Dr Deborah J Mason
School of Biosciences, Museum Avenue, Cardiff University
029 2087 4561
Relevant Key Words:
Bone cartilage, Mechanotransduction, Glutamate, Osteoporosis osteoarthritis, Rheumatoid arthritis, Ceramide PKR
Research Expertise relevant to tissue engineering & repair:
My research elucidates new signalling mechanisms that regulate bone and cartilage turnover, to provide therapeutic and diagnostic targets for osteoporosis, rheumatoid arthritis and osteoarthritis. This has led to the discovery of functional glutamatergic signalling in bone and synovium, and revealed new pathways that mediate cytokine- and mechanically- induced cartilage degradation. Modulation of glutamatergic signalling to enhance bone formation and inhibit pathological changes in arthritis, and delineating the role of PKR in arthritis, are current research priorities.
Glutamate Signalling: My work implicating the glutamate transporter GLAST-1 in mechanotransduction, and identifying glutamate as a potential anabolic target in bone is leading to new strategies for increasing bone formation. Our discovery that glutamate signalling mediates pathological responses in rheumatoid arthritis reveals a therapeutic opportunity whereby joint destruction could be prevented using already licensed glutamate receptor antagonists. Cloning and characterisation of the GLAST-1a splice variant is revealing new mechanisms of neurotoxicity.
PKR and ceramide in arthritis: Protein Kinase R(PKR) mediates stress and viral responses. We reported that PKR is activated in osteoarthritis and demonstrated that PKR mediates TNF a -induced degradation of cartilage. We have also shown that ceramide, a cytokine induced second messenger, influences cartilage matrix homeostasis via ERK signalling. These data reveal new mechanisms of cytokine mediated cartilage degradation that may be targeted in treatment or diagnosis of arthritis.
Biomechanics: Mechanical loading is a potent stimulus regulating both formation and degradation of bone and cartilage. Our studies on the effects of mechanical loading in bone and cartilage have implicated glutamatergic signalling, degradative enzymes, and the cytoskeleton in mechanotransduction.
Funded by BBSRC, ARC, Smith and Nephew, PJ Waters Bequest Fund, Welsh Office of Research & Development, The Wishbone Trust, Henry Smiths Charitable Trust
If you have reagents that may be of interest to the CITER Membership, e.g. cell lines, microbiological cultures. Please give a representative list below:
Bone and cartilage cell lines and assays. See publications for details