Third generation multiphoton microscope

This award will fund a 'third generation' multiphoton microscope at Cardiff University.

This equipment is the third-generation evolution of the Coherent Antistokes Raman Scattering (CARS) and four-wave mixing (FWM) imaging capabilities presently available at Cardiff University. 

Invented and developed within the biophotonics group led by Professor Paola Borri (School of Biosciences) and Professor Wolfgang Langbein (School of Physics and Astronomy) these are unique to the UK.

The equipment will enable rapid label-free chemically-specific CARS microscopy and Stimulated Raman Scattering (SRS) of biomolecules (lipids, proteins, DNA) over a wider vibrational frequency range and with reduced photodamage compared to previous generation systems. 

The proposed multimodal platform will also enable background-free sensitive imaging of single small metallic nanoparticles (NPs) via resonant FWM detection in the same instrument and will include state-of-the art fluorescence modalities, namely:

  • two-photon fluorescence (TPF)
  • lifetime imaging (FLIM)
  • fluorescence correlation spectroscopy (FCS)
  • super-resolution microscopy via Photo-Activated Localisation Microscopy (PALM).

The CARS/FWM imaging capabilities have generated major research impact within Cardiff, the UK and internationally and are run at full capacity through a wide network of collaborations. 

The equipment funded through this award is in response to a high user-demand, and will enable this technology to be expanded into a multi-user facility for multi-disciplinary world-class research in physics, chemistry, material sciences and the life sciences.

People

Funding

The Engineering and Physical Sciences Research Council (EPSRC)