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Proteomics Facility

Use:
The Proteomics facility has recently benefitted from generous support through SRIF3. We offer state of the art facilities for the protein and peptide seperation, the identification of proteins on a global sacle, the analysis of individual proteins and the characterisation of protein-protein interactions.

The facility presently offers the following resources for:

  • 1D/2D electrophoresis (GE Healthcare Ettan IPGPhor, DALT VI and multiphor systems, Invitrogen mini-gel system)

  • Gel scanning and analysis (GE ImageScanner and Nonliner Dynamics Phoretix 2D Expression Software)

  • 2D fluorescence difference gel electrophoresis (DIGE; GE Typhoon Trio varible mode imager and DeCyder 2D Version 6.5 software)

  • Free flow electrophoresis (BD diagnostics)

  • Lab-on-a-chip microfluidics-based protein electrophoresis and quantification (Agilent 2100 Bioanalyser)

  • Automated spot picking (Ettan Spot Picker) and in-gel trypsin digestion (Ettan digester)

  • State-of-the-art tandem mass spectrometry (MS; Applied Viosystems 4800 MALDI TOF/TOF Analyser, Global Proteom Explorer and Protein Pilot workstation, MarkerView software and MASCOT ite licence).

  • Nano-liquid chromatography (nanoLC; Dionex Ultimate 3000, Probot Spotter and Chromeleon software) for advanced proteomics workflows (geLC-MS, MDLC-MS and quantative proteomics using reagents such as iTRAQ, ICAT and SILAC)

  • Two solutions for surface plasmon resonance (SPR; Biacore T100 and 3000 work station )to study protein-protein (or other macromolecular) interactions (with MALDI target plate spotting facility).

  • Protein purification and production systems (Acta FPLC, Beckman HPLC and small scale fermentor

Location:
Room GF12, Henry Wellcome Building, School of Medicine, Heath Park, Cardiff CF14 4XN

Contact:
Dr Ian Brewis
Email: CBSProteomics@cf.ac.uk
Telephone: +44 (0)29 2074 4006

www.cardiff.ac.uk/cbs/proteomics