The Proteomics facility has recently benefitted from generous support through SRIF3. We offer state of the art facilities for the protein and peptide seperation, the identification of proteins on a global sacle, the analysis of individual proteins and the characterisation of protein-protein interactions.
The facility presently offers the following resources for:
1D/2D electrophoresis (GE Healthcare Ettan IPGPhor, DALT VI and multiphor systems, Invitrogen mini-gel system)
Gel scanning and analysis (GE ImageScanner and Nonliner Dynamics Phoretix 2D Expression Software)
2D fluorescence difference gel electrophoresis (DIGE; GE Typhoon Trio varible mode imager and DeCyder 2D Version 6.5 software)
Free flow electrophoresis (BD diagnostics)
Lab-on-a-chip microfluidics-based protein electrophoresis and quantification (Agilent 2100 Bioanalyser)
Automated spot picking (Ettan Spot Picker) and in-gel trypsin digestion (Ettan digester)
State-of-the-art tandem mass spectrometry (MS; Applied Viosystems 4800 MALDI TOF/TOF Analyser, Global Proteom Explorer and Protein Pilot workstation, MarkerView software and MASCOT ite licence).
Nano-liquid chromatography (nanoLC; Dionex Ultimate 3000, Probot Spotter and Chromeleon software) for advanced proteomics workflows (geLC-MS, MDLC-MS and quantative proteomics using reagents such as iTRAQ, ICAT and SILAC)
Two solutions for surface plasmon resonance (SPR; Biacore T100 and 3000 work station )to study protein-protein (or other macromolecular) interactions (with MALDI target plate spotting facility).
Protein purification and production systems (Acta FPLC, Beckman HPLC and small scale fermentor
Room GF12, Henry Wellcome Building, School of Medicine, Heath Park, Cardiff CF14 4XN
Dr Ian Brewis
Telephone: +44 (0)29 2074 4006