Dr Mathieu Varache

Dr Mathieu Varache

Research Associate

School of Dentistry

Mae'r cynnwys hwn ar gael yn Saesneg yn unig.

My main interest lies in translational research that can be applied to humans. Especially, I am interested in developing and characterising nanomedicine-based delivery systems for therapeutic targeting of infectious and inflammatory diseases. With over six years of experience, I have a strong expertise in drug delivery, physico-chemical characterisation, analytical chemistry, nanoplatform synthesis and functionalisation, as well as in cellular response evaluation.

After having received a BSc in Physics and Chemistry, followed by a MSc with high honours in Nanotechnologies and Nanobiosciences from the University of Bourgogne in 2010, I received a PhD degree in Physical-Chemistry in 2014. My doctoral thesis included studies on the synthesis of organic/inorganic nanostructures to control the loading and release kinetics of cisplatin and evaluation of cellular uptake and cytotoxic effects of these nanotherapeutics. Complete physico-chemical characterisation of the nanoparticles was achieved (TEM, nitrogen sorption, DLS, zeta potential, ICP-AES, FTIR, UV-Vis, Raman, and NMR spectroscopy).

At the end of my PhD, I joined the MicroTechnologies for Healthcare and Biology Division at CEA-Leti as postdoctoral fellow. I worked on the development and validation of characterization methods for Lipidots®, a solid lipid nanoparticle platform primarily dedicated to in vivo fluorescence imaging and diagnosis of solid tumors as well as drug delivery for both traditional chemotherapeutic and photodynamic agents for cancer therapy. I investigated the drug localization, the lipid quantification, drug/dye encapsulation, release kinetics and leakage profiles to anticipate the nanocarrier behavior in biological and storage media respectively. My work enabled the set-up of an analytical platform suitable for the analysis of nanotherapeutics. This work was performed in a cross-disciplinary environment at the chemistry/biology interface in relation with different European and industrial projects.

At the Advanced Therapies Group, within the School of Dentistry, I am actively working on testing a novel bio-triggered antibiotic delivery system, based on polymer therapeutics, to provide crucial understanding of the effect of polymer conjugation on colistin's nephrotoxicity, and to determine the clinical potential of "nanoantibiotics" for improved antibiotic therapy.

Education and qualifications

  • 2010-2014: PhD in Physical Chemistry University of Bourgogne, Dijon, France
  • 2009-2010: MSc in Nanotechnologies and Nanobiosciences University of Bourgogne, Dijon, France
  • 2008-2009: MSc in Physics and Chemistry University of Bourgogne, Dijon, France
  • 2005-2008: BSc in Physics and Chemistry University of Bourgogne, Dijon, France

Career overview

  • 2017-present: Postdoctoral Research Associate, Cardiff University, School of Dentistry, Cardiff, United Kingdom
    Topic: “Accumulatiom and nephrotoxicity of dextrin-colistin conjugates ”
  • 2014-2016: Postdoctoral Researcher, CEA-Leti, Microtechnologies for Biology and Healthcare Division, Grenoble, France
    Topic: “Development and validation of characterization methods for lipid nanoparticle-based
    delivery systems”
  • 2010-2014: PhD Researcher, University of Bourgogne, Laboratoire Interdisciplinaire Carnot de Bourgogne, Dijon, France
    Topic: “Synthesis of mesoporous silica nanoparticles and encapsulation of cisplatin for targeted
    cancer therapies”
  • 2010: MSc Internship, University of Bristol, HH Wills Physics Laboratory, Bristol, United Kingdom
    Topic: “Inversion and characterization of photonic band gap crystal templates”
  • 2009: MSc Internship, University of Bourgogne, Laboratoire Interdisciplinaire Carnot de Bourgogne, Dijon, France
    Topic: “Synthesis of silica nanoparticles via reverse microemulsion and sol-gel methods for
    pharmaceutical applications”

Professional memberships

  • French Society for Nanomedicine (SFNano)
  • British Society for Nanomedicine (BSNM)

Committees and reviewing

  • 2012-2014: Member of the Laboratoire Interdisciplinaire Carnot de Bourgogne Committee, Dijon, France
  • 2011-2013: PhD student representative at the Department of Chemistry, Dijon, France

External committees

  • 2015: Member of organization committee: European Nanomedicine Meeting 2015, Grenoble, France
  • 2010-2013: Communication Manager at the French Chemical Society, Dijon, France

Teaching

  • 2010-2013: Supervision of laboratory practical work at undergraduate level
    Faculty of Science and Technology, University of Bourgogne
    Faculty of Pharmaceutical Sciences, University of Bourgogne
  • 2007-2009: Physics and Chemistry tutoring to undergraduate students
    Faculty of Science and Technology, University of Bourgogne

Graduate students supervised

  • Sept 2015-Jun 2016: Mathieu Ciancone (MSc Thesis) University Claude Bernard, Lyon, France
  • Feb 2013-Jun 2013: Florent Crevat (MSc Thesis) University of Bourgogne, Dijon, France
  • Oct 2012-Jan 2013: Etienne Cierniak (Engineer Thesis) ESIREM, Dijon, France
  • Feb 2012-Sept 2012: Alexandre Royer (Engineer Thesis) ESIREM, Dijon, France
  • Apr 2012-Jun 2012: Ulrich Legrand (MSc Thesis) University of Bourgogne, Dijon, France
  • Feb 2011-Jun 2011: Amélie Cot (MSc Thesis) University of Bourgogne, Dijon, France
  • Oct 2010-Jan 2011: Manuela Aoukar (Engineer Thesis) ESIREM, Dijon, France
  • Oct 2010-Jan 2011: Romane Sarrazin (Engineer Thesis) ESIREM, Dijon, France

Current project

Accumulation and nephrotoxicity of dextrin-colistin conjugates

Specific aims of this research project are to:

  • assess in vitro cytotoxicity of dextrin-colistin conjugates compared to colistin and CMS
  • determine in vitro cellular uptake, accumulation and localisation of dextrin-conjugates compared to colistin and CMS
  • characterise in vivo distribution and toxicity of dextrin-colistin conjugates compared to colistin and CMS
  • identify lead candidate(s) for further in vivo testing of biodistribution and antimicrobial activity
  • test the efficacy and distribution of dextrin-colistin conjugates in an in vivo model of infection