2014

The Structural Basis of Differential Inhibition of Human Calpain by Indole and Phenyl Based α-Mercaptoacrylic Acids

Sarah E. Adams, Pierre J. Rizkallah, David J. Miller, Emma Robinson, Maurice B. Hallett, Rudolf K. Allemann

J. Struct. Biol., in press (2014). DOI:10.1016/j.jsb.2014.07.004

Role of the Occluded Conformation in Bacterial Dihydrofolate Reductases

Enas M. Behiry, Louis Y. P. Luk, Stella M. Matthews, E. Joel Loveridge and Rudolf K. Allemann

Biochemistry, 53, 4761-4768 (2014). DOI:10.1021/bi500507v

Loop Interactions During Catalysis by Dihydrofolate Reductase from M. profunda

Enas M. Behiry, Rhiannon M. Evans, Jiannan Guo, E. Joel Loveridge and Rudolf K. Allemann

Biochemistry, 53, 4769-4774 (2014). DOI:10.1021/bi500508z

Thermal Adaptation of Dihydrofolate Reductase from the Moderate Thermophile Geobacillus stearothermophilus

Jiannan Guo, Louis Y. P. Luk, E. Joel Loveridge, and Rudolf K. Allemann

Biochemistry, 53, 2855-2863 (2014). DOI:10.1021/bi500238q

Different Dynamical Effects in Mesophilic and Hyperthermophilic Dihydrofolate Reductases

Louis Y. P. Luk, E. Joel Loveridge, and Rudolf K. Allemann

J. Am. Chem. Soc., 136, 6862-6865 (2014). DOI:10.1021/ja502673h

2013

Increased Dynamic Effects in a Catalytically Compromised Variant of Escherichia coli Dihydrofolate Reductase

J. Javier Ruiz-Pernia, Louis Y. P. Luk, Rafael García-Meseguer, Sergio Martí, E. Joel Loveridge, Iñaki Tuñón, Vincent Moliner and Rudolf K. Allemann

J. Am. Chem. Soc., 135, 18689-18696 (2013). DOI:10.1021/ja410519h

Conformational Change and Ligand Binding in the Aristolochene Synthase Catalytic Cycle

Marc W. van der Kamp, Jitnapa Sirirak, Jolanta Żurek, Rudolf K. Allemann and Adrian J. Mulholland

Biochemistry, 52, 8094-8105 (2013). DOI:10.1021/bi400898k

Calpain-1 Inhibitors for Selective Treatment of Rheumatoid Arthritis- What is the Future?

David Miller, Sarah Adams, Maurice B. Hallett, and Rudolf K. Allemann

Future Medicinal Chemistry, 5, 2057-2074 (2013). DOI:10.4155/fmc.13.172

Efficient Terpene Synthase Catalysis by Extraction in Flow,

Oscar Cascón, Gerald Richter, Rudolf K. Allemann and Thomas Wirth

ChemPlusChem, 78, 1334-1337 (2013). DOI:10.1002/cplu.201300303

Unraveling the Role of Protein Dynamics in Dihydrofolate Reductase Catalysis

Louis Y. P. Luk, J. Javier Ruiz-Pernía, William M. Dawson, Maite Roca, E. Joel Loveridge, David R. Glowacki, Jeremy N. Harvey, Adrian J. Mulholland, Iñaki Tuñón, Vincent Moliner and Rudolf K. Allemann

Proc. Natl. Acad. Sci., 110, 16344–16349 (2013). DOI:10.1073/pnas.1312437110

The Semiochemistry of Aphids

John A. Pickett, Rudolf K. Allemann and Michael A. Birkett

Natural Product Reports, 30, 1277-1283 (2013). DOI:10.1039/C3NP70036D

BH3 Helix-Derived Biophotonic Nanoswitches Regulate Cytochrome c Release in Permeabilised Cells

Robert J. Mart, Rachel J. Errington, Catherine L. Watkins, Sally C. Chappell, Marie Wiltshire, Arwyn T. Jones, Paul J. Smith and Rudolf K. Allemann

Molecular Biosystems, 9, 2597-2603 (2013). DOI:10.1039/C3MB70246D

Mechanistic Insights from the Binding of Substrate and Carbocation Intermediate Analogues to Aristolochene Synthase

Mengbin Chen, Naeemah Al-lami, Marine Janvier, Edward L. D'Antonio, Juan A. Faraldos, David E. Cane, Rudolf K. Allemann and David W. Christianson

Biochemistry, 52, 5441-5453 (2013). DOI:10.1021/BI400691V

Effect of Dimerization on Dihydrofolate Reductase Catalysis,

Jiannan Guo, E. Joel Loveridge, Louis Y.P. Luk and Rudolf K. Allemann

Biochemistry, 52, 3881-3887 (2013). DOI:10.1021/bi4005073

Rational Engineering of Plasticity Residues of Sesquiterpene Synthases from Artemisia annua: Product Specificity and Catalytic Efficiency

Jian-Xu Li, Xin Fang, Qin Zhao, Ju-Xin Ruan, Chang-Qing Yang, Ling-Jian Wang, David J. Miller, Juan A. Faraldos, Rudolf K. Allemann, Xiao-Ya Chen and Peng Zhang

Biochemical J., 451, 417-426 (2013). DOI:10.1042/bj20130041

Strategies for the Manipulation of Carbocations by Aristolochene Synthase

David J. Miller and Rudolf K. Allemann

in Isoprenoid Synthesis in Plants and Microorganisms, New Concepts and Experimental Approaches; pg. 171-183; Bach, Thomas J.; Rohmer, Michel (Eds.), Springer, New York, Heidelberg, Dordrecht, London. ISBN 978-1-4614-4062-8 (2013).

Aliphatic 1H, 13C and 15N Chemical Shift Assignments of Dihydrofolate Reductase from the Psychropiezophile Moritella profunda in Complex with NADP+ and Folate

E. Joel Loveridge, Stella M. Matthews, Christopher Williams, Sara B.-M. Whittaker, Ulrich L. Günther, Rhiannon M. Evans, William M. Dawson, Matthew P. Crump and Rudolf K. Allemann

Biomolecular NMR Assignments, 7, 61-64 (2013). DOI:10.1007/s12104-012-9378-x

2012

Probing the Mechanism of 1,4-Conjugate Elimination Reactions Catalyzed by Terpenoid Synthases

Juan A. Faraldos, Amang Li, Verónica González, Fanglei Yu, Mustafa Köksal, David W. Christianson and Rudolf K. Allemann

J. Am. Chem. Soc., 134, 20844-20848 (2012). DOI:10.1021/ja311022s

1H, 13C and 15N Chemical Shift Assignments of Unliganded Bcl-xL and its Complex With a Photoresponsive Bak-derived Peptide

Piotr Wysoczański, Robert J. Mart, E. Joel Loveridge, Christopher Williams, Sara B.-M. Whittaker, Matthew P. Crump and Rudolf K. Allemann

Biomolecular NMR Assignments. 7, 187-191 (2012). DOI:10.1007/s12104-012-9407-9

Chemoenzymatic Preparation of Germacrene Analogues

Oscar Cascón, Sabrina Touchet, David J. Miller, Verónica González, Juan A. Faraldos and Rudolf K. Allemann,

Chem. Commun., 48, 9702-9704 (2012). DOI:10.1039/C2CC35542F

Chemoenzymatic Synthesis of the Alarm Pheromone (+)-Verbenone from Geranyl Diphosphate

Zulfa Yoosuf-Aly, Juan A. Faraldos, David J. Miller and Rudolf K. Allemann

Chem. Commun., 48, 7040-7042 (2012). DOI:10.1039/c2cc32883f

NMR Solution Structure of a Photo-switchable Apoptosis Activating Bak Peptide Bound to Bcl-xL

Piotr Wysoczański, Robert J. Mart, E. Joel Loveridge, Chris Williams, Sara B.-M. Whittaker, Matthew P. Crump and Rudolf K. Allemann

J. Am. Chem. Soc., 134, 7644-7647 (2012). DOI:10.1021/ja211820p

A 1,6-Ring Closure Mechanism for (+)-δ-Cadinene Synthase?

Juan A. Faraldos, David J. Miller, Verónica González, Zulfa Yoosuf-Aly, Oscar Cascón, Amang Li and Rudolf K. Allemann

J. Am. Chem. Soc., 134, 5900-5908 (2012). DOI:10.1021/ja211820p

Evidence that a 'Dynamic Knockout' in Escherichia coli Dihydrofolate Reductase Does Not Affect the Chemical Step of Catalysis

E. Joel Loveridge, Enas M. Behiry, Jiannan Guo and Rudolf K. Allemann

Nature Chemistry., 4, 292-297 (2012). DOI:10.1038/NCHEM.1296

The Role of Aristolchene Synthase in Diphosphate Activation,

Juan A. Faraldos, Verónica González, Rudolf K. Allemann

Chem. Commun., 48, 3230-3232 (2012). DOI:10.1039/C2CC17588F

Design of Photocontrolled RNA-Binding Peptidomimetics

Robert J. Mart, Piotr Wysoczański, Sabine Kneissl, Antonio Ricci, Andrea Brancale, Rudolf K. Allemann

ChemBioChem., 13, 515-519 (2012). DOI:10.1002/cbic.201100800

Volatile Emissions of Scented Alstroemeria Genotypes are Dominated by Terpenes, and a Myrcene Synthase Gene is Highly Expressed in Scented Alstroemeria Flowers

Danilo Aros, Verónica González, Rudolf Allemann, Carsten Müller, Carlo Rosat and Hilary Rogers

J. Ex. Bot., 63, 2739-2752. DOI:10.1093/jxb/err456

Potent Inhibition of Ca2+-dependent Activation of Calpain-1 by Novel Mercaptoacrylates

Sarah E. Adams, Christian Parr, David J. Miller, Rudolf K. Allemann and Maurice B. Hallett

MedChemComm., 3, 566-570. DOI:10.1039/C2MD00280A

Sesquiterpene Synthases: Passive Catalysts or Active Players?

David J. Miller and Rudolf K. Allemann

Natural Product Reports, 29, 60-71 (2012). DOI:10.1039/C1NP00060H

2011

The Role of Large-scale Motions in Catalysis by Dihydrofolate Reductase

E. Joel Loveridge, Lai-Hock Tey, Enas M. Behiry, William M. Dawson, Rhiannon M. Evans, Sara B.-M. Whittaker, Ulrich L. Günther, Christopher Williams, Matthew P. Crump and Rudolf K. Allemann

J. Am. Chem. Soc., 113, 20561-20570 (2011). DOI:10.1021/ja208844j

Reduced Susceptibility of Moritella profunda Dihydrofolate Reductase to Trimethoprim is Not Due to Glutamate 28

E. Joel Loveridge, William M. Dawson, Rhiannon M. Evans, Anna Sobolewska and Rudolf K. Allemann

The Protein Journal, 30, 546-548 (2011). DOI:10.1007/s10930-011-9361-x

Chemical Synthesis of Cell-Permeable Apoptotic Peptides from in vivo Produced Proteins

Thomas Fricke, Robert J. Mart, Catherine L. Watkins, Marie Wiltshire, Rachel J. Errington, Paul J. Smith, Arwyn T. Jones and Rudolf K. Allemann

Bioconj. Chem., 22, 1763-1767 (2011). DOI:10.1021/bc200338u

Templating Effects in Aristolochene Synthase Catalysis: Elimination versus Cyclisation

Juan A. Faraldos, Verónica González, Michael Senske and Rudolf K. Allemann

Org. Biomol. Chem., 9, 6920-6923 (2011). DOI:10.1039/c1ob06184d

Probing Eudesmane Cation-π Interactions in Catalysis by Aristolochene Synthase with Non-canonical Amino Acids

Juan A. Faraldos, Alicja K, Antonczak, Verónica González, Rebecca Fullerton, Eric M. Tippmann and Rudolf K. Allemann

J. Am. Chem. Soc., 133 (35), 13906-13609 (2011). DOI:10.1021/ja205927u

Inhibition of (+)-Aristolochene Synthase with Iminium Salts Resembling Eudesmane Cation

Juan A. Faraldos and Rudolf K. Allemann

Org. Lett., 13 (5), 1202-1205 (2011). DOI:10.1021/ol2000843

Effect of pH on Hydride Transfer by Escherichia coli Dihydrofolate Reductase

E. Joel Loveridge and Rudolf K. Allemann

ChemBioChem, 12 (8), 1258-1262 (2011). DOI:10.1002/cbic.201000794

2010

Solvent Effects on Catalysis by Escherichia coli Dihydrofolate Reductase

E. Joel Loveridge, Lai-Hock Tey and Rudolf K. Allemann

J. Am. Chem. Soc., 132 (3), 1137-1143 (2010). DOI: 10.1021/ja909353c

Intermediacy of Eudesmane Cation during Catalysis by Aristolochene Synthase

Juan A. Faraldos, Benson Kariuki and Rudolf K. Allemann

J. Org. Chem., 75 (4), 1119-1125 (2010). DOI: 10.1021/jo902397v

Highly Site-selective Stability Increases by Glycosylation of Dihydrofolate Reductase

Lai-Hock Tey, E. Joel Loveridge, Richard S. Swanwick, Sabine L. Flitsch and Rudolf K. Allemann

FEBS J., 277 (9), 2171-2179 (2010). DOI: 10.1111/j.1742-4658.2010.07634.x

The Temperature Dependence of the Kinetic Isotope Effects of Dihydrofolate Reductase from Thermotoga maritima Is Influenced by Intersubunit Interactions

E. Joel Loveridge and Rudolf K. Allemann

Biochemistry, 49 (25), 5390-5396 (2010). DOI: 10.1021/bi100761x

Regulation of β-Lactamase Activity by Remote Binding of Heme: Functional Coupling of Unrelated Proteins through Domain Insertion

Wayne R. Edwards, Abigail J. Williams, Josephine L. Morris, Amy J. Baldwin, Rudolf K. Allemann and D. Dafydd Jones

Biochemistry, 49 (31), 6541-6549 (2010). DOI: 10.1021/bi100793y

Catalysis by dihydrofolate reductase from the psychropiezophile Moritella profunda

Rhiannon M. Evans, Enas M. Behiry, Lai-Hock Tey, Jiannan Guo, E. Joel Loveridge and Rudolf K. Allemann

ChemBioChem, 11 (14), 2010-2017 (2010). DOI: 10.1002/cbic.201000341

Click Chemistry for the Generation of Cell Permeable Apoptotic Peptides

Thomas Fricke, Robert J. Mart, Catherine L. Watkins, Arwyn T. Jones, Rudolf K. Allemann

Drug Discovery Today, 15 (23-24), 1088 (2010). DOI: 10.1016/j.drudis.2010.09.375

2009

Cystine-based Scaffolds for Functional Miniature Proteins

Rudolf K. Allemann

In RSC Biomolecular Sciences, Oxidative Folding of Proteins and Peptides, (Johannes Buchner and Luis Moroder, Eds.) Royal Society of Chemistry, pp. 381-395 (2009). ISBN: 978-0-85404-148-0.

6- and 14-fluoro farnesyl diphosphate: mechanistic probes for the reaction catalysed by aristolochene synthase

David J. Miller, Fanglei Yu, David W. Knight and Rudolf K. Allemann

Org. Biomol. Chem., 7 (5) 962-975 (2009). DOI: 10.1039/b817194g

Probing coupled motions in enzymatic hydrogen tunnelling reactions

Rudolf K. Allemann, Rhiannon M. Evans and E. Joel Loveridge

Biochem. Soc. Trans., 37, 349-353 (2009) DOI: 10.1042/bst0370349

Quantum Tunnelling in Enzyme Catalyzed Reactions

Rudolf K. Allemann and Nigel S. Scrutton (Eds.), RSC Biomolecular Sciences

Royal Society of Chemistry (2009), ISBN: 978-0-85404-122-0. Buy the book!

Direct methods for the analysis of quantum-mechanical tunnelling: dihydrofolate reductase

E. Joel Loveridge and Rudolf K. Allemann

in RSC Biomolecular Sciences, Quantum Tunnelling in Enzyme Catalyzed Reactions (Rudolf K. Allemann and Nigel S. Scrutton, Eds.), Royal Society of Chemistry, pp. 179-198 (2009).

Different Reaction Mechanisms for Mesophilic and Thermophilic Dihydrofolate Reductases

E. Joel Loveridge, Enas M. Behiry, Richard S. Swanwick and Rudolf K. Allemann

J. Am. Chem. Soc., 131 (20), 6926-6927 (2009). DOI: 10.1021/ja901441k

Effect of Dimerisation on the Stability and Catalytic Activity of Dihydrofolate Reductase from the Hyperthermophile Thermotoga maritima

E. Joel Loveridge, Robert J. Rodriguez, Richard S. Swanwick and Rudolf K. Allemann

Biochemistry, 48 (25), 5922-5933 (2009). DOI: 10.1021/bi900411a

Crystal Structure of (+)-δ-Cadinene Synthase from Gossypium arboreum and Evolutionary Divergence of Metal Binding Motifs for Catalysis

Heather A. Gennadios, Veronica Gonzalez, Luigi Di Costanzo, Amang Li, Fanglei Yu, David J. Miller, Rudolf K. Allemann and David W. Christianson

Biochemistry, 48 (26), 6175-6183 (2009). DOI: 10.1021/bi900483b

Are the catalytic properties of enzymes from piezophilic organisms pressure adapted?

Sam Hay, Rhiannon M. Evans, Colin Levy, E. Joel Loveridge, Xi Wang, David Leys, Rudolf K. Allemann and Nigel S. Scrutton

ChemBioChem, 10 (14) 2348-2353 (2009). DOI: 10.1002/cbic.200900367

The Role of Arginine 28 in Catalysis by Dihydrofolate Reductase from the Hyperthermophile Thermotoga maritima

E. Joel Loveridge, Giovanni Maglia and Rudolf K. Allemann

ChemBioChem, 10 (16) 2624-2627 (2009). DOI: 10.1002/cbic.200900465

2008

Secondary Kinetic Isotope Effects as Probes of Environmentally-Coupled Enzymatic Hydrogen Tunneling Reactions

Sam Hay, Jiayun Pang, Phillip J. Monaghan, Xi Wang, Rhiannon M. Evans, Michael J. Sutcliffe, Rudolf K. Allemann and Nigel S. Scrutton

ChemPhysChem, 9, 1536-1539 (2008). DOI: 10.1002/cphc.200800291

Stereochemistry of Eudesmane Cation Formation During Catalysis by Aristolochene Synthase from Penicillium roqueforti

David J. Miller, Jiali Gao, Donald G. Truhlar, Neil J. Young, Veronica Gonzalez and Rudolf K. Allemann

Org. Biomol. Chem., 6, 2346-2354 (2008). DOI: 10.1039/b804198a

X-ray crystallographic studies of substrate binding to aristolochene synthase suggest a metal binding sequence for catalysis

Katerina Y. Shishova, Fanglei Yu, David J. Miller, Juan A. Faraldos, Yuxin Zhao, Robert M. Coates, Rudolf K. Allemann, David E. Cane, and David W. Christianson

J. Biol. Chem., 283, 15431-15439 (2008). DOI: 10.1074/jbc.M800659200

Differential Specific Radiation Damage in the Cu(II)- and Pd(II)-bound Forms of an α-Helical Foldamer: A Case Study of Crystallographic Phasing by RIP and SAD

Klaus Fütterer, Raimond B. G. Ravelli, Scott A. White, Andrew J. Nicoll, Rudolf K. Allemann

Acta Cryst. D, 64, 264-272 (2008).DOI: 10.1107/S0907444907065948

Linking the Functions of Unrelated Proteins Using a Novel Directed Evolution Domain Insertion Method

Wayne R. Edwards, Kathy Busse, Rudolf K. Allemann and D. Dafydd Jones

Nucl. Acid Res., 36, e78 (2008). DOI: 10.1093/nar/gkn363

Chemical Wizardry? The Generation of Chemical Diversity in Terpenoid Biosynthesis

Rudolf K. Allemann

Pure Appl. Chem., 80,1791-1798 (2008). DOI: 10.1351/pac200880081791

Solvent Effects on Environmentally Coupled Hydrogen Tunnelling During Catalysis by Dihydrofolate Reductase from Thermotoga maritima

E. Joel Loveridge, Rhiannon M. Evans and Rudolf K. Allemann

Chem. Eur. J., 14, 10782-10788 (2008). DOI: 10.1002/chem.200801804

Peptide-Based Biophotonic Nanoswitches Act as Antagonists of Anti-Apoptotic Bcl-xL

Sabine Kneissl, E. Joel Loveridge, Christopher Williams, Matthew P. Crump and Rudolf K Allemann

ChemBioChem, 9, 3046-3055 (2008). DOI: 10.1002/cbic.200800502

2007

Molecular Dynamics Simulations of the Thermal Unfolding of Thermotoga maritima DHFR

Jiayun Pang and Rudolf K. Allemann

Phys. Chem. Chem. Phys., 9, 718 (2007). DOI: 10.1039/B611210B

myo-Inositol Monophosphate: A Challenging Target for Mood Stabilising Drugs

D.J. Miller and Rudolf K. Allemann

Mini-Reviews in Medicinal Chemistry , 7, 107 (2007). DOI: 10.2174/138955707779802624

Aristolochene Synthase-Catalyzed Cyclization of 2-Fluorofarnesyl-Diphosphate to 2-Fluorogermacrene A

David J. Miller, Fanglei Yu and Rudolf K. Allemann

ChemBioChem, 2007, 15(8), 1819-1825. DOI: 10.1002/cbic.200700219

Probing the Reaction Mechanism of Aristolochene Synthase with 12,13-Difluorofarnesyl Diphosphate

Fanglei Yu, David J. Miller and Rudolf K. Allemann

Chem. Commun., 2007, 40, 4155-4157. DOI: 10.1039/b709562g

Synthetic Efficiency in Enzyme Mechanisms Involving Carbocations: Aristolochene Synthase

Rudolf K. Allemann, Neil J. Young, Shuhua Ma, Donald G. Truhlar, Jiali Gao

J. Am. Chem. Soc, 129, 13008-13013 (2007). DOI: 10.1021/ja0722067

Competitive Inhibition of Aristolochene Synthase by Phenyl-Substituted Farnesyl Diphosophates: Evidence of Active Site Plasticity

David J. Miller, Fanglei Yu, Neil J. Young and Rudolf K. Allemann

Org. Biomol. Chem., 2007, 5, 3287-3298 DOI: 10.1039/B713301B

2006

Coupling of Protein Motions and Hydrogen Transfer during Catalysis by Escherichia coli Dihydrofolate Reductase

Richard Swanwick, Giovanni Maglia, Lai-Hock Tey and Rudolf K. Allemann

Biochem. J., 394, 259-265 (2006). DOI:10.1042/BJ20051464

Hydride Transfer Reaction Catalyzed by Hyperthermophilic Dihydrofolate Reductase is Dominated by Quantum Mechanical Tunneling and is Promoted by Both Inter- and Intramonomeric Correlated Motions

Jiayun Pang, Jingzhi Pu, Jiali Gao, Donald G. Truhlar, and Rudolf K. Allemann

J. Am. Chem. Soc,128 (24), 8015-8023 (2006). DOI:10.1021/ja061585l

Stabilisation of Transition States Prior to and Following Eudesmane Cation in Aristolochene Synthase

Silvia Forcat and Rudolf K. Allemann

Org. Biomol. Chem., (13), 2563-2567 (2006). DOI:10.1039/b604147g

Designed High Affinity Cu2+-Binding α-Helcal Foldamer

Andrew J. Nicoll, David J. Miller, Klaus Fütterer, Raymond Ravelli and Rudolf K. Allemann

J. Am. Chem. Soc, 128, 9187-9193 (2006). DOI:10.1021/ja061513u

Protein Motions during Catalysis by Dihydrofolate Reductases

Rudolf K. Allemann, Rhiannon M. Evans, Lai-Hock Tey, Giovanni Maglia, Jiayun Pang, Robert J. Rodriguez, Paul J. Shrimpton and Richard S. Swanwick

Phil. Trans. Roy. Soc. B, 361, 1317-1321 (2006). DOI:10.1098/rstb.2006.1865

2005

Photocontrol of Specificity of a Miniature Engrailed Homoeodomain

Lucia Guerrero, Oliver S. Smart, G. Andrew Woolley and Rudolf K. Allemann

J. Am. Chem. Soc., 127, 15624-15629, (2005). DOI:10.1021/ja0550428

De novo Design of a Stable N-terminal Helical Foldamer

Andrew J. Nicoll, Christopher J. Weston, Charlotte H. Cureton, Christian Ludwig, Felican Dancea, Neil Spencer Oliver S. Smart, Ulrich L. Günther and Rudolf K. Allemann

Org. Biomol. Chem., 3, 4310-4315 (2005). DOI:10.1039/b513891d

Photoregulation of DNA Binding Dpecificity by MyoD

Lucia Guerrero, Oliver S. Smart, Christopher J. Weston, Darcy C. Burns, G. Andrew Woolley and Rudolf K. Allemann

Angew. Chem., 44, 7778-7782 (2005). DOI:10.1002/anie.200502666

Increased Thermal Stability of Glycosylated Dihydrofolate Reductase

Richard S. Swanwick, Alison M. Daines, Lai-Hock Tey, Sabine L. Flitsch and Rudolf K. Allemann

ChemBioChem, 8, 1338-1340 (2005). DOI:10.1002/cbic.200500103

Synthesis of Homogeneous Site-Selectively Glycosylated Proteins

Richard S. Swanwick, Alison M. Daines, Sabine L. Flitsch and Rudolf K. Allemann

Org. Biomol. Chem., 3, 572-574 (2005). DOI:10.1039/b417133k

2004

Controlling the DNA Binding Specificity of bHLH Proteins through Intramolecular Interactions

Elizabeth C. Turner, Charlotte H. Cureton, Christopher J. Weston, Oliver S. Smart, and Rudolf K. Allemann

Chem. Biol., 11, 69-77 (2004). DOI:10.1016/j.chembiol.2003.12.015

Bis-Benzyl Protected 6-Amino Cyclitols are Poisonous to Pd/C Catalysed Hydrogenolysis of Benzyl Ethers

M. Bashir-Uddin Surfraz, Mahmoud Akhtar and Rudolf K. Allemann

Tet. Lett., 45, 1223-1226 (2004).DOI:10.1016/j.tetlet.2003.11.130

Removal of the Phosphate Group in Mechanism-based Inhibitors of Inositol Monophosphatase Leads to Unusual Inhibitory Activity

David J. Miller, M. Bashir-Uddin Surfraz, Mahmoud Akhtar, David Gani and Rudolf K. Allemann

Org. Biomol. Chem., 2, 671-688 (2004). DOI:10.1039/b312808c

Pivotal Role of Gly 121 in Dihydrofolate Reductase from E. coli: Altered Structure of a Mutant Enzyme May Form Basis of Diminished Catalytic Performance

Richard S. Swanwick, Paul J. Shrimpton, and Rudolf K. Allemann

Biochemistry, 43, 4119-4127 (2004). DOI:10.1021/bi036164k

A Stable Miniature Protein with Oxaloacetate Decarboxylase Activity

Christopher J. Weston, C. H. Cureton, Melanie J. Calvert, Oliver S. Smart and Rudolf K. Allemann

Chem. Biochem., 5, 1075-1080 (2004). DOI:10.1002/cbic.200300805

Nucleophilic and General Acid Catalysis at Physiological pH by a Designed Miniature Esterase

Andrew J. Nicoll and Rudolf K. Allemann

Org. Biomol. Chem, 2, 2175-2180 (2004). DOI:10.1039/b404730c

Dual Role for Phenylalanine 178 during Catalysis by Aristolochene Synthase

Silvia Forcat and Rudolf K. Allemann

Chem. Comm., 2094-2095 (2004). DOI:10.1039/b407932a

Miniature Proteomics: Designing Functional α-Helical Miniature Proteins

Andrew J. Nicoll and Rudolf K. Allemann

Rec. Res. Dev. Chem., 2, 227-243 (2004).

2003

Functional Role for Tyr 31 in the Catalytic Cycle of Chicken Dihydrofolate Reductase

Paul J. Shrimpton, Alex Mullaney and Rudolf K. Allemann

Prot. Struct. Funct. Genet. 51, 216-223 (2003).DOI:10.1002/prot.10370

Structure-Activity Relationship of Human Serum Paraoxonase PON1

Rakesh S. Bargota, Mahmoud Akhtar, Keith Biggadike, David Gani and Rudolf K. Allemann

Bioorg. Med. Chem. Lett. 13, 1623-1626 (2003). DOI:10.1016/S0960-894X(03)00290-7

Evidence for Differential Folding of Farnesylpyrophosphate in the Active Site of Aristolochene Synthase: Single Point Mutation Converts Aristolochene Synthase into a (E)-Beta-Farnesene Synthase

Athina Deligeorgopoulou and Rudolf K. Allemann

Biochemistry, 42, 7741-7747 (2003). DOI:10.1021/bi034410m

Hydride Transfer during Catalysis by Dihydrofolate Reductase from Thermitoga maritima

Giovanni Maglia, Masood H. Javed and Rudolf K. Allemann

Biochem. J., 374, 529-535 (2003).DOI:10.1042/BJ20030412

Stabilisation of Eudesmane Cation by Tryptophan 334 During Aristolochene Synthase Catalysis

Athina Deligeorgopoulou, Silvia Forcat, Susan E. Taylor and Rudolf K. Allemann

Chem. Comm., 2162-2163 (2003). DOI:10.1039/b306867f

Product like Inhibitors of Inositol Monophosphatase

M. Bashir-Uddin Surfraz, David J. Miller, David Gani and Rudolf K. Allemann

Tet. Lett., 43 (41), 7677-7679 (2003). DOI:10.1016/S0040-4039(03)01878-1

Evidence for Environmentally Coupled Hydrogen Tunnelling During Dihydrofolate Reductase Catalysis

Giovanni Maglia and Rudolf K. Allemann

J. Am. Chem. Soc., 125, 13372-13373 (2003). DOI:10.1021/ja035692g

2002

Design of a Folded, Conformationally Stable Oxaloacetate Decarboxylase

Susan E. Taylor, Trevor J. Rutherford and Rudolf K. Allemann

Perkin Trans. 2, 751-755 (2002). DOI:10.1039/b200057c

Role of Water in the Catalytic Cycle of E. coli Dihydrofolate Reductase

Paul J. Shrimpton and Rudolf K. Allemann

Prot. Sci., 11, 1442-1451 (2002). DOI:10.1110/ps.5060102

Germacrene A is a Product of the Aristolochene Synthase Mediated Conversion of Farnesylpyrophosphate to Aristolochene

Melanie J. Calvert, Peter. R. Ashton and Rudolf K. Allemann

J. Am. Chem. Soc., 124, 11636-11641 (2002). DOI:10.1021/ja020762p

Tyrosine 92 of Aristolochene Synthase Directs Cyclisation of Farnesyl-Pyrophosphate

Melanie J. Calvert, Susan E. Taylor and Rudolf K. Allemann

Chem. Comm., 2384-2385 (2002). DOI:10.1039/b206517g

2001

Synthesis and Characterisation of a Peptide with Oxaloacetate Decarboxylase Activity

Susan E. Taylor, Trevor J. Rutherford and Rudolf K. Allemann, Design

Bioorg. Med. Chem. Lett., 11, 2631-2635 (2001). DOI:10.1016/S0960-894X(01)00519-4

2000

Thermodynamics of DNA Binding Reaction of MM17, a Single Chain Dimer of Transcription Factor MASH-1

Martin Sieber and Rudolf K. Allemann

Nucl. Acids Res., 28, 2122-2127 (2000). DOI:10.1093/nar/28.10.2122

1999

Transcription Factors

Rudolf K. Allemann

Encyclopedia of Molecular Biology, T. E. Creighton (ed.), Wiley, pp. 2586-2593 (1999).

Acquisition of Myogenic Specificity Through Replacement of Two Amino Acid Residues of MASH-1 and Introduction of One Additional alpha-Helical Turn

C. Dezan, Daniel Meierhans, Andreas G. E. Künne and Rudolf K. Allemann

Biol. Chem., 380, 705-710 (1999). DOI:10.1515/BC.1999.088

1998

Single Chain Dimers of MASH-1 Bind DNA with Enhanced Affinity

Martin Sieber and Rudolf K. Allemann

Nucl. Acids Res., 26, 1408-1413 (1998). DOI: 10.1093/nar/26.6.1408

Thermodynamics of the DNA Binding Reaction of Transcription Factor MASH-1

Andreas G. E. Künne, Martin Sieber, Daniel Meierhans and Rudolf K. Allemann

Biochemistry, 37, 4217-4223 (1998). DOI:10.1021/bi9725374

Arginine 348 is a Major Determinant of the DNA Binding Specificity of Transcription Factor E12

Martin Sieber and Rudolf K. Allemann

Biol. Chem., 379, 731-735 (1998). DOI:10.1515/bchm.1998.379.6.721

The N-Terminal Methionine is a Major Determinant of the DNA Binding Specificity of MEF-2C

Daniel Meierhans and Rudolf K. Allemann

J. Biol. Chem., 273, 26052-26060 (1998). DOI:10.1074/jbc.273.40.26052

1997

Covalently Linking MASH-1 BHLH Subunits Enhances Specificity of DNA Binding

Andreas G. E. Künne, and Rudolf K. Allemann

Biochemistry, 36, 1085-1091 (1997). DOI:10.1021/bi962185l

High Level Expression in Soluble Form, One Step Purification, and Characterisation of DNA Binding Domain of MEF-2C

Daniel Meierhans and Rudolf K. Allemann

Prot. Express. Purif., 11, 297-303 (1997). DOI:10.1006/prep.1997.0795

High Affinity Binding of MEF-2C depends on DNA Bending

Daniel Meierhans, Martin Sieber and Rudolf K. Allemann

Nucl. Acids Res., 25, 4537-4544 (1997). DOI:10.1093/nar/25.22.4537

1996

MyoD Displays Modest DNA Binding Specificity

Andreas G. E. Künne, Daniel Meierhans and Rudolf K. Allemann

FEBS Lett., 391, 79-83 (1996). DOI:10.1016/0014-5793(96)00707-7

1995

DNA Binding Specificity of the Basic-Helix-Loop-Helix Protein MASH-1

Daniel Meierhans, Chirine el Ariss, Margrit Neuenschwander, Martin Sieber, Joseph F. Stackhouse and Rudolf K. Allemann

Biochemistry, 34, 11026-11036 (1995). DOI:10.1021/bi00035a008

1993

Design, Synthesis, Structure Analysis, and Catalytic Activity of Oxaldie: An Artificial Enzyme with Nature-like Structure

Kai Johnson, Rudolf K. Allemann, Hans Widmer and Steven A. Benner

Nature, 365, 530-532 (1993). DOI:10.1038/365530a0

1991

A Hybrid of Bovine Pancreatic Ribonuclease and Human Angiogenin: An External Loop as a Module Controlling Substrate Specificity?

Rudolf K. Allemann, Scott R. Presnell and Steven A. Benner

Prot. Eng. 4, 831-835 (1991). DOI:10.1093/protein/4.7.831

Multiple Spatially-Specific Enhancers are Required to Reconstruct the Pattern of Hox-2.6 Gene Expression

Jenny Whiting, Heather Marshall, Martyn Cook, Robb Krumlauf, Peter W. J. Rigby, David Stott, and Rudolf K. Allemann

Genes and Development 5, 2048-2059 (1991). DOI:10.1101/gad.5.11.2048

1989

The Return of Pancreatic Ribonucleases

Steven A. Benner and Rudolf K. Allemann

Trend. Biochem. Sci., 14 (10), 396 (1989). DOI:10.1016/0968-0004(89)90282-X

1988

A Stereochemical Profile of the Dehydrogenases of Drosophila melanogaster

Rudolf K. Allemann, Rebecca Hung and Steven A. Benner

J. Am. Chem. Soc., 110, 5555-5560 (1988). DOI:10.1021/ja00224a046