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CITER Cryo-Ultramicrotome


The Leica EM UC6/FC6 allows semi and ultrathin slices or sections to be prepared from biological tissues, polymers, membranes, etc at ambient or low temperature (down to -185°C), for ultrastructural analysis by high resolution transmission electron microscopy. The low temperature sectioning facility (FC6) is invaluable to researchers wishing to carry out immuno-detection of specific components in tissue sections using antibody labelling, particularly where high sensitivity is required. In addition, where target antigens are intolerant to conventional specimen preparation procedures employing resin embedding, the low temperature approach offers major advantages. Cryo-planing of frozen samples is also possible for studies using conventional or cryo-scanning electron microscopy. Ancillary equipment also acquired to support the operation of the EM UC6/FC6 includes a Leica EM KMR2 (balanced break) Glass Knifemaker, Cryotrimming diamond blade and Cryo diamond knife.

Prospective users should already be experienced in ultramicrotomy and will be expected to undertake a brief training session in the use of the equipment in low temperature mode.

Location: School of Optometry & Vision Sciences, College of Biomedical and Life Sciences, Maindy Road, Cardiff. CF24 4HQ

Contact: Prof Andrew J. Quantock