![Helen Waller-Evans](https://profiles-cardiff.imgix.net/attachments/1245?w=200&h=200&auto=format&crop=faces&fit=crop&q=90")
Dr Helen Waller-Evans
Darlithydd
Ysgol y Biowyddorau
- Waller-EvansH@caerdydd.ac.uk
- +44 29225 11093
- Y Prif Adeilad, Ystafell 0.20B, Plas y Parc, Caerdydd, CF10 3AT
- Siarad Cymraeg
Trosolwyg
Rwy'n ddarlithydd niwrowyddoniaeth yn y Sefydliad Darganfod Meddyginiaethau. Er mwyn datblygu gwell cyffuriau niwrowyddoniaeth, mae angen i ni ddeall yn llawn rolau targedau cyffuriau posibl a'r llwybrau y maent yn ymwneud â nhw. Mae gwaith yn fy labordy yn canolbwyntio ar nodweddu swyddogaeth targedau cyffuriau niwrowyddoniaeth posibl a datblygu profion i asesu effeithiau cyffuriau newydd ar y swyddogaeth hon. Mae gen i ddiddordeb arbennig mewn datblygu therapïau sy'n gweithredu ar broteinau lysosomaidd i'w defnyddio mewn clefydau seiciatrig a niwrolegol.
Cyhoeddiad
2023
- Best, H. L., Williamson, L. J., Heath, E. A., Waller-Evans, H., Lloyd-Evans, E. and Berry, C. 2023. The role of glycoconjugates as receptors for insecticidal proteins. FEMS Microbiology Reviews 47(4) (10.1093/femsre/fuad026)
- Somogyi, A. et al. 2023. The synthetic TRPML1 agonist ML-SA1 rescues Alzheimer-related alterations of the endosomal-autophagic-lysosomal system. Journal of Cell Science 136(6), article number: jcs259875. (10.1242/jcs.259875)
- Fenn, G. D., Alenazy, D. M., Pope, S., Lloyd-Evans, E. and Waller-Evans, H. 2023. Development of a novel anthraquinone-derived fluorescent lysosomal probe. Molecular Genetics and Metabolism 138(2), article number: 107097. (10.1016/j.ymgme.2022.107097)
- Davies, M., Riseley, R., Jones, D. H. and Waller-Evans, H. 2023. Acid ceramidase inhibition as a mechanism to treat lysosomal disorders. Molecular Genetics and Metabolism 138(2), article number: 107069. (10.1016/j.ymgme.2022.107069)
- Best, H. L., Badell-Grau, R. A., Shipley, K., Alshehri, A. S., Waller-Evans, H. and Lloyd-Evans, E. 2023. Disease phenotypes present in CLN7 patient fibroblasts are improved following treatment to reduce glycosphingolipid synthesis.. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 17., (10.1016/j.ymgme.2022.107029)
- Waller-Evans, H., Francis, L., Fenn, G., Best, H. and Lloyd-Evans, E. 2023. Filipin composition and imaging modality dramatically affect cellular cholesterol visualisation with implications for assessing cholesterol levels and localisation across the lysosomal disorders. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 133., (10.1016/j.ymgme.2022.107352)
- Lloyd-Evans, E., Best, H. L., Alshehri, A. S., Honeybun, L. and Waller-Evans, H. 2023. Glycosphingolipid reduction with miglustat as a therapeutic strategy for CLN3 and other neuronal ceroid lipofuscinoses.. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 84., (10.1016/j.ymgme.2022.107212)
- Cook, S. R., Kirkham, E. D., Best, H., Waller-Evans, H., Serpi, M., Pertusati, F. and Lloyd-Evans, E. 2023. Investigating lysosomal and cellular phenotypes of the lysosomal disorder cystinosis. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 28-28., (10.1016/j.ymgme.2022.107061)
- Fenn, G. D., Lloyd-Evans, E. and Waller-Evans, H. 2023. Investigation of cannabidiol as a potential therapeutic in Niemann-Pick disease. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 42-42., (10.1016/j.ymgme.2022.107098)
- Waller-Evans, H., Zhu, J., Honeybun, L., Gardiner, S., Hanley, M., Best, H. and Lloyd-Evans, E. 2023. Measuring ion flux via lysosomal channels: Primary and secondary assays for drug discovery across the lysosomal disorders. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 133-134., (10.1016/j.ymgme.2022.107353)
- Honeybun, L., Waller-Evans, H. and Lloyd-Evans, E. 2023. Unbiased phenotypic drug screen for CLN3 disease. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 167-167., (10.1016/j.ymgme.2022.107150)
- Best, H., Honeybun, L., Waller-Evans, H. and Lloyd-Evans, E. 2023. The Batten disease associated protein CLN3 is required for the efflux of lysosomal K. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 47-47., (10.1016/j.ymgme.2022.107030)
2022
- Best, H. L., Williamson, L. J., Lipka-Lloyd, M., Waller-Evans, H., Lloyd-Evans, E., Rizkallah, P. J. and Berry, C. 2022. The crystal structure of Bacillus thuringiensis Tpp80Aa1 and its interaction with galactose-containing glycolipids. Toxins 14(12), article number: 863. (10.3390/toxins14120863)
2020
- Cook, S. R. et al. 2020. Visualisation of cholesterol and ganglioside GM1 in zebrafish models of Niemann-Pick type C disease and Smith-Lemli-Opitz syndrome using light sheet microscopy. Histochemistry and Cell Biology 154, pp. 565-578. (10.1007/s00418-020-01925-2)
- Badell Grau, R. A., Cuff, J. P., Kelly, B. P., Waller-Evans, H. and Lloyd-Evans, E. 2020. Investigating the prevalence of reactive online searching in the COVID-19 pandemic. Journal of Medical Internet Research 22(10), article number: e19791. (10.2196/19791)
- Fenn, G., Waller-Evans, H., Atack, J. R. and Bax, B. D. 2020. Crystallization and structure of ebselen bound to cysteine 141 of human inositol monophosphatase (IMPase). Acta Crystallographica Section F: Structural Biology Communications F76(10), pp. 469-476. (10.1107/S2053230X20011310)
- Lloyd-Evans, E. and Waller-Evans, H. 2020. Biosynthesis and signalling functions of central and peripheral nervous system neurosteroids in health and disease. Essays in Biochemistry 64(3), pp. 591-606. (10.1042/EBC20200043)
- Cook, S. R. et al. 2020. Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons. AMRC Open Research 2, article number: 21. (10.12688/amrcopenres.12903.1)
2018
- Morimoto, M. et al. 2018. Bi-allelic CCDC47 variants cause a disorder characterized by woolly hair, liver dysfunction, dysmorphic features, and global developmental delay. American Journal of Human Genetics 103(5), pp. 794-807. (10.1016/j.ajhg.2018.09.014)
2015
- Waller-Evans, H. and Lloyd-Evans, E. 2015. Regulation of TRPML1 function. Biochemical Society Transactions 43(3), pp. 442-446. (10.1042/BST20140311)
2014
- Kaisaki, P. J. et al. 2014. Genetic control of differential acetylation in diabetic rats. PLoS ONE 9(4), article number: e94555. (10.1371/journal.pone.0094555)
2013
- Waller-Evans, H. et al. 2013. Nutrigenomics of high fat diet induced obesity in mice suggests relationships between susceptibility to fatty liver disease and the proteasome. PLoS ONE 8(12), article number: e82825. (10.1371/journal.pone.0082825)
2012
- Prömel, S. et al. 2012. Characterization and functional study of a cluster of four highly conserved orphan adhesion-GPCR in mouse. Developmental Dynamics 241(10), pp. 1591-1602. (10.1002/dvdy.23841)
2010
- Lloyd-Evans, E., Waller-Evans, H., Peterneva, K. and Platt, F. M. 2010. Endolysosomal calcium regulation and disease.. Biochemical Society Transactions 38(6), pp. 1458-1464. (10.1042/BST0381458)
- Waller-Evans, H. et al. 2010. The orphan adhesion-GPCR GPR126 is required for embryonic development in the mouse. PLoS ONE 5(11), article number: e14047. (10.1371/journal.pone.0014047)
2009
- Langenhan, T. et al. 2009. Latrophilin signaling links anterior-posterior tissue polarity and oriented cell divisions in the C. elegans embryo. Developmental Cell 17(4), pp. 494-504. (10.1016/j.devcel.2009.08.008)
Articles
- Best, H. L., Williamson, L. J., Heath, E. A., Waller-Evans, H., Lloyd-Evans, E. and Berry, C. 2023. The role of glycoconjugates as receptors for insecticidal proteins. FEMS Microbiology Reviews 47(4) (10.1093/femsre/fuad026)
- Somogyi, A. et al. 2023. The synthetic TRPML1 agonist ML-SA1 rescues Alzheimer-related alterations of the endosomal-autophagic-lysosomal system. Journal of Cell Science 136(6), article number: jcs259875. (10.1242/jcs.259875)
- Fenn, G. D., Alenazy, D. M., Pope, S., Lloyd-Evans, E. and Waller-Evans, H. 2023. Development of a novel anthraquinone-derived fluorescent lysosomal probe. Molecular Genetics and Metabolism 138(2), article number: 107097. (10.1016/j.ymgme.2022.107097)
- Davies, M., Riseley, R., Jones, D. H. and Waller-Evans, H. 2023. Acid ceramidase inhibition as a mechanism to treat lysosomal disorders. Molecular Genetics and Metabolism 138(2), article number: 107069. (10.1016/j.ymgme.2022.107069)
- Best, H. L., Williamson, L. J., Lipka-Lloyd, M., Waller-Evans, H., Lloyd-Evans, E., Rizkallah, P. J. and Berry, C. 2022. The crystal structure of Bacillus thuringiensis Tpp80Aa1 and its interaction with galactose-containing glycolipids. Toxins 14(12), article number: 863. (10.3390/toxins14120863)
- Cook, S. R. et al. 2020. Visualisation of cholesterol and ganglioside GM1 in zebrafish models of Niemann-Pick type C disease and Smith-Lemli-Opitz syndrome using light sheet microscopy. Histochemistry and Cell Biology 154, pp. 565-578. (10.1007/s00418-020-01925-2)
- Badell Grau, R. A., Cuff, J. P., Kelly, B. P., Waller-Evans, H. and Lloyd-Evans, E. 2020. Investigating the prevalence of reactive online searching in the COVID-19 pandemic. Journal of Medical Internet Research 22(10), article number: e19791. (10.2196/19791)
- Fenn, G., Waller-Evans, H., Atack, J. R. and Bax, B. D. 2020. Crystallization and structure of ebselen bound to cysteine 141 of human inositol monophosphatase (IMPase). Acta Crystallographica Section F: Structural Biology Communications F76(10), pp. 469-476. (10.1107/S2053230X20011310)
- Lloyd-Evans, E. and Waller-Evans, H. 2020. Biosynthesis and signalling functions of central and peripheral nervous system neurosteroids in health and disease. Essays in Biochemistry 64(3), pp. 591-606. (10.1042/EBC20200043)
- Cook, S. R. et al. 2020. Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons. AMRC Open Research 2, article number: 21. (10.12688/amrcopenres.12903.1)
- Morimoto, M. et al. 2018. Bi-allelic CCDC47 variants cause a disorder characterized by woolly hair, liver dysfunction, dysmorphic features, and global developmental delay. American Journal of Human Genetics 103(5), pp. 794-807. (10.1016/j.ajhg.2018.09.014)
- Waller-Evans, H. and Lloyd-Evans, E. 2015. Regulation of TRPML1 function. Biochemical Society Transactions 43(3), pp. 442-446. (10.1042/BST20140311)
- Kaisaki, P. J. et al. 2014. Genetic control of differential acetylation in diabetic rats. PLoS ONE 9(4), article number: e94555. (10.1371/journal.pone.0094555)
- Waller-Evans, H. et al. 2013. Nutrigenomics of high fat diet induced obesity in mice suggests relationships between susceptibility to fatty liver disease and the proteasome. PLoS ONE 8(12), article number: e82825. (10.1371/journal.pone.0082825)
- Prömel, S. et al. 2012. Characterization and functional study of a cluster of four highly conserved orphan adhesion-GPCR in mouse. Developmental Dynamics 241(10), pp. 1591-1602. (10.1002/dvdy.23841)
- Lloyd-Evans, E., Waller-Evans, H., Peterneva, K. and Platt, F. M. 2010. Endolysosomal calcium regulation and disease.. Biochemical Society Transactions 38(6), pp. 1458-1464. (10.1042/BST0381458)
- Waller-Evans, H. et al. 2010. The orphan adhesion-GPCR GPR126 is required for embryonic development in the mouse. PLoS ONE 5(11), article number: e14047. (10.1371/journal.pone.0014047)
- Langenhan, T. et al. 2009. Latrophilin signaling links anterior-posterior tissue polarity and oriented cell divisions in the C. elegans embryo. Developmental Cell 17(4), pp. 494-504. (10.1016/j.devcel.2009.08.008)
Conferences
- Best, H. L., Badell-Grau, R. A., Shipley, K., Alshehri, A. S., Waller-Evans, H. and Lloyd-Evans, E. 2023. Disease phenotypes present in CLN7 patient fibroblasts are improved following treatment to reduce glycosphingolipid synthesis.. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 17., (10.1016/j.ymgme.2022.107029)
- Waller-Evans, H., Francis, L., Fenn, G., Best, H. and Lloyd-Evans, E. 2023. Filipin composition and imaging modality dramatically affect cellular cholesterol visualisation with implications for assessing cholesterol levels and localisation across the lysosomal disorders. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 133., (10.1016/j.ymgme.2022.107352)
- Lloyd-Evans, E., Best, H. L., Alshehri, A. S., Honeybun, L. and Waller-Evans, H. 2023. Glycosphingolipid reduction with miglustat as a therapeutic strategy for CLN3 and other neuronal ceroid lipofuscinoses.. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 84., (10.1016/j.ymgme.2022.107212)
- Cook, S. R., Kirkham, E. D., Best, H., Waller-Evans, H., Serpi, M., Pertusati, F. and Lloyd-Evans, E. 2023. Investigating lysosomal and cellular phenotypes of the lysosomal disorder cystinosis. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 28-28., (10.1016/j.ymgme.2022.107061)
- Fenn, G. D., Lloyd-Evans, E. and Waller-Evans, H. 2023. Investigation of cannabidiol as a potential therapeutic in Niemann-Pick disease. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 42-42., (10.1016/j.ymgme.2022.107098)
- Waller-Evans, H., Zhu, J., Honeybun, L., Gardiner, S., Hanley, M., Best, H. and Lloyd-Evans, E. 2023. Measuring ion flux via lysosomal channels: Primary and secondary assays for drug discovery across the lysosomal disorders. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 133-134., (10.1016/j.ymgme.2022.107353)
- Honeybun, L., Waller-Evans, H. and Lloyd-Evans, E. 2023. Unbiased phenotypic drug screen for CLN3 disease. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 167-167., (10.1016/j.ymgme.2022.107150)
- Best, H., Honeybun, L., Waller-Evans, H. and Lloyd-Evans, E. 2023. The Batten disease associated protein CLN3 is required for the efflux of lysosomal K. Presented at: 19th Annual WORLDSymposium™ 2023, Orlando, Florida, 21-26 February 2023, Vol. 138. Vol. 2. Elsevier pp. 47-47., (10.1016/j.ymgme.2022.107030)
Ymchwil
Diddordebau Ymchwil
Mae fy ymchwil yn canolbwyntio ar egluro swyddogaethau proteinau lysosomal a'u rolau mewn anhwylderau niwrolegol, a datblygu cyffuriau newydd sy'n targedu'r proteinau hyn i'w defnyddio yn y ddau glefydau o heneiddio ac anhwylderau storio lysosomal - clefydau genetig prin a achosir gan ddiffygion mewn proteinau lysosomal.
Fy mhrif ddiddordeb ymchwil yw'r protein NPC1, protein lysosomal sydd â rôl awgrymedig mewn trafnidiaeth colesterol. Mae colli swyddogaeth NPC1 yn asio clefyd math C Niemann-Select, anhwylder storio lysosomol, lle mae plentyn yr effeithir arno yn cael anhawster cerdded a llyncu, anawsterau dysgu, epilepsi a dementia. Gall y triniaethau presennol arafu dilyniant clefydau ond nid ydynt yn mynd i'r afael â'r diffyg gweithgarwch sylfaenol o NPC1. Rwy'n datblygu profion swyddogaethol ar gyfer protein lysosomal NPC1, a fydd yn caniatáu imi gynnal sgriniau cyffuriau ar gyfer moleciwlau a all actifadu NPC1 mewn cleifion math Niemann-Select.
Mae NPC1 hefyd yn cael ei ddefnyddio fel derbynnydd mewngellol gan nifer o firysau, gan gynnwys Ebola, hepatitis C, a coronafeirysau feline. Rwy'n ymchwilio i weld a all cyffuriau sy'n atal protein NPC1 atal SARS-CoV-2 rhag mynd i mewn i gelloedd, a allai alluogi eu hail-bwrpasu cyflym i drin Covid-19.
Aelodau presennol y Lab
Gareth Fenn - myfyriwr PhD
Sian Gardiner - uwch dechnegydd
Marcus Hanley - myfyriwr PhD
Bethany Tattersdill - cynorthwy-ydd ymchwil
Gabby Ward - technegydd
Cydweithredwyr
Dr Emyr Lloyd-Evans (Ysgol y Biowyddorau, Prifysgol Caerdydd)
Dr Ceri Fielding (Ysgol Meddygaeth, Prifysgol Caerdydd)
Yr Athro Simon Pope (Ysgol Cemeg, Prifysgol Caerdydd)
Yr Athro Colin Berry (Ysgol y Biowyddorau, Prifysgol Caerdydd)
Cyllid
Ariennir ymchwil yn fy labordy gan yr Academi Gwyddorau Meddygol, Sefydliad Ymchwil Niemann-Select, Llywodraeth Cymru, a Sefydliad Ymchwil Harrington.
Bywgraffiad
Hanes Gyrfa
2019 - presennol: Darlithydd niwrowyddoniaeth, Sefydliad Darganfod Meddyginiaethau, Prifysgol Caerdydd, lle byddaf yn datblygu cyffuriau newydd sy'n targedu proteinau lysosomal
2012 - 2019: Cydymaith Ymchwil gyda Dr Emyr Lloyd-Evans, Ysgol y Biowyddorau, Prifysgol Caerdydd. Yma, ymchwiliais i'r mecanweithiau sy'n arwain at bathogenesis mewn sawl anhwylder storio lysosomal, a dyma lle datblygais fy niddordeb mewn lysosomau.
2010 - 2012: Cydymaith Ymchwil gyda'r Athro Alun Davies FRS, Ysgol y Biowyddorau, Prifysgol Caerdydd. Yma, ymchwiliais i rôl ffactorau twf mewn datblygiad niwronau, a sut mae hyn yn cael ei effeithio mewn anhwylderau niwrolegol gan gynnwys clefyd Parkinson.
2008 - 2010: Cydymaith Ymchwil gyda'r Athro Dominique Gauguier, Prifysgol Rhydychen. Yma, ymchwiliais i gyfraniad gwahanol enynnau i ddifrifoldeb diabetes math 2.
2005 - 2008: PhD gyda Dr Andreas Russ, Prifysgol Rhydychen, yn ymchwilio i rôl GPR126, GPCR amddifad, yn natblygiad y llygoden.
2004 - 2005: Technegydd gyda Dr Andreas Russ, Prifysgol Rhydychen.
2003 - 2004: Diploma Ôl-raddedig mewn Seicoleg (rhagoriaeth), Prifysgol Oxford Brookes.
2002 - 2003: Histolegydd gyda'r Athro Nick Rawlins, Prifysgol Rhydychen.
1998 - 2002: BSc Anrh (dosbarth 1af) Bioleg Gymhwysol, Prifysgol Caerfaddon.
