Dr Joachim Bugert
Department of Medical Microbiology & Infectious Diseases,
Cardiff Institute of Infection and Immunity, School of Medicine
029 2074 3583
029 2074 2161
Relevant Key Words:
Antivirals, virus cell biology, influenzavirus, measlesvirus, poxviruses, hepatitis c virus, Organotypic cell
Research Expertise relevant to tissue engineering & repair:
Virus infections of conventional and dynamic cell culture systems. Organotypic cell cultures: skin, lung
Antivirals: We test and develop novel antivirals in collaboration with Prof Chris McGuigan, School of
Pharmacy and Pharmaceutical Sciences. We have patented ddBCNAs with activity versus HCMV,
poxviruses, and measlesvirus via a cellular target (s). We are currently investigating the MoA. We
have established a CL3 cellculture system for replicating hepatitis c virus (pJFH-1) to test candidate
antivirals active against this virus and are interested in comparing innate immune responses directed
against the virus in culture with those induced by HCV vaccine candidates. We are in the process of
establishing a humanized mouse model for HCV in Cardiff and are looking to acquire/develop a liver
Skin focus: My group investigates the properties of Molluscum Contagiosum Virus (MCV), a poxvirus
dedicated to the human skin. MCV causes benign tumors of hyperproliferating keratinocytes only in
the epidermal layer of human skin. The tumors are classified as acanthomas. MCV only replicates in
human epidermal keratinocytes in vivo and for this reason there is no in vitro replication system for
this virus. To find out what makes the virus replicate in human skin and not in conventional epithelial
cell lines, we are studying virus infection of human keratinocytes and in vitro skin equivalents. We
have an ongoing collaboration on MCV skin immunology with Prof Bernhard Moser in the School
of Medicine, and a collaboration with Dr Amanda Tonks on aspects of MCV innate immunity.
Bacteriophages: we have an interest in bacteriophages targetting MDR gram negative
microorganisms and MOTT through their genetic transfer systems. This project is in collaboration
with Dr Mark Toleman in the School of Medicine, and will be tested in a lung tissue model.
Methodology: organotypic cell culture models, virus morphology, entry, virus uncoating,
transcription, genome replication, egress, and cytokine induction via TLR , PKR and RIG
using EM, immunefluorescent / confocal microscopy, PCR, RT-PCR, nucleic acid and protein
blotting techniques, lymphocyte diapedesis, cytokine ELISAs and cytokine bioassays.
We would like to collaborate with groups interested in skin infection, hepatitis C, respiratory
infections, incl MOTT, and bacteriophages in Cardiff and elsewhere.
If you have reagents that may be of interest to the CITER Membership, e.g. cell lines,
microbiological cultures. Please give a representative list below:
Please contact me for cell culture reagents/ cell lines related to skin, liver, lung research.