Skip to content
Skip to navigation menu

 

Flow Cytometry Facility

Use:
The aim of the Flow Facility is to provide affordable access to flow cytometric analysis and cell sorting to resident scientists at Cardiff University. To achieve this, we have 4 benchtop flow cytometers (3 FACSCaliburs, 1 FACSCan) and a high speed MoFlo cell sorter, situated in 3 buildings across the Heath Park site. The benchtop flow cytometers are run by Dr. Eddie Wang, while the cell sorting facility is provided by Dr. Chris Pepper. There are dedicated staff to carry out cell sorting, but benchtop flow cytometry involves informal training from experienced users. Charges are applied on a no profit basis and are designed to recover costs for servicing and consumables.

1x FACSCalibur and 1x FACScan

Location:
Flow Cytometry Facility, Henry Wellcome Building, School of Medicine, Heath Park, Cardiff CF14 4XN

1x FACSCalibur

Location:
Dept. of Medical Biochemistry and Immunology, 2nd Floor, Tenovus Building, School of Medicine, Heath Park, Cardiff CF14 4XN

Contact:


1x FACSCalibur

Location:
Nephrology Department, School of Medicine, Heath Park Cardiff CF14 4XN

Contact:


Cell Sorting

The MoFlo is currently available to measure up to 9 parameters simultaneously using 6 colours, forward scatter, side scatter and time. Flow sorting has numerous applications including the following:

  • Identification and isolation (purification) of transfected cells (GFP/TFP/BFP)
  • Isolation of sub populations of cells with different phenotypes for downstream analysis (e.g. DNA, RNA, protein assays and cell culture)
  • Cloning (single cell per well)

For use on projects including:

  • Chromosome sorting
  • Sub-clone sorting
  • B and T cell subset sorting
  • GFP/YFP/BFP transfected cell sorting
  • Cell cycle fraction sorting (i.e. G0/G1, S, G2/M)

Location:
Henry Wellcome Building, School of medicine, Heath Park, Cardiff CF14 4XN

Contact:
Dr Chris Pepper
Email:
CBSFlow@cf.ac.uk

Telephone: +44 (0)29 2074 3482
www.medicine.cf.ac.uk/cbs/