lam.12768)
2016
- Cooper, C. et al. 2016. Virulence plasmid stability in environmentally occurring Bacillus anthracis from North East Turkey. Antonie van Leeuwenhoek 110(1), pp. 167-170. (10.1007/s10482-016-0767-5)
- Dyer, P. D. et al. 2016. An in vitro evaluation of epigallocatechin gallate (eGCG) as a biocompatible inhibitor of ricin toxin. Biochimica et Biophysica Acta (BBA) - General Subjects 1860(7), pp. 1541-1550. (10.1016/j.bbagen.2016.03.024)
- Celebi, O. et al. 2016. The use of germinants to potentiate the sensitivity of Bacillus anthracis spores to peracetic acid. Frontiers in Microbiology 7, article number: 18. (10.3389/fmicb.2016.00018)
- Ascough, S. et al. 2016. CD4+ T cells targeting dominant and cryptic epitopes from Bacillus anthracis lethal factor. Frontiers in Microbiology 6, article number: 1506. (10.3389/fmicb.2015.01506)
- Eissa, A. G. et al. 2016. Targeting methionyl tRNA synthetase: design, synthesis and antibacterial activity against Clostridium difficileof novel 3-biaryl-N-benzylpropan-1-amine derivatives. Journal of Enzyme Inhibition and Medicinal Chemistry 31(6), pp. 1694-1697. (10.3109/14756366.2016.1140754)
2015
- Dyer, P. D. et al. 2015. Disarmed anthrax toxin delivers antisense oligonucleotides and siRNA with high efficiency and low toxicity. Journal of Controlled Release 220(A), pp. 316-328. (10.1016/j.jconrel.2015.10.054)
- Hawkins, J. et al. 2015. Using DNA metabarcoding to identify the floral composition of honey: A new tool for investigating honey bee foraging preferences. PLoS ONE 10(8), article number: e0134735. (10.1371/journal.pone.0134735)
- Buyuk, F. et al. 2015. The effect of prolonged storage on the virulence of isolates of Bacillus anthracis obtained from environmental and animal sources in the Kars Region of Turkey. FEMS Microbiology Letters 362(13), article number: fnv102. (10.1093/femsle/fnv102)
- Köhler, S. M., Baillie, L. and Beyer, W. 2015. BclA and toxin antigens augment each other to protect NMRI mice from lethal Bacillus anthracis challenge. Vaccine 33(24), pp. 2771-2777. (10.1016/j.vaccine.2015.04.049)
- Ingavle, G. C. et al. 2015. Affinity binding of antibodies to supermacroporous cryogel adsorbents with immobilized protein A for removal of anthrax toxin protective antigen. Biomaterials 50, pp. 140-153. (10.1016/j.biomaterials.2015.01.039)
- Ingram, R. J. et al. 2015. Natural cutaneous anthrax infection, but not vaccination, induces a CD4+ T cell response involving diverse cytokines. Cell & Bioscience 5, article number: 20. (10.1186/s13578-015-0011-4)
- Stedmon, A. W., Eachus, P., Baillie, L., Tallis, H., Donkor, R., Edlin-White, R. and Bracewell, R. 2015. Scalable interrogation: Eliciting human pheromone responses to deception in a security interview setting. Applied Ergonomics 47, pp. 26-33. (10.1016/j.apergo.2014.08.015)
2014
- Joshi, L. T., Mali, B. L., Geddes, C. D. and Baillie, L. 2014. Extraction and sensitive detection of toxins A and B from the human pathogen clostridium difficile in 40 seconds using microwave-accelerated metal-enhanced fluorescence. PLoS ONE 9(8), article number: e104334. (10.1371/journal.pone.0104334)
- Ascough, S. et al. 2014. Anthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T Cell immunity. PLoS Pathogens 10(5), article number: e1004085. (10.1371/journal.ppat.1004085)
2013
- Gallagher, T. and Baillie, L. 2013. Anthrax in zoonoses. In: Palmer, S. R. et al. eds. Oxford Textbook of Zoonoses Biology, Clinical Practice, and Public Health Control. Second Edition. Oxford Textbooks In Public Health Oxford University Press
- Eachus, P., Stedmon, A. and Baillie, L. 2013. Hostile intent in public crowded spaces: A field study. Applied Ergonomics 44(5), pp. 703-709. (10.1016/j.apergo.2012.05.009)
- Ingram, R. J. et al. 2013. Exposure to anthrax toxin alters human leukocyte expression of Anthrax toxin receptor 1. Clinical and Experimental Immunology 173(1), pp. 84-91. (10.1111/cei.12090)
- Baillie, L. 2013. Can one size fit all? Towards a universal anthrax vaccine [Editorial]. Future Microbiology 8(3), pp. 295-297. (10.2217/fmb.13.11)
- Williams, G. J., Linley, E., Nicholas, R. and Baillie, L. 2013. The role of the exosporium in the environmental distribution of anthrax. Journal of Applied Microbiology 114(2), pp. 396-403. (10.1111/jam.12034)
- Zhang, J. et al. 2013. An adenovirus-vectored nasal vaccine confers rapid and sustained protection against Anthrax in a single-dose regimen. Clinical and Vaccine Immunology 20(1), pp. 1-8. (10.1128/CVI.00280-12)
- Baillie, L. and Theriault, S. 2013. The control of biological agents. In: Fraise, A. P., Maillard, J. and Satar, S. eds. Principles and Practice of Disinfection, Preservation and Sterilization. Wiley-Blackwell, pp. 576.
2012
- Joshi, L. T., Phillips, D. S., Williams, C. F., Alyousef, A. and Baillie, L. 2012. Contribution of Spores to the Ability of Clostridium difficile To Adhere to Surfaces. Applied and Environmental Microbiology 78(21), pp. 7671-7679. (10.1128/AEM.01862-12)
- Ascough, S. et al. 2012. Identification of immunodominant T cell epitopes from anthrax protective antigen for inclusion in a rationally designed sub-unit based vaccine [Abstract]. Immunology 137(S1), pp. 761. (10.1111/imm.12002)
- Albrecht, M. T., Eyles, J. E., Baillie, L. and Keane-Myers, A. M. 2012. Immunogenicity and efficacy of an anthrax/plague DNA fusion vaccine in a mouse model. FEMS Immunology & Medical Microbiology 65(3), pp. 505-509. (10.1111/j.1574-695X.2012.00974.x)
- Ingram, R. and Baillie, L. 2012. It's in the genes! Human genetic diversity and the response to anthrax vaccines [Editorial]. Expert Review of Vaccines 11(6), pp. 633-635. (10.1586/erv.12.41)
- Baillie, L. and Theriault, S. 2012. Control of infectious bioagents. In: Fraise, A., Maillard, J. and Sattar, S. eds. Russell, Hugo & Ayliffe's Principles and Practice of Disinfection, Preservation & Sterilization. Oxford: Wiley-Blackwell, pp. 576-588., (10.1002/9781118425831.ch23)
2011
- Brenneman, K. E. et al. 2011. The early humoral immune response to Bacillus anthracis toxins in patients infected with cutaneous anthrax. FEMS Immunology & Medical Microbiology 62(2), pp. 164-172. (10.1111/j.1574-695X.2011.00800.x)
- Mett, V. et al. 2011. A non-glycosylated, plant-produced human monoclonal antibody against anthrax protective antigen protects mice and non-human primates from B. anthracis spore challenge. Human Vaccines 7, pp. 183-190. (10.4161/hv.7.0.14586)
- Baillie, L., Dyson, H. and Simpson, A. 2011. Dual use of biotechnology. In: Singer, P., Callahan, D. and Chadwick, R. F. eds. Encyclopedia of Applied Ethics. London: Academic Press Inc
2010
- Porasuphatana, S. et al. 2010. Bacillus Anthracis Endospores Regulate Ornithine Decarboxylase and Inducible Nitric Oxide Synthase Through ERK1/2 and p38 Mitogen-Activated Protein Kinases. Current Microbiology 61(6), pp. 567-573. (10.1007/s00284-010-9654-x)
- Baillie, L. et al. 2010. An anthrax subunit vaccine candidate based on protective regions of Bacillus anthracis protective antigen and lethal factor. Vaccine 28(41), pp. 6740-6748. (10.1016/j.vaccine.2010.07.075)
- Ramirez, K., Ditamo, Y., Galen, J. E., Baillie, L. and Pasetti, M. F. 2010. Mucosal priming of newborn mice with S. Typhi Ty21a expressing anthrax protective antigen (PA) followed by parenteral PA-boost induces B and T cell-mediated immunity that protects against infection bypassing maternal antibodies. Vaccine 28(37), pp. 6065-6075. (10.1016/j.vaccine.2010.06.089)
- Ingram, R. J. et al. 2010. An Epitope of Bacillus anthracis Protective Antigen That Is Cryptic in Rabbits May Be Immunodominant in Humans [Letter]. Infection and Immunity 78(5), pp. 2353-2354. (10.1128/IAI.00072-10)
- Ingram, R. J. et al. 2010. Natural exposure to cutaneous anthrax gives long-lasting T cell immunity encompassing infection-specific epitopes. Journal of Immunology 184(7), pp. 3814-3821. (10.4049/jimmunol.0901581)
2009
- Vernazza, C., Lingard, B., Flick-Smith, H. C., Baillie, L., Hill, J. and Atkins, H. S. 2009. Small protective fragments of the Yersinia pestis V antigen. Vaccine 27(21), pp. 2775-2780. (10.1016/j.vaccine.2009.03.011)
2008
- Baillie, L., Rodriguez, A. L., Moore, S., Atkins, H. S., Feng, C., Nataro, J. P. and Pasetti, M. F. 2008. Towards a human oral vaccine for anthrax: the utility of a Salmonella Typhi Ty21a-based prime-boost immunization strategy. Vaccine 26(48), pp. 6083-6091. (10.1016/j.vaccine.2008.09.010)
- Aslan, K., Previte, M. J. R., Zhang, Y. X., Gallagher, T., Baillie, L. and Geddes, C. D. 2008. Extraction and detection of DNA from Bacillus anthracis spores and the vegetative cells within 1 min. Analytical Chemistry (including News & Features) 80(11), pp. 4125-4132. (10.1021/ac800519r)
- Kang, T. J., Basu, S., Zhang, L., Thomas, K. E., Vogel, S. N., Baillie, L. and Cross, A. S. 2008. Bacillus anthracis spores and lethal toxin induce IL-1 beta via functionally distinct signaling pathways. European Journal of Immunology 38(6), pp. 1574-1584. (10.1002/eji.200838141)
2007
- Basu, S., Kang, T. J., Chen, W. H., Fenton, M. J., Baillie, L., Hibbs, S. and Cross, A. S. 2007. Role of Bacillus anthracis spore structures in macrophage cytokine responses. Infection and Immunity 75(5), pp. 2351-2358. (10.1128/IAI.01982-06)
- Stokes, M. G. M. et al. 2007. Oral administration of a Salmonella enterica-based vaccine expressing Bacillus anthracis protective antigen confers protection against aerosolized B. anthracis. Infection and Immunity 75(4), pp. 1827-1834. (10.1128/IAI.01242-06)
- Albrecht, M. T. et al. 2007. Human monoclonal antibodies against anthrax lethal factor and protective antigen act independently to protect against Bacillus anthracis infection and enhance endogenous immunity to anthrax. Infection and Immunity 75(11), pp. 5425-5433. (10.1128/iai.00261-07)
- Aslan, K., Previte, M. J. R., Zhang, Y. X., Baillie, L. and Geddes, C. D. 2007. Ultra-fast and sensitive DNA hybridization assays: Application to genomic anthrax detection in < 30 seconds. Biophysical Journal, pp. 552A-552A.
- Aslan, K., Zhang, Y. X., Hibbs, S., Baillie, L., Previte, M. J. R. and Geddes, C. D. 2007. Microwave-accelerated metal-enhanced fluorescence: application to detection of genomic and exosporium anthrax DNA in < 30 seconds. Analyst 132(11), pp. 1130-1138. (10.1039/b707876e)
- Basu, S., Kang, T. J., Chen, W. H., Fenton, M. J., Baillie, L., Hibbs, S. and Cross, A. S. 2007. Role of Bacillus anthracis spore structures in macrophage cytokine responses. Infection and Immunity 75(5), pp. 2351-2358. (10.1128/iai.01982-06)
- Hepburn, M. J. et al. 2007. Immune response to two different dosing schedules of the anthrax vaccine precipitated (AVP) vaccine. Vaccine 25(32), pp. 6089-6097. (10.1016/j.vaccine.2007.05.018)
- Legutki, J. B., Nelson, M., Titball, R., Galloway, D. R., Mateczun, A. and Baillie, L. 2007. Analysis of peptide mimotopes of Burkholderia pseudomallei exopolysaccharide. Vaccine 25(45), pp. 7796-7805. (10.1016/j.vaccine.2007.08.045)
- Stokes, M. G. M. et al. 2007. Oral administration of a Salmonella enterica-based vaccine expressing Bacillus anthracis protective antigen confers protection against aerosolized B-anthracis. Infection and Immunity 75(4), pp. 1827-1834. (10.1128/iai.01242-06)
- Weaver, J. et al. 2007. The protective role of bacillus anthracis exosporium in macrophage-mediated killing by nitric oxide. The FASEB Journal 21(5), pp. A624-A624.
- Weaver, J. et al. 2007. Protective role of Bacillus anthracis exosporium in macrophage-mediated killing by nitric oxide. Infection and Immunity 75(8), pp. 3894-3901. (10.1128/iai.00283-07)
2004
- Priest, F. G., Barker, M., Baillie, L., Holmes, E. C. and Maiden, M. C. J. 2004. Population. Journal of Bacteriology 186(23), pp. 7959-7970. (10.1128/JB.186.23.7959-7970.2004)
2003
- Baillie, L., Peterson, S., Read, T. and Tourasse, N. 2003. The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria. Nature 423(6935), pp. 81-86. (10.1038/nature01586)
- Baillie, L., Hebdon, R., Flick-Smith, H. and Williamson, D. 2003. Characterisation of the immune response to the UK human anthrax vaccine. FEMS Immunology and Medical Microbiology 36(1-2), pp. 83-86. (10.1016/S0928-8244(03)00085-3)
- Williams, R. C. et al. 2003. Production of Bacillus anthracis protective antigen is dependent on the extracellular chaperone, PrsA. Journal of Biological Chemistry 278(20), pp. 18056-18062. (10.1074/jbc.M301244200)
2002
- Williamson, E. D., Bennett, A. M., Perkins, S. D., Beedham, R. J., Miller, J. and Baillie, L. 2002. Co-immunisation with a plasmid DNA cocktail primes mice against anthrax and plague. Vaccine 20(23-24), pp. 2933-2941. (10.1016/S0264-410X(02)00232-3)
- Flick-Smith, H. C. et al. 2002. Mucosal or parenteral administration of microsphere-associated Bacillus anthracis protective antigen protects against anthrax infection in mice. Infection and Immunity 70(4), pp. 2022-2028. (10.1128/IAI.70.4.2022-2028.2002)
- Thwaite, J. E., Baillie, L., Carter, N. M., Stephenson, K., Rees, M., Harwood, C. R. and Emmerson, P. T. 2002. Optimization of the cell wall microenvironment allows increased production of recombinant Bacillus anthracis protective antigen from B-subtilis. Applied and Environmental Microbiology 68(1), pp. 227-234. (10.1128/AEM.68.1.227-234.2002)
2001
- Baillie, L. 2001. The development of new vaccines against Bacillus anthracis. Journal of Applied Microbiology 91(4), pp. 609-613. (10.1046/j.1365-2672.2001.01498.x)
- Baillie, L. and Godfree, A. 2001. Dangerous Pathogens 2000, University of Plymouth, 4-7th September 2000 [Preface]. Journal of Applied Microbiology 91(4), pp. 571. (10.1046/j.1365-2672.2001.01307.x)
2000
- Baillie, L. 2000. Bacillus anthracis. In: Batt, C. A., Robinson, R. K. and Patel, P. D. eds. The Encyclopaedia of Food Microbiology. Academic Press, pp. 129-135.
1999
- Williamson, E. D., Beedham, R. J., Bennett, A. M., Perkins, S. D., Miller, J. and Baillie, L. 1999. Presentation of protective antigen to the mouse immune system: immune sequelae. Journal of Applied Microbiology 87(2), pp. 315-317. (10.1046/j.1365-2672.1999.00901.x)
- Zegers, N. D., Kluter, E., van der Stap, H., van Dura, E., van Dalen, P., Shaw, M. and Baillie, L. 1999. Expression of the protective antigen of Bacillus anthracis by Lactobacillus casei: towards the development of an oral vaccine against anthrax. Journal of Applied Microbiology 87(2), pp. 309-314. (10.1046/j.1365-2672.1999.00900.x)
- Baillie, L., Fowler, K. and Turnbull, P. C. B. 1999. Human immune responses to the UK human anthrax vaccine. Journal of Applied Microbiology 87(2), pp. 306-308. (10.1046/j.1365-2672.1999.00899.x)
- Fowler, K., McBride, B. W., Turnbull, P. C. B. and Baillie, L. 1999. Immune correlates of protection against anthrax. Journal of Applied Microbiology 87(2), pp. 305. (10.1046/j.1365-2672.1999.00898.x)
1998
- Baillie, L., Moore, P. and McBride, B. W. 1998. A heat-inducible Bacillus subtilis bacteriophage Phi 105 expression system for the production of the protective antigen of Bacillus anthracis. FEMS Microbiology Letters 163(1), pp. 43-47. (10.1111/j.1574-6968.1998.tb13024.x)
- McBride, B., Mogg, A., Telfer, J. L., Lever, M. S., Miller, J., Turnbull, P. C. B. and Baillie, L. 1998. Protective efficacy of a recombinant protective antigen against Bacillus anthracis challenge and assessment of immunological markers. Vaccine 16(8), pp. 810-817. (10.1016/S0264-410X(97)00268-5)
- Baillie, L., Moir, A. and Manchee, R. 1998. The expression of the protective antigen of Bacillus anthracis in Bacillus subtilis. Journal of Applied Microbiology 84(5), pp. 741-746. (10.1046/j.1365-2672.1998.00405.x)
- Miller, J., McBride, B. W., Manchee, R. J., Moore, P. and Baillie, L. 1998. Production and purification of recombinant protective antigen and protective efficacy against Bacillus anthracis. Letters in Applied Microbiology 26(1), pp. 56-60. (10.1046/j.1472-765X.1998.00274.x)
1995
- Baillie, L., Jones, M. N., Turnbull, P. C. B. and Manchee, R. J. 1995. Evaluation of the Biolog system for the identification of Bacillus anthracis. Letters in Applied Microbiology 20(4), pp. 209-211. (10.1111/j.1472-765X.1995.tb00429.x)
1994
- Baillie, L., Johnson, M. and Manchee, R. J. 1994. Evaluation of Bacillus subtilis strain IS53 for the production of Bacillus anthracis protective antigen. Letters in Applied Microbiology 19(4), pp. 225-227. (10.1111/j.1472-765X.1994.tb00949.x)
1993
- Baillie, L., Wade, J. J. and Casewell, M. W. 1993. Colonial variation in vancomycin resistant Enterococcus faecium. Journal of Clinical Pathology 46(5), pp. 474-475. (10.1136/jcp.46.5.474)
1992
- Wade, J., Baillie, L., Rolando, N. and Casewell, M. 1992. Pristinamycin for Enterococcus faecium resistant to vancomycin and gentamicin. Lancet 339(8788), pp. 312-313. (10.1016/0140-6736(92)91391-K)
1990
- Baillie, L. 1990. Rapid identification of Klebsiella [Letter]. Journal of Clinical Pathology 43(10), pp. 875. (10.1136/jcp.43.10.875-b)
1989
- Baillie, L. 1989. Ciproflaxacin-resistant pseudomonas-aeruginosa strain. Medical Laboratory Sciences 46(1), pp. 92-93.
1987
- Baillie, L. 1987. A survey of the incidence of penicillin-resistant beta-lactamase-negative strains of staphylococcus-aureus. Medical Laboratory Sciences 44(3), pp. 285-286.
- Baillie, L. 1987. Chlorhexidine resistance among bacteria isolated from urine of catheterized patients [Short report]. Journal of Hospital Infection 10(1), pp. 83-86. (10.1016/0195-6701(87)90037-5)
1986
- Baillie, L. 1986. Routine screening of catheter urine specimens for chlorhexidine resistant organisms. Medical Laboratory Sciences 43(3), pp. 284-285.
Articles
- Wilson-Garner, S., Alzeer, S., Baillie, L. and Porch, A. 2024. High-volume biological sample processing using microwaves. Journal of Applied Physics 135(4), article number: 44901. (10.1063/5.0178755)
- Buyuk, F., Dyson, H., Laws, T. R., Celebi, O., Doganay, M., Sahin, M. and Baillie, L. 2024. Human exposure to naturally occurring Bacillus anthracis in the Kars region of Eastern Türkiye. Microorganisms 12(1), article number: 167. (10.3390/microorganisms12010167)
- Ahortor, E., Baillie, L., Lloyd, D. and Blaxland, J. 2023. Virucidal efficacy of gaseous ozone against type 1 herpes simplex virus (hsv-1). Ozone: Science & Engineering (10.1080/01919512.2023.2231037)
- Alyahya, K. and Baillie, L. 2023. Assessing the feasibility of employing a combination of a bacteriophage-derived endolysin and spore germinants to treat relapsing clostridioides difficile infection. Microorganisms 11(7), article number: 1651. (10.3390/microorganisms11071651)
- Taylor-Joyce, G. et al. 2023. The influence of extrachromosomal elements in the anthrax “cross-over” strain Bacillus cereus G9241. Frontiers in Microbiology 14, article number: 1113642. (10.3389/fmicb.2023.1113642)
- Doganay, M., Dinc, G., Kutmanova, A. and Baillie, L. 2023. Human anthrax: Update of the diagnosis and treatment. Diagnostics 13(6), article number: 1056. (10.3390/diagnostics13061056)
- Manoharan, S. et al. 2023. From cereus to anthrax and back again: Assessment of the temperature-dependent phenotypic switching in the "cross-over" strain Bacillus cereus G9241. Frontiers in Microbiology 14 (10.3389/fmicb.2023.1113562)
- Ascough, S. et al. 2022. Impact of HLA polymorphism on the immune response to Bacillus anthracis protective antigen in vaccination versus natural infection. Vaccines 10(10), article number: 1571. (10.3390/vaccines10101571)
- Blaxland, J., Thomas, R. and Baillie, L. 2022. The antibacterial effect of Humulus lupulus (Hops) against Mycobacterium bovis BCG: a promising alternative in the fight against bovine tuberculosis?. Beverages 8(3), article number: e43. (10.3390/beverages8030043)
- Blaxland, J. A., Watkins, A. J. and Baillie, L. W. J. 2021. The ability of hop extracts to reduce the methane production of methanobrevibacter ruminantium. Archaea 2021, article number: 5510063. (10.1155/2021/5510063)
- Goggin, K. and Baillie, L. 2021. Can bees help us find new antibiotics?. Frontiers for Young Minds 9, article number: 611604. (10.3389/frym.2021.611604)
- Blaxland, J., Thomas, R. and Baillie, L. 2021. Development of the School Science Club at Cardiff University. Research for All 5(1), pp. 86–100. (10.14324/RFA.05.1.08)
- Ahortor, E. K., Malyshev, D., Williams, C. F., Choi, H., Lees, J., Porch, A. and Baillie, L. 2020. The biological effect of 2.45 GHz microwaves on the viability and permeability of bacterial and yeast cells. Journal of Applied Physics 127(204902), pp. IMPORTED. (10.1063/1.5145009)
- Malyshev, D. and Baillie, L. 2020. Surface morphology differences in Clostridium difficile spores, based on different strains and methods of purification. Anaerobe 61, article number: 102078. (10.1016/j.anaerobe.2019.102078)
- Gallagher, T. B., Mellado-Sanchez, G., Jorgensen, A. L., Moore, S., Nataro, J. P., Pasetti, M. F. and Baillie, L. W. 2019. Development of a multiple-antigen protein fusion vaccine candidate that confers protection against Bacillus anthracis and Yersinia pestis. PLoS Neglected Tropical Diseases 13(8), article number: e0007644. (10.1371/journal.pntd.0007644)
- Malyshev, D., Williams, C. F., Lees, J., Baillie, L. and Porch, A. 2019. Model of microwave effects on bacterial spores. Journal of Applied Physics 125(12), article number: 124701. (10.1063/1.5085442)
- Sahin, M. et al. 2018. The identification of novel single nucleotide polymorphisms to assist in mapping the spread of Bacillus anthracis across the Southern Caucasus. Scientific Reports 8(1), article number: 11254. (10.1038/s41598-018-29738-3)
- Ingavle, G., Baillie, L., Davies, N., Beaton, N., Zheng, Y., Mikhalovsky, S. and Sandeman, S. 2018. Bioinspired detoxification of blood: The efficient removal of anthrax toxin protective antigen using an extracorporeal macroporous adsorbent device. Scientific Reports 8(1), pp. -., article number: 7518. (10.1038/s41598-018-25678-0)
- Baptista, R., Bhowmick, S., Nash, R. J., Baillie, L. and Mur, L. A. 2018. Target discovery focused approaches to overcome bottlenecks in the exploitation of antimycobacterial natural products. Future Medicinal Chemistry 10(7), pp. 811-822. (10.4155/fmc-2017-0273)
- Baptista, R., Fazakerley, D. M., Beckmann, M., Baillie, L. and Mur, L. A. J. 2018. Untargeted metabolomics reveals a new mode of action of pretomanid (PA-824). Scientific Reports 8(1), article number: 5084. (10.1038/s41598-018-23110-1)
- Schelkle, B. et al. 2018. Caenorhabditis elegans predation on Bacillus anthracis: Decontamination of spore contaminated soil with germinants and nematodes. Frontiers in Microbiology 8, article number: 2601. (10.3389/fmicb.2017.02601)
- Khmaladze, E. et al. 2017. Molecular genotyping of bacillus anthracis strains from Georgia and northeastern part of Turkey. Journal of Bacteriology and Mycology 4(3)
- Joshi, L. T., Welsch, A., Hawkins, J. and Baillie, L. 2017. The effect of hospital biocide sodium dichloroisocyanurate on the viability and properties of Clostridium difficile spores. Letters in Applied Microbiology 65(3), pp. 199-205. (10.1111/lam.12768)
- Cooper, C. et al. 2016. Virulence plasmid stability in environmentally occurring Bacillus anthracis from North East Turkey. Antonie van Leeuwenhoek 110(1), pp. 167-170. (10.1007/s10482-016-0767-5)
- Dyer, P. D. et al. 2016. An in vitro evaluation of epigallocatechin gallate (eGCG) as a biocompatible inhibitor of ricin toxin. Biochimica et Biophysica Acta (BBA) - General Subjects 1860(7), pp. 1541-1550. (10.1016/j.bbagen.2016.03.024)
- Celebi, O. et al. 2016. The use of germinants to potentiate the sensitivity of Bacillus anthracis spores to peracetic acid. Frontiers in Microbiology 7, article number: 18. (10.3389/fmicb.2016.00018)
- Ascough, S. et al. 2016. CD4+ T cells targeting dominant and cryptic epitopes from Bacillus anthracis lethal factor. Frontiers in Microbiology 6, article number: 1506. (10.3389/fmicb.2015.01506)
- Eissa, A. G. et al. 2016. Targeting methionyl tRNA synthetase: design, synthesis and antibacterial activity against Clostridium difficileof novel 3-biaryl-N-benzylpropan-1-amine derivatives. Journal of Enzyme Inhibition and Medicinal Chemistry 31(6), pp. 1694-1697. (10.3109/14756366.2016.1140754)
- Dyer, P. D. et al. 2015. Disarmed anthrax toxin delivers antisense oligonucleotides and siRNA with high efficiency and low toxicity. Journal of Controlled Release 220(A), pp. 316-328. (10.1016/j.jconrel.2015.10.054)
- Hawkins, J. et al. 2015. Using DNA metabarcoding to identify the floral composition of honey: A new tool for investigating honey bee foraging preferences. PLoS ONE 10(8), article number: e0134735. (10.1371/journal.pone.0134735)
- Buyuk, F. et al. 2015. The effect of prolonged storage on the virulence of isolates of Bacillus anthracis obtained from environmental and animal sources in the Kars Region of Turkey. FEMS Microbiology Letters 362(13), article number: fnv102. (10.1093/femsle/fnv102)
- Köhler, S. M., Baillie, L. and Beyer, W. 2015. BclA and toxin antigens augment each other to protect NMRI mice from lethal Bacillus anthracis challenge. Vaccine 33(24), pp. 2771-2777. (10.1016/j.vaccine.2015.04.049)
- Ingavle, G. C. et al. 2015. Affinity binding of antibodies to supermacroporous cryogel adsorbents with immobilized protein A for removal of anthrax toxin protective antigen. Biomaterials 50, pp. 140-153. (10.1016/j.biomaterials.2015.01.039)
- Ingram, R. J. et al. 2015. Natural cutaneous anthrax infection, but not vaccination, induces a CD4+ T cell response involving diverse cytokines. Cell & Bioscience 5, article number: 20. (10.1186/s13578-015-0011-4)
- Stedmon, A. W., Eachus, P., Baillie, L., Tallis, H., Donkor, R., Edlin-White, R. and Bracewell, R. 2015. Scalable interrogation: Eliciting human pheromone responses to deception in a security interview setting. Applied Ergonomics 47, pp. 26-33. (10.1016/j.apergo.2014.08.015)
- Joshi, L. T., Mali, B. L., Geddes, C. D. and Baillie, L. 2014. Extraction and sensitive detection of toxins A and B from the human pathogen clostridium difficile in 40 seconds using microwave-accelerated metal-enhanced fluorescence. PLoS ONE 9(8), article number: e104334. (10.1371/journal.pone.0104334)
- Ascough, S. et al. 2014. Anthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T Cell immunity. PLoS Pathogens 10(5), article number: e1004085. (10.1371/journal.ppat.1004085)
- Eachus, P., Stedmon, A. and Baillie, L. 2013. Hostile intent in public crowded spaces: A field study. Applied Ergonomics 44(5), pp. 703-709. (10.1016/j.apergo.2012.05.009)
- Ingram, R. J. et al. 2013. Exposure to anthrax toxin alters human leukocyte expression of Anthrax toxin receptor 1. Clinical and Experimental Immunology 173(1), pp. 84-91. (10.1111/cei.12090)
- Baillie, L. 2013. Can one size fit all? Towards a universal anthrax vaccine [Editorial]. Future Microbiology 8(3), pp. 295-297. (10.2217/fmb.13.11)
- Williams, G. J., Linley, E., Nicholas, R. and Baillie, L. 2013. The role of the exosporium in the environmental distribution of anthrax. Journal of Applied Microbiology 114(2), pp. 396-403. (10.1111/jam.12034)
- Zhang, J. et al. 2013. An adenovirus-vectored nasal vaccine confers rapid and sustained protection against Anthrax in a single-dose regimen. Clinical and Vaccine Immunology 20(1), pp. 1-8. (10.1128/CVI.00280-12)
- Joshi, L. T., Phillips, D. S., Williams, C. F., Alyousef, A. and Baillie, L. 2012. Contribution of Spores to the Ability of Clostridium difficile To Adhere to Surfaces. Applied and Environmental Microbiology 78(21), pp. 7671-7679. (10.1128/AEM.01862-12)
- Ascough, S. et al. 2012. Identification of immunodominant T cell epitopes from anthrax protective antigen for inclusion in a rationally designed sub-unit based vaccine [Abstract]. Immunology 137(S1), pp. 761. (10.1111/imm.12002)
- Albrecht, M. T., Eyles, J. E., Baillie, L. and Keane-Myers, A. M. 2012. Immunogenicity and efficacy of an anthrax/plague DNA fusion vaccine in a mouse model. FEMS Immunology & Medical Microbiology 65(3), pp. 505-509. (10.1111/j.1574-695X.2012.00974.x)
- Ingram, R. and Baillie, L. 2012. It's in the genes! Human genetic diversity and the response to anthrax vaccines [Editorial]. Expert Review of Vaccines 11(6), pp. 633-635. (10.1586/erv.12.41)
- Brenneman, K. E. et al. 2011. The early humoral immune response to Bacillus anthracis toxins in patients infected with cutaneous anthrax. FEMS Immunology & Medical Microbiology 62(2), pp. 164-172. (10.1111/j.1574-695X.2011.00800.x)
- Mett, V. et al. 2011. A non-glycosylated, plant-produced human monoclonal antibody against anthrax protective antigen protects mice and non-human primates from B. anthracis spore challenge. Human Vaccines 7, pp. 183-190. (10.4161/hv.7.0.14586)
- Porasuphatana, S. et al. 2010. Bacillus Anthracis Endospores Regulate Ornithine Decarboxylase and Inducible Nitric Oxide Synthase Through ERK1/2 and p38 Mitogen-Activated Protein Kinases. Current Microbiology 61(6), pp. 567-573. (10.1007/s00284-010-9654-x)
- Baillie, L. et al. 2010. An anthrax subunit vaccine candidate based on protective regions of Bacillus anthracis protective antigen and lethal factor. Vaccine 28(41), pp. 6740-6748. (10.1016/j.vaccine.2010.07.075)
- Ramirez, K., Ditamo, Y., Galen, J. E., Baillie, L. and Pasetti, M. F. 2010. Mucosal priming of newborn mice with S. Typhi Ty21a expressing anthrax protective antigen (PA) followed by parenteral PA-boost induces B and T cell-mediated immunity that protects against infection bypassing maternal antibodies. Vaccine 28(37), pp. 6065-6075. (10.1016/j.vaccine.2010.06.089)
- Ingram, R. J. et al. 2010. An Epitope of Bacillus anthracis Protective Antigen That Is Cryptic in Rabbits May Be Immunodominant in Humans [Letter]. Infection and Immunity 78(5), pp. 2353-2354. (10.1128/IAI.00072-10)
- Ingram, R. J. et al. 2010. Natural exposure to cutaneous anthrax gives long-lasting T cell immunity encompassing infection-specific epitopes. Journal of Immunology 184(7), pp. 3814-3821. (10.4049/jimmunol.0901581)
- Vernazza, C., Lingard, B., Flick-Smith, H. C., Baillie, L., Hill, J. and Atkins, H. S. 2009. Small protective fragments of the Yersinia pestis V antigen. Vaccine 27(21), pp. 2775-2780. (10.1016/j.vaccine.2009.03.011)
- Baillie, L., Rodriguez, A. L., Moore, S., Atkins, H. S., Feng, C., Nataro, J. P. and Pasetti, M. F. 2008. Towards a human oral vaccine for anthrax: the utility of a Salmonella Typhi Ty21a-based prime-boost immunization strategy. Vaccine 26(48), pp. 6083-6091. (10.1016/j.vaccine.2008.09.010)
- Aslan, K., Previte, M. J. R., Zhang, Y. X., Gallagher, T., Baillie, L. and Geddes, C. D. 2008. Extraction and detection of DNA from Bacillus anthracis spores and the vegetative cells within 1 min. Analytical Chemistry (including News & Features) 80(11), pp. 4125-4132. (10.1021/ac800519r)
- Kang, T. J., Basu, S., Zhang, L., Thomas, K. E., Vogel, S. N., Baillie, L. and Cross, A. S. 2008. Bacillus anthracis spores and lethal toxin induce IL-1 beta via functionally distinct signaling pathways. European Journal of Immunology 38(6), pp. 1574-1584. (10.1002/eji.200838141)
- Basu, S., Kang, T. J., Chen, W. H., Fenton, M. J., Baillie, L., Hibbs, S. and Cross, A. S. 2007. Role of Bacillus anthracis spore structures in macrophage cytokine responses. Infection and Immunity 75(5), pp. 2351-2358. (10.1128/IAI.01982-06)
- Stokes, M. G. M. et al. 2007. Oral administration of a Salmonella enterica-based vaccine expressing Bacillus anthracis protective antigen confers protection against aerosolized B. anthracis. Infection and Immunity 75(4), pp. 1827-1834. (10.1128/IAI.01242-06)
- Albrecht, M. T. et al. 2007. Human monoclonal antibodies against anthrax lethal factor and protective antigen act independently to protect against Bacillus anthracis infection and enhance endogenous immunity to anthrax. Infection and Immunity 75(11), pp. 5425-5433. (10.1128/iai.00261-07)
- Aslan, K., Previte, M. J. R., Zhang, Y. X., Baillie, L. and Geddes, C. D. 2007. Ultra-fast and sensitive DNA hybridization assays: Application to genomic anthrax detection in < 30 seconds. Biophysical Journal, pp. 552A-552A.
- Aslan, K., Zhang, Y. X., Hibbs, S., Baillie, L., Previte, M. J. R. and Geddes, C. D. 2007. Microwave-accelerated metal-enhanced fluorescence: application to detection of genomic and exosporium anthrax DNA in < 30 seconds. Analyst 132(11), pp. 1130-1138. (10.1039/b707876e)
- Basu, S., Kang, T. J., Chen, W. H., Fenton, M. J., Baillie, L., Hibbs, S. and Cross, A. S. 2007. Role of Bacillus anthracis spore structures in macrophage cytokine responses. Infection and Immunity 75(5), pp. 2351-2358. (10.1128/iai.01982-06)
- Hepburn, M. J. et al. 2007. Immune response to two different dosing schedules of the anthrax vaccine precipitated (AVP) vaccine. Vaccine 25(32), pp. 6089-6097. (10.1016/j.vaccine.2007.05.018)
- Legutki, J. B., Nelson, M., Titball, R., Galloway, D. R., Mateczun, A. and Baillie, L. 2007. Analysis of peptide mimotopes of Burkholderia pseudomallei exopolysaccharide. Vaccine 25(45), pp. 7796-7805. (10.1016/j.vaccine.2007.08.045)
- Stokes, M. G. M. et al. 2007. Oral administration of a Salmonella enterica-based vaccine expressing Bacillus anthracis protective antigen confers protection against aerosolized B-anthracis. Infection and Immunity 75(4), pp. 1827-1834. (10.1128/iai.01242-06)
- Weaver, J. et al. 2007. The protective role of bacillus anthracis exosporium in macrophage-mediated killing by nitric oxide. The FASEB Journal 21(5), pp. A624-A624.
- Weaver, J. et al. 2007. Protective role of Bacillus anthracis exosporium in macrophage-mediated killing by nitric oxide. Infection and Immunity 75(8), pp. 3894-3901. (10.1128/iai.00283-07)
- Priest, F. G., Barker, M., Baillie, L., Holmes, E. C. and Maiden, M. C. J. 2004. Population. Journal of Bacteriology 186(23), pp. 7959-7970. (10.1128/JB.186.23.7959-7970.2004)
- Baillie, L., Peterson, S., Read, T. and Tourasse, N. 2003. The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria. Nature 423(6935), pp. 81-86. (10.1038/nature01586)
- Baillie, L., Hebdon, R., Flick-Smith, H. and Williamson, D. 2003. Characterisation of the immune response to the UK human anthrax vaccine. FEMS Immunology and Medical Microbiology 36(1-2), pp. 83-86. (10.1016/S0928-8244(03)00085-3)
- Williams, R. C. et al. 2003. Production of Bacillus anthracis protective antigen is dependent on the extracellular chaperone, PrsA. Journal of Biological Chemistry 278(20), pp. 18056-18062. (10.1074/jbc.M301244200)
- Williamson, E. D., Bennett, A. M., Perkins, S. D., Beedham, R. J., Miller, J. and Baillie, L. 2002. Co-immunisation with a plasmid DNA cocktail primes mice against anthrax and plague. Vaccine 20(23-24), pp. 2933-2941. (10.1016/S0264-410X(02)00232-3)
- Flick-Smith, H. C. et al. 2002. Mucosal or parenteral administration of microsphere-associated Bacillus anthracis protective antigen protects against anthrax infection in mice. Infection and Immunity 70(4), pp. 2022-2028. (10.1128/IAI.70.4.2022-2028.2002)
- Thwaite, J. E., Baillie, L., Carter, N. M., Stephenson, K., Rees, M., Harwood, C. R. and Emmerson, P. T. 2002. Optimization of the cell wall microenvironment allows increased production of recombinant Bacillus anthracis protective antigen from B-subtilis. Applied and Environmental Microbiology 68(1), pp. 227-234. (10.1128/AEM.68.1.227-234.2002)
- Baillie, L. 2001. The development of new vaccines against Bacillus anthracis. Journal of Applied Microbiology 91(4), pp. 609-613. (10.1046/j.1365-2672.2001.01498.x)
- Baillie, L. and Godfree, A. 2001. Dangerous Pathogens 2000, University of Plymouth, 4-7th September 2000 [Preface]. Journal of Applied Microbiology 91(4), pp. 571. (10.1046/j.1365-2672.2001.01307.x)
- Williamson, E. D., Beedham, R. J., Bennett, A. M., Perkins, S. D., Miller, J. and Baillie, L. 1999. Presentation of protective antigen to the mouse immune system: immune sequelae. Journal of Applied Microbiology 87(2), pp. 315-317. (10.1046/j.1365-2672.1999.00901.x)
- Zegers, N. D., Kluter, E., van der Stap, H., van Dura, E., van Dalen, P., Shaw, M. and Baillie, L. 1999. Expression of the protective antigen of Bacillus anthracis by Lactobacillus casei: towards the development of an oral vaccine against anthrax. Journal of Applied Microbiology 87(2), pp. 309-314. (10.1046/j.1365-2672.1999.00900.x)
- Baillie, L., Fowler, K. and Turnbull, P. C. B. 1999. Human immune responses to the UK human anthrax vaccine. Journal of Applied Microbiology 87(2), pp. 306-308. (10.1046/j.1365-2672.1999.00899.x)
- Fowler, K., McBride, B. W., Turnbull, P. C. B. and Baillie, L. 1999. Immune correlates of protection against anthrax. Journal of Applied Microbiology 87(2), pp. 305. (10.1046/j.1365-2672.1999.00898.x)
- Baillie, L., Moore, P. and McBride, B. W. 1998. A heat-inducible Bacillus subtilis bacteriophage Phi 105 expression system for the production of the protective antigen of Bacillus anthracis. FEMS Microbiology Letters 163(1), pp. 43-47. (10.1111/j.1574-6968.1998.tb13024.x)
- McBride, B., Mogg, A., Telfer, J. L., Lever, M. S., Miller, J., Turnbull, P. C. B. and Baillie, L. 1998. Protective efficacy of a recombinant protective antigen against Bacillus anthracis challenge and assessment of immunological markers. Vaccine 16(8), pp. 810-817. (10.1016/S0264-410X(97)00268-5)
- Baillie, L., Moir, A. and Manchee, R. 1998. The expression of the protective antigen of Bacillus anthracis in Bacillus subtilis. Journal of Applied Microbiology 84(5), pp. 741-746. (10.1046/j.1365-2672.1998.00405.x)
- Miller, J., McBride, B. W., Manchee, R. J., Moore, P. and Baillie, L. 1998. Production and purification of recombinant protective antigen and protective efficacy against Bacillus anthracis. Letters in Applied Microbiology 26(1), pp. 56-60. (10.1046/j.1472-765X.1998.00274.x)
- Baillie, L., Jones, M. N., Turnbull, P. C. B. and Manchee, R. J. 1995. Evaluation of the Biolog system for the identification of Bacillus anthracis. Letters in Applied Microbiology 20(4), pp. 209-211. (10.1111/j.1472-765X.1995.tb00429.x)
- Baillie, L., Johnson, M. and Manchee, R. J. 1994. Evaluation of Bacillus subtilis strain IS53 for the production of Bacillus anthracis protective antigen. Letters in Applied Microbiology 19(4), pp. 225-227. (10.1111/j.1472-765X.1994.tb00949.x)
- Baillie, L., Wade, J. J. and Casewell, M. W. 1993. Colonial variation in vancomycin resistant Enterococcus faecium. Journal of Clinical Pathology 46(5), pp. 474-475. (10.1136/jcp.46.5.474)
- Wade, J., Baillie, L., Rolando, N. and Casewell, M. 1992. Pristinamycin for Enterococcus faecium resistant to vancomycin and gentamicin. Lancet 339(8788), pp. 312-313. (10.1016/0140-6736(92)91391-K)
- Baillie, L. 1990. Rapid identification of Klebsiella [Letter]. Journal of Clinical Pathology 43(10), pp. 875. (10.1136/jcp.43.10.875-b)
- Baillie, L. 1989. Ciproflaxacin-resistant pseudomonas-aeruginosa strain. Medical Laboratory Sciences 46(1), pp. 92-93.
- Baillie, L. 1987. A survey of the incidence of penicillin-resistant beta-lactamase-negative strains of staphylococcus-aureus. Medical Laboratory Sciences 44(3), pp. 285-286.
- Baillie, L. 1987. Chlorhexidine resistance among bacteria isolated from urine of catheterized patients [Short report]. Journal of Hospital Infection 10(1), pp. 83-86. (10.1016/0195-6701(87)90037-5)
- Baillie, L. 1986. Routine screening of catheter urine specimens for chlorhexidine resistant organisms. Medical Laboratory Sciences 43(3), pp. 284-285.
Book sections
- Gallagher, T. and Baillie, L. 2013. Anthrax in zoonoses. In: Palmer, S. R. et al. eds. Oxford Textbook of Zoonoses Biology, Clinical Practice, and Public Health Control. Second Edition. Oxford Textbooks In Public Health Oxford University Press
- Baillie, L. and Theriault, S. 2013. The control of biological agents. In: Fraise, A. P., Maillard, J. and Satar, S. eds. Principles and Practice of Disinfection, Preservation and Sterilization. Wiley-Blackwell, pp. 576.
- Baillie, L. and Theriault, S. 2012. Control of infectious bioagents. In: Fraise, A., Maillard, J. and Sattar, S. eds. Russell, Hugo & Ayliffe's Principles and Practice of Disinfection, Preservation & Sterilization. Oxford: Wiley-Blackwell, pp. 576-588., (10.1002/9781118425831.ch23)
- Baillie, L., Dyson, H. and Simpson, A. 2011. Dual use of biotechnology. In: Singer, P., Callahan, D. and Chadwick, R. F. eds. Encyclopedia of Applied Ethics. London: Academic Press Inc
- Baillie, L. 2000. Bacillus anthracis. In: Batt, C. A., Robinson, R. K. and Patel, P. D. eds. The Encyclopaedia of Food Microbiology. Academic Press, pp. 129-135.
Conferences
- Wilson-Garner, S., Baillie, L. and Porch, A. 2023. Increasing sample volume for microwave-assisted rapid DNA release. Presented at: 2022 Asia-Pacific Microwave Conference (APMC), Yokohama, Japan, 29 November 2022 - 02 December 2022Proceedings of 2022 Asia-Pacific Microwave Conference (APMC). IEEE pp. 638-640., (10.23919/APMC55665.2022.10000050)
- Hamzah, H., Ahortor, E., Malyshev, D., Choi, H., Lees, J., Baillie, L. and Porch, A. 2019. A compact microwave applicator for the rapid detection of clostridium difficile. Presented at: 2019 IEEE MTT-S International Microwave Biomedical Conference (IMBioC), Nanjing, China, 6-8 May 20192019 IEEE MTT-S International Microwave Biomedical Conference (IMBioC). IEEE, (10.1109/IMBIOC.2019.8777882)
- Moore, A., Davies, T., Berube, K., Jones, T. and Baillie, L. 2018. Bio-reactive clay minerals and anthrax decontamination: a novel antimicrobial solution. Presented at: Focused Meeting on Emerging Zoonoses and Antimicrobial Resistance, School of Veterinary Medicine, University of Surrey, Guildford, UK, 2 July 2018.
- Centeleghe, I., Myles, E., Baillie, L., Berube, K., Jones, T. and Blaxland, J. 2018. Combating the rising global threat of antimicrobial resistance with clay minerals: A study on two major hospital superbugs. Presented at: Focused meeting 2018: Emerging zoonoses and AMR: A global threat, University of Surrey, 02-02 July 2018.
Ymchwil
Cymwysterau
Gadewais Brifysgol Plymouth ym 1982 â Baglor yn y Gwyddorau. Yn dilyn astudio’n rhan amser, gadewais Brifysgol Gorllewin Lloegr ym 1991 â MPhil a Phrifysgol Sheffield yn 2001 â PhD.
Prosiectau pwysig
Rwy’n rhan o brosiect biosynwyryddion rhyngwladol sy’n cael ei gyllido gan NATO (2021) a’i arwain gan Brifysgol Caerdydd o'r enw ‘Synhwyrydd amser real newydd sy’n seiliedig ar nanoronynnau ar gyfer B. anthracis a M. twbercwlosis’. Mae’r tîm yn cynnwys sefydliadau yn yr Wcráin (Sefydliad Geocemeg Amgylcheddol y Wladwriaeth, Sefydliad Cemeg Arwyneb Chuiko a Chanolfan Glinigol a Phroffylactig "Phthisioleg" Cyngor Rhanbarthol Dnipropetrivsk) a'r Eidal (Istituto Zooprofilattico Sperimenttale dell Puglia e dell Basillicata).
Biosynhwyrydd ar gyfer canfod twbercwlosis sy'n gwrthsefyll gwrthfiotigau yn gyflym mewn poblogaethau crwydrol Affrica
Mae twbercwlosis yn heintio traean o boblogaeth y byd. Bydd y prosiect hwn yn hyrwyddo datblygiad prawf syml amser real (15 munud), cludadwy, cost isel, sy'n gallu gwneud diagnosis o'r clefyd mewn lleoliadau anghysbell yn Affrica. Mae hwn yn gyfle unigryw i weithio gyda pheirianwyr, biolegwyr a chlinigwyr i leihau dioddefaint pobl.
Cysylltwch â'r Athro Les Baillie i gael mwy o fanylion am y prosiect.
Diddordebau ymchwil
Esblygiad, ecoleg a rôl bacterioffagau wrth drosglwyddo genynnau llorweddol
Fy namcaniaeth weithredol yw bod B. anthracis wedi esblygu o straen o B.cereus trwy gaffael ffactorau ffyrnigrwydd o bacilli eraill yn llorweddol. Gan ddefnyddio nifer o ddulliau moleciwlaidd, rydym wedi cynhyrchu cryn dipyn o ddata i gefnogi'r rhagdybiaeth hon. Mae presenoldeb proffagau gwarchodedig yng ngenom pob unigyn B.anthracis a archwiliwyd hyd yma (> 300 unigion) yn awgrymu bod bacterioffagau, yn ogystal â phlasmidau, yn chwarae rôl wrth drosglwyddo genynnau ac esblygiad. Bydd ein hynysedd diweddar o bacterioffagau sy'n gallu heintio B.anthracis ac aelodau eraill o'r grŵp B.cereus yn galluogi eu defnyddio i bennu'r amodau amgylcheddol y mae trosglwyddo genynnau yn digwydd
Canfod Anthrax
O ystyried y bygythiad a berir gan B. anthracis yng nghyd-destun Bioderfysgaeth, mae angen datblygu profion canfod ar frys sy'n gallu canfod sborau yn yr amgylchedd a gwneud diagnosis o haint. Byddai prawf delfrydol yn benodol iawn, gellid ei ddefnyddio heb fawr o baratoi a fawr ddim offer ategol. Byddai hefyd yn rhoi canlyniadau cyflym mewn <60 eiliad, yn sefydlog iawn ar dymheredd yr ystafell a gellid ei ddefnyddio dro ar ôl tro. Mewn cydweithrediad â chydweithwyr yn adran Microbioleg ac Imiwnoleg Prifysgol Maryland yn Baltimore rydym yn gweithio i ddatblygu gwrthgyrff cadwyn sengl sefydlog thermol gan siarcod ar gyfer canfod tocsin a sborau anthracs. Dangoswyd bod gwrthgyrff a gynhyrchir gan siarcod yn cynnal capasiti rhwymo eu gwrthgyrff yn dilyn triniaeth wres hirfaith gan godi'r posibilrwydd o ddatblygu profion hynod sefydlog (Stanfield et al., 2004). Mewn cydweithrediad â Dr Chris Geddes, cyd-aelod cyfadran yn MBC rydym hefyd yn datblygu profion yn seiliedig ar ffflworoleuedd wedi'i wella â metel a all ganfod lefelau nanogram o fiomarcwyr anthracs mewn gwaed dynol mewn cyn lleied â 30 eiliad.
Pathogenigrwydd Anthrax
Mae gen i ddiddordeb arbennig mewn deall beth sy’n digwydd i’r celloedd yn dilyn heintiad macroffagau gan sborau B.anthracis. Mae fy nata diweddar yn awgrymu, er bod y sborau yn sbarduno nifer o dderbynyddion adnabod patrwm pathogen, ei fod yn dal i allu wella mecanweithiau lladd gwrthfacterol fel ocsid nitrig. Yn dilyn egino mewngellol llwyddiannus mae'r organeb yn mynegi rhwydwaith cymhleth o ffactorau ffyrnigrwydd sy'n ei alluogi i ddianc o'r gell. Mae gen i ddiddordeb tymor hir mewn deall y mecanweithiau sy'n rheoleiddio mynegiant ffactor ffyrnigrwydd in vivo ac ar hyn o bryd rwy'n ymchwilio i rôl regulon ffyrnigrwydd PlcR cymelladwy gydag ymchwilwyr blaenllaw o’r UD a Rwsia sydd wedi'u lleoli ar gampws NIH ym Methesda, Maryland.
Anthrax - Ymatebion imiwnedd yr organeb letyol
Deall ymateb imiwn unigolion wedi'u himiwneiddio a'u heintio fel dull o adnabod mecanweithiau amddiffyn. Mewn cydweithrediad â chlinigwyr yn Nhwrci lle mae anthrax yn endemig, rydym wedi nodweddu ymateb imiwnedd unigolion wedi’u heintio ac wedi'u himiwneiddio. Rydym wedi dangos mai gwrthgyrff yw prif gyfryngwr amddiffyniad. Rydw i’n gynghorydd gwyddonol ar gyfer dau gwmni rhyngwladol sy'n datblygu therapïau sy'n seiliedig ar wrthgyrff ar hyn o bryd. Rydw i hefyd yn ymchwilio i rôl celloedd cof B dynol gyda chydweithwyr yn Ysgol Meddygol Emory yn Atlanta. Hefyd, rydw i'n gweithio ar brosiect gyda Gweinyddiaeth Amddiffyn y DU i optimeiddio amserlen imiwneiddio’r brechlyn yn y DU. Yn olaf, rydw i'n ymwneud â phrosiect amlwladol a ariennir gan NIH yr UD dan arweiniad Coleg Imperial Llundain i ddatblygu brechlynnau DNA sy'n mynegi epitopau celloedd B a CD4 T sy'n rhoi amddiffyniad rhag anthrax a’r pla.
Brechlynnau rhag Anthrax
Mae datblygu brechlynnau rhag anthrax wedi bod yn ganolbwynt i fy ngyrfa ymchwil. Rwyf wedi datblygu dau frechlyn anthrax, un yn seiliedig ar brotein ailgyfunol (Gweinyddiaeth Amddiffyn y DU) a'r llall yn frechlyn DNA (Llynges yr UD) ac mae'r ddau ohonynt yn cael eu treialu’n glinigol. Mae fy ymdrech bresennol yn canolbwyntio ar ddatblygu ffyrdd o ddosbarthu brechlynnau heb nodwydd fel micro-mewngapsiwleiddio a defnyddio math gwan o Salmonela sy'n gallu rhoi amddiffyniad ar ôl dos drwy’r geg.
Gwrthgyrff therapiwtig
Gall gwrthgyrff parod rhoi amddiffyniad yn erbyn asiantau heintus yn syth. Gan weithio gyda chydweithwyr yn yr Iseldiroedd rydym wedi llwyddo i ynysu gwrthgyrff monoclonaidd dynol oddi wrth bobl wedi'u himiwneiddio ac wedi llwyddo i ddangos eu gallu i roi amddiffyniad mewn modelau anifeiliaid. Yn ogystal, rydym wedi datblygu systemau planhigion sy'n mynegi gwrthgyrff dynol fel system gynhyrchu cost isel.
Myfyrwyr presennol
Cyn-fyfyrwyr
Dr James Blaxland BSc - Ionawr 2011 - Rhagfyr 2014
Prosiect ar y cyd rhwng Fferylliaeth a ProTEM ServicesHops fel triniaeth bosibl ar gyfer dwbercwlosis mewn gwartheg a nwyon tŷ gwydr. Mae twbercwlosis yn cael ei achosi gan y bacteriwm Mycobacterium tuberculosis ac mae'n gyfrifol am fwy o farwolaethau nag unrhyw facteriwm arall. Mae perthynas agos iddo, Mycobacterium bovis yn gyfrifol am dwbercwlosis mewn gwartheg, clefyd cyfatebol mewn anifeiliaid, a all ledaenu i fodau dynol trwy gynhyrchion llaeth halogedig. Mae'r afiechyd yn bygwth cynnyrch amaethyddol a gall gael effaith ddramatig ar gyflenwadau bwyd a chymunedau gwledig yng Nghymru a gweddill y DU. Yn ddiweddar rhyddhaodd Llywodraeth Cynulliad Cymru ystadegau yn dangos bod y gost i’r trethdalwyr mewn iawndal i berchnogion gwartheg wedi cyrraedd £100 miliwn yn ystod y 10 mlynedd diwethaf. Yn wir, dangosodd gweinidog materion gwledig Cymru, Elin Jones, rhwng Ionawr a Hydref 2010, bod 6,587 o wartheg wedi’u lladd yng Nghymru oherwydd TB mewn gwartheg.
Myfyrwyr diweddar - Miss Jennifer Hawkins BSc - Hydref 2011 - Hydref 2014
Fel rhan o PhD Jenny Hawkins fe wnaethom ddatblygu dull wedi'i seilio ar DNA a oedd yn caniatáu i ni nodi'r planhigion a oedd wedi cyfrannu at wneud sampl fêl benodol. Ar ôl ei ddatblygu, gwnaethom ddefnyddio'r dull hwn ar samplau mêl yr oeddem wedi'u dangos o'r blaen yn cynnwys cyfansoddion gwrthfacterol sy'n deillio o blanhigion. Felly roeddem yn gallu adnabod y planhigion a oedd yn ffynhonnell wreiddiol y cyfansoddion a thrwy hynny eu targedu'n uniongyrchol fel ffynhonnell cyfansoddion newydd. Un o ddeilliannau'r gwaith hwn fu adnabod planhigion y mae gwenyn yn ymweld â nhw ac o ganlyniad gellir eu hystyried yn gyfeillgar i wenyn. Ar hyn o bryd rydym yn defnyddio'r wybodaeth hon i gefnogi nifer o brosiectau ledled Caerdydd i blannu planhigion sy'n gyfeillgar i wenyn gan gynnwys to canolfan siopa Dewi Sant. Gweler cyhoeddiad Jennifer isod:
Hawkins, J. et al. 2015. Using DNA metabarcoding to identify the floral composition of honey: a new tool for investigating honey bee foraging preferences. PLoS ONE 10(8), article number: e0134735. (10.1371/journal.pone.0134735) 
Prosiect ar y cyd rhwng Fferylliaeth a Gerddi Botaneg Cenedlaethol Cymru. Noddir gan Ysgoloriaethau Sgiliau'r Economi Wybodaeth (KESS2). Gwenyn Apothecari, defnyddio'r wenynen fêl i ddarganfod cyffuriau - Adroddiad gan y BBC
Mae’r dystiolaeth gynharaf o fodau dynol yn casglu mêl i’w gweld mewn paentiad ogof yn Valencia, ar arfordir dwyreiniol Sbaen, y credir ei fod yn dyddio o tua 8000 CC. Ers tua 4000 CC, amlinellodd theori feddygol hynafol Hindi Ayurveda rinweddau meddyginiaethol mêl wrth drin llosgiadau, alergeddau a heintiau. Mae diwylliannau'r gorllewin wedi dal i fyny yn y pen draw trwy ddyfeisio gorchuddion clwyfau a meddyginiaethau drwy’r geg yn defnyddio mêl. Ond mae cyfansoddiad mêl yn amrywio'n fawr, ac mae'n dibynnu ar y fflora lleol yn yr amgylchedd sydd union o gwmpas y gwenyn. Gan fod gwenyn yn ymweld â blodau amrywiol gyda gwahanol briodweddau iacháu wrth wneud mêl, mae'r cwmpas ar gyfer dod o hyd i ddefnyddiau newydd ar gyfer mêl yn enfawr. Ym Mhrifysgol Caerdydd, byddaf yn cynnal ymchwil er mwyn gweld a all mêl helpu i frwydro yn erbyn "archfygiau" a geir mewn ysbytai, y bacteria marwol sydd wedi datblygu sy'n gallu gwrthsefyll gwrthfiotigau confensiynol. Bydd fy astudiaeth yn defnyddio samplau a ddarperir gan wneuthurwyr mêl ledled y wlad ynghyd â rhestr o blanhigion ger eu cychod gwenyn. Bydd samplau amrwd, heb eu prosesu, yn cael eu sgrinio'n fanwl gan ddefnyddio profion a ddatblygwyd dros y tair blynedd. Defnyddir y profion hyn sy'n cynnwys taenu agar, gwanhau broth a phrofion dros amser i nodi'r mêl sydd â'r mwyaf o weithgaredd gwrthfacterol. Bydd y prosiect a ariennir gan KESS yn cynnwys profi effeithiau mêl yn erbyn dau o'r heintiau mwyaf cyffredin a gafwyd mewn ysbytai, bacteria sydd ag ymwrthedd i wrthfiotigau MRSA a Clostridium difficile.
Miss Lovleen Tina Joshi BSc - Hydref 2008 - Medi 2011
Prawf erchwyn gwely i ganfod Clostridium difficile yn ysgarthion cleifion yn yr ysbyty mewn amser real.
Nod y PhD hwn yw cynllunio prawf ar gyfer canfod sborau Clostridium difficile a chelloedd llystyfol o fewn 60 eiliad yn ysgarthion cleifion ysbyty. Ar hyn o bryd prin yw'r dulliau sy'n canfod dau docsin C. difficile yn gyflym gyda sensitifrwydd a phenodoldeb uchel. Felly bydd y ddyfais ddiagnostig arfaethedig yn canfod tocsinau ffyrnigrwydd A a B yn yr organeb gan ddefnyddio technoleg platfform newydd, sef Microwave- Accelerated Metal-Enhanced fluorescence (MAMEF) Cyflawnir hyn trwy nodi llofnodion genynnol hirsefydlog y ddau docsin a'u hymgorffori yn y prawf canfod. Bydd y ddyfais ganfod yn cynorthwyo clinigwyr i wneud diagnosis a thrin cleifion â haint C. difficile a'r cleifion hynny sydd â'r potensial i ddatblygu haint.
Mr Abdullah Alyousef MSc - Ebrill 2009- Mawrth 2012
Ynysu a nodweddu bacterioffagau lytig ar gyfer trin Clostridium difficile.
Mae bacterioffagau (feirysau sy'n targedu bacteria yn benodol) wedi cael eu defnyddio'n llwyddiannus ers degawdau yn yr hen Undeb Sofietaidd i drin afiechydon heintus, yn aml yn hytrach na gwrthfiotigau. Mewn cyferbyniad, mae gwledydd y gorllewin wedi defnyddio gwrthfiotigau i drin heintiau tebyg yn draddodiadol. O ganlyniad rydym wedi gweld ymddangosiad micro-organebau fel Staphylococcus aureus (MRSA) sy'n ymwrthol i Methisilin ac sy'n gwrthsefyll mwyafrif y cyffuriau sydd ar gael yn fasnachol. Mae problem archfygiau sy'n ymwrthol i gyffuriau yn ein hysbytai wedi ysgogi ymchwilwyr i edrych eto ar fudd defnyddio bacterioffagau (phages) i drin heintiau a achosir gan yr organebau hyn.
Rydym yn cynnig adnabod bacterioffagau sy'n gallu targedu ac anactifadu Clostridium difficile, sef bacteriwm sy’n achosi haint gastrig gwanychol cleifion yn yr ysbyty, sydd wedi bod yn gyfrifol am forbidrwydd a marwolaethau sylweddol ymysg cleifion yng Nghymru. Mae rhagolwg o'r costau gofal iechyd blynyddol dolur rhydd yn ymwneud â C.difficile (CDAD) Cymru yn fwy na £10 miliwn, gyda'r niferoedd uchaf yn cael eu hadrodd o arbenigeddau meddygol cyffredinol a geriatrig. Er ein bod yn gwybod bod defnyddio gwrthfiotigau penodol, ffactor sy'n cyfrannu at niferoedd cynyddol o achosion ymysg cleifion yn yr ysbyty yw gallu'r bacteriwm i greu sborau. Mae'r rhain yn galluogi'r organeb i fod yn hyfyw am fisoedd ar arwynebau wedi'u halogi yn yr ysbyty, hyd yn oed ar ôl glanhau gyda diheintydd. Nododd astudiaeth ym 1996 bod 20% o'r samplau amgylcheddol o wardiau ysbyty yng Nghaerdydd wedi profi'n bositif am C.difficile. Mae hyn yn arwyddocaol o ystyried bod arunigion amgylcheddol wedi cael y bai am ledaenu CDAD drwy ddwylo gweithwyr gofal iechyd.
Byddai'r gallu i drin unigolion sy'n ddifrifol wael, a diheintio eu hamgylchedd cyfagos, ac felly atal lledaeniad yr haint i gleifion eraill, yn cael effaith sylweddol ar ganlyniadau gofal iechyd a chostau hefyd. Mae bacterioffagau'n cynnig nifer o fanteision gan gynnwys diogelwch. Maent yn un o'r ffurfiau bywyd mwyaf cyffredin ar y blaned gyda hanes o ddefnydd diogel ymysg bodau dynol. Yn benodol, maent yn targedu un math o ficro-organeb ac yn gadael bacteria manteisiol i fod. Maent hefyd yn gweithio yn erbyn rhywogaethau sy'n ymwrthiol i wrthfiotigau.
Miss Harsha Siani BSc - Hydref 2008 - Medi 2011
Gwyddonydd Ymchwil a Rheolwr Labordy
Prosiect ar y cyd rhwng Ysgol Fferylliaeth Cymru, Prifysgol Caerdydd a Chorfforaeth IQ NL i ddatblygu therapi ar sail gwrthgorff ar gyfer Clostridium difficile
Mae Clostridium difficile wedi dod i’r fei fel yr achos mwyaf cyffredin o’r dolur rhydd nosocomial, sy’n costio $1 biliwn i system gofal iechyd yr UD bob blwyddyn, a £4000 i’r GIG drin pob achos. Dros £10 miliwn yw’r amcangyfrif o gost y dolur rhydd sy’n gysylltiedig â C. difficile (CDAD) i Gymru bob blwyddyn. Yn waeth fyth nag effaith economaidd yr haint, mae’r nifer o achosion CDAD yn cynyddu ar draws y byd bob blwyddyn, ac mae rhywogaeth ffyrnig dros ben wedi esblygu sy’n gyfrifol am darddiadau o achosion mwy difrifol yn Ewrop a Gogledd America.
Mr William McCully MRPharmS - Ionawr 2010 - Ionawr 2013
Prosiect ar y cyd rhwng Ysgol Fferylliaeth Cymru, Prifysgol Caerdydd a Gardd Fotaneg Genedlaethol Cymru. Dan nawdd rhaglen KESS. Therapiwtig Naturiol ar gyfer Pathogen Clostridium Difficile mewn Ysbytai
Mae te yn drwythiad dŵr poeth o’r dail o blanhigyn sinensis Camelia. Mae’n un o’r diodydd a yfir fwyaf ar draws y byd, ac yn y DU rydym yn yfed mwy o gwpanau o de y pen nag unrhyw wlad arall. Mae sawl amrywiaeth o de a’r rhai mwyaf cyffredin yw te du a the gwyrdd. Fodd bynnag, mae’r gwahanol amrywiaethau yn dod o’r un planhigyn. Yr unig beth sy’n eu gwneud yn wahanol yw’r dull a ddefnyddir i brosesu’r dail a dynnir. Mae te wedi’i ddefnyddio’n eang oherwydd ei briodweddau meddygol, yn enwedig mewn te Tsieineaidd. Mewn ymchwil wyddonol ddiweddar, mae wedi dod i’r amlwg bod priodweddau gwrthfacterol, gwrthganser a gwrthfeirysol mewn te. Tybir bod y priodweddau hyn yn deillio o grŵp o wrthocsidyddion mewn te o’r enw polyffenolau. Fe wnaethom ddarganfod yn ddiweddar yn Ysgol Fferylliaeth Cymru bod te yn atal ‘archfyg’ mewn ysbytai, Clostridium difficile, rhag tyfu.
Nod fy mhrosiect yw ceisio darganfod pa gydrannau mewn te sy’n gyfrifol am ei weithgarwch gwrthfacterol yn erbyn Clostridium difficile a deall sut mae’n gweithredu. Byddaf hefyd yn ceisio addasu’r amodau tyfu mewn planhigfa fach o blanhigion sinensis Camelia yng Ngerddi Botaneg Cenedlaethol Cymru er mwyn cynhyrchu ‘te rhagorol’ sydd â llawer o bolyffenolau a gweithgarwch gwrthfacterol. Gyda lwc, byddwn yn gallu cynhyrchu te wedi’i sydd wedi’i wella’n naturiol fydd yn glinigol effeithiol yn erbyn haint Clostridum difficile.
Addysgu
Addysgu israddedigion MPharm
- PH1122 Rôl y fferyllydd mewn ymarfer proffesiynol
- PH2112 Egwyddorion dylunio cyffuriau
- PH3202 Methodoleg ymchwil
- PH4116 Prosiect ysgoloriaeth neu ymchwil ym maes fferylliaeth
- PH4117 Gwyddorau fferyllol, ymarfer fferyllol a’r boblogaeth
Bywgraffiad
Proffil gyrfa
Ar hyn o bryd
- Athro Microbioleg, Ysgol Fferylliaeth a Gwyddorau Fferyllol ers 2007
- Athro Anrhydeddus, Prifysgol Heriot Watt ers 2006
- Athro Cyswllt, Cyfarwyddwr Menter Bioamddiffyn, Canolfan Biodechnoleg Feddygol, Sefydliad Biodechnoleg Prifysgol Maryland, Baltimore ers 2002
- Athro Cynorthwyol, Adran Microbioleg ac Imiwnoleg, Ysgol Meddygaeth Prifysgol Maryland, Baltimore ers 2003
Blaenorol
- Pennaeth Adran, Gwrthfesurau Bioamddiffyn Meddygol, Cyfarwyddiaeth Ymchwil Amddiffyn Biolegol, Canolfan Ymchwil Feddygol Forol yr UD, Washington DC o 2003 tan 2007
- Prif Wyddonydd, Gwyddorau Cemegol a Biolegol, Labordy Gwyddoniaeth a Thechnolegau Amddiffyn, Porton Down (y Weinyddiaeth Amddiffyn), Caersallog o 1993 tan 2002
Prif arbenigedd
Micro-organebau yng nghategori 3, sborau bacteriol, bacterioffagau, bioleg foleciwlaidd, mynegiad a systemau cyflwyno brechlynnau, brechlynnau DNA, profion gwrthgyrff, signalu imiwnedd, imiwnedd cynhenid
Meysydd goruchwyliaeth
Goruchwyliaeth gyfredol
Khalid Alyahya Alyahya
Myfyriwr ymchwil
Tawfiq Juraybi
Myfyriwr ymchwil
