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Prof Eshwar Mahenthiralingam  -  PhD

The aim of my group’s research is to dissect the natural biology and virulence of opportunistic bacterial pathogens, using molecular and genomic research strategies. In particular, we have focussed on Pseudomonas aeruginosa and Burkholderia cepacia complex bacteria which cause problematic lung infections in people with cystic fibrosis (CF), with notable discoveries from these studies including the following.

Advances in cystic fibrosis microbiology:

  • Adaption of P. aeruginosa to a non-motile phenotype during chronic infection in CF. In 1994, we described that approximately 40% of P. aeruginosa CF isolates loose their ability to express the bacterial flagellum during their adaption to chronic lung infection (see Mahenthiralingam et al. 1994).
  • PCR-fingerprinting methods and the molecular epidemiology of CF pathogens.We were the first to develop Random Amplified Polymorphic DNA (RAPD) typing schemes for P. aeruginosa and B. cepacia complex bacteria (See Mahenthiralingam et al. 1996). These pioneering studies were expanded to several national and international surveys of CF pathogen epidemiology and facilitated the development of robust infection control guidelines.
RAPD transmission


  • A DNA marker for transmissible B. cepacia complex bacteria. From RAPD analysis, we identified a fragment of DNA designated the “B. cepacia Epidemic Strain Marker” (BCESM; see Mahenthiralingam et al. 1997) from which PCR diagnostics were developed and applied by international researchers to track problematic strains and aid CF infection control.
  • Rapid DNA diagnostics for B. cepacia complex species identification. In 1997 “B. cepacia” isolates were shown to belong to multiple, difficult to identify, bacterial species. We developed the first accurate molecular species tests using the recA gene, a method that became one of the global standards for Burkholderia taxonomy (see Mahenthiralingam et al. 2000 and Payne et al. 2005).
recA tree
  • The identification that B. cenocepacia could replace B. multivorans infection in people with CF. Prior to 1995, “B. cepacia” infected CF individuals were often cohorted at treatment clinics. Using both RAPD and recA analysis, we were able to identify strain transmission, super-infection over B. multivorans and poor clinical outcome associated with B. cenocepacia infection (see Mahenthiralingam et al. 2001); again this key understanding helped improved infection control in CF.
  • Identification of the first B. cenocepacia pathogenicity island. After collaborative initiation of the first B. cepacia complex genome sequence in 2000 (published in 2009; see Holden et al. 2009), we used the sequence data to show the “B. cepacia epidemic strain marker” was in fact part of a novel B. cenocepacia pathogenicity island encoding virulence and important quorum sensing regulatory genes (see Baldwin et al. 2004).
cenocepacia island
  • Collaborative development of a multilocus sequence typing (MLST) scheme for the B. cepacia complex. As sargasso an expansion of recA analysis, we played an integral part in the collaborative development of a B. cepacia complex MLST scheme (see Baldwin et al. 2005; see Several ground-breaking findings stemmed from MLST analysis including: (i) the linkage of a microbial metagenome to a cultivable, globally distributed Bcc strain (see Mahenthiralingam et al. 2006); (ii) the unequivocal demonstration of clonality between environmental and clinical Burkholderia strains (see Baldwin et al. 2007); (iii) multiple  detailed studies of B. cepacia population biology; (iv) characterisation of multiple new B. cepacia complex species, including B. lata and B. contaminans (see Vanlaere et al. 2009) and, (v) the expansion of the MLST scheme to include all Burkholderia species (see Spilker et al. 2009).

Advances in Burkholderia genomics:

  • Genomic analysis of Burkholderia bacteria. We have collaboratively initiated and characterised multiple Burkholderia genomes, including assisting in the annotation of putative virulence factors in the B. xenovorans genome (see Chain et al. 2006), the first signature tagged transposon mutagenesis study of B. vietnamiensis in ecological settings (see O’Sullivan et al. 2007) and first publication of a B. cepacia complex genome, that of B. cenocepacia J2315 (see Holden et al. 2009).
  • Global gene expression analysis of Bcc bacteria. We were involved in the collaborative development of a B. cenocepacia J2315 microarray and hence were able for the first time to examine the global gene expression of this pathogen during growth in CF sputum (see Drevinek et al. 2008). Subsequently, my group to ran an international B. cenocepacia microarray program with funding from the US Cystic Fibrosis Foundation Therapeutics that has led to multiple novel insights into the gene expression behind virulence, antibiotic resistance, quorum sensing and biofilm lifestyles of these bacteria.
  • Stable evolution of antibiotic resistance in B. cenocepacia. We recently showed that when the genome reference Stable evolution of antibiotic resistance in B. cenocepacia isolate, B. cenocepacia J2315, is exposed to single antibiotic, stable resistance to that antibiotic and multiple others will occur (Sass et al. 2011). This finding was corroborated by the analysis of clones that had naturally circulated within the CF population and caused an outbreak of infection in 2008. These recent clinical isolates had evolved the same antibiotic resistance profile over the 19 years that had past since the isolate of J2315 in 1989. Microarray analysis demonstrated that the gene expression changes behind the evolved resistance were stable and not lost in the absence of the antibiotic.

Advances in industrial and applied microbiology:

Several projects with industrial partners have been carried out applying our expertise in molecular microbiology to questions in applied microbiology:

  • Bacterial contamination of fuels. As part of an NERC-CASE PhD studentship, Judith White worked with ECHA Microbiology Ltd. (, using DNA sequence-based identification approaches and cultivation-independent molecular methods to systematically survey the diversity of bacterial fuel contaminants (see White et al. 2011). A notable finding from this study was the over-representation of Pseudomonas species bacteria in the cultivation-based analysis which was not evident from the cultivation-independent bacterial diversity analysis.
  • Bacterial contamination in industry. We have also been working with Unilever Research and Development, Port Sunlight, ( who were the CASE partners for a BBSRC studentship that sponsored Laura Rushton (ne Thomas). Laura examined microbial contamination in industrial processes and bacterial preservative resistance and is now continuing this research as a part of Technology Strategy Board sponsored Knowledge Transfer Partnership. Rebecca Weiser has also started a BBSRC PhD studentship that is CASE funded by Unilever Research and Development, to examine Pseudomonas aeruginosa preservative resistance.
RAPD fingerprinting analysis of bacterial probiotics.
  • Genetic fingerprint and tracking of bacterial probiotics. With funding from Cultech Ltd., we employed our knowledge of Random Amplified Polymorphic DNA typing methods to develop a strategy for tracking Lactic Acid Bacteria during the consumption of probiotics (see Mahenthiralingam et al. 2009). Matthew Bull is continuing this work as part of a BBSRC-CASE PhD studentship with Cultech Ltd.
  • Chemistry & Biology
  • Burkholderia: A source for antibiotic discovery. Recently we were able to show that novel polyketide antibiotics are produced by B. cepacia complex bacteria which kill other Burkholderia bacteria and multidrug resistant bacteria. The biosynthetic pathway and chemistry of one of these antibiotics, enacyloxin IIa, was characterised in detail (see Mahenthiralingam et al. 2011) and we hope this exciting discovery lead to further research on Burkholderia as a source of novel antibiotics.



  • International Burkholderia cepacia Working Group (IBCWG; I collaborate widely with many participants in this international working group and have also organized the 1999  (Banff, Canada) and 2003 annual meetings (London, UK)
  • Prof. David Speert and Dr. James Zlosnik, University of British Columbia, Vancouver, Canada
  • Prof. Peter Vandamme and Prof. Tom Coenye, University of Gent, Gent, Belgium
  • Prof. John LiPuma, University of Michigan, Ann Arbor, Michigan USA
  • Prof. Leo Eberl, University of Zurich, Zurich, Switzerland
  • Prof. Miguel Valvano, Centre for Infection and Immunity, Queen’s University of Belfast, Belfast
  • Dr. Pavel Drevinek, 2nd Medical School, University of Prague, Prague, Czech Republic


  • Prof. Andrew Weightman, Prof. Lynne Boddy, Dr.   Colin Berry, Dr. Hilary Rogers and Dr. Julian Marchesi at Cardiff School of Biosciences, Cardiff University
  • Dr. Adam Baldwin and Prof. Chris Dowson, University of Warwick, Coventry
  • Prof Gregory Challis and Dr Lijiang Song, Department of Chemistry, University of Warwick, Coventry
  • Prof Julian Parkhill, The Sanger Institute, Hinxton, Cambridge
  • Dr. Andrew Jones, Dr. William Flight and Dr. Rowland Bright-Thomas, Manchester Adult Cystic Fibrosis Centre, University Hospital of South Manchester NHS Foundation Trust, Manchester
  • Dr. Sue Plummer, Cultech Ltd, Swansea, UK
  • The UK CF Microbiology Consortium (
  • NISCHR Microbiology and Infection Translational Research Group (MITREG) and Cystic Fibrosis Research Group (


  • UK Cystic Fibrosis Trust
  • US Cystic Fibrosis Foundation
  • The Leverhulme Trust
  • Technology Strategy Board & Knowledge Transfer Partnerships
  • Natural Environment Research Council
  • Biolology and Biotechnology Research Council
  • Society for General Microbiology
  • Society for Applied Microbiology
  • Cultech Ltd.
  • Echa Microbiology Ltd.
  • Unilever Research and Development, UK

Current group members:

  • Dr. Laura Rushton (ne Thomas) (
  • Mr. Matthew Bull (
  • Ms. Rebecca Weiser (

Past group members:

  • Dr. Andrea Sass
  • Dr. Othman Boaisha
  • Dr. Judith White
  • Dr. Helen Rose
  • Dr. Pavel Drevinek
  • Dr. Louise O’Sullivan
  • Dr. Adam Baldwin
  • Dr. Deborah Lewis
  • Dr. George Payne
  • Dr. Saber Yezli
  • Ms. Angela Marchbank